| Identification | More | [Name]
Thiazolyl Blue | [CAS]
298-93-1 | [Synonyms]
3-(4,5-DIMETHYL-2-THIAZOL-2-YL)-2,5-DIPHENYL-2H-TETRAZOLIUM BROMIDE
3-(4,5-DIMETHYL-2-THIAZOLYL)-2,5-DIPHENYL-2H-TETRAZOLIUM BROMIDE
3-(4,5-DIMETHYL-2-THIAZOLYL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE
3-(4,5-DIMETHYLITHIAZOLYL-2)-2,5-DIPHENYL MONOTETRAZOLIUM BROMIDE
3-(4,5-DIMETHYLTHIAZOL-2-YL)-2,5-DIPHENYL-2H-TETRAZOLIUM BROMIDE
3,[4,5-DIMETHYLTHIAZOL-2-YL]-2,5-DIPHENYLTETRAZOLIUM BROMIDE
3-(4,5-DIMETHYLTHIAZOL-2-YL)-2,5-DIPHYL-2 H-TETRAZOLIUM BROMIDE
3-(4,5-DIMETHYLTHIAZOLYL-2)-2,5-DIPHENYL TETRAZOLIUM BROMIDE
METHYLTHIAZOLETETRAZOLIUM
METHYLTHIAZOLYLDIPHENYL-TETRAZOLIUM BROMIDE
MTT
THIAZOLE BLUE
THIAZOLYL BLUE
THIAZOLYL BLUE TETRAZOLIUM BROMIDE
THIAZOYL BLUE TETRAZOLIUM BROMIDE
2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2h-tetrazoliubromide
2H-Tetrazolium,2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-,bromide
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2h-tetrazoliubromide
mmttetrazolium
thiazolylbluemonotetrazolium | [EINECS(EC#)]
206-069-5 | [Molecular Formula]
C18H18BrN5S | [MDL Number]
MFCD00011964 | [Molecular Weight]
416.34 | [MOL File]
298-93-1.mol |
| Chemical Properties | Back Directory | [Appearance]
yellow crystalline powder | [Melting point ]
195 °C (dec.) (lit.) | [density ]
1.4886 (estimate) | [storage temp. ]
2-8°C
| [solubility ]
methanol: 5 mg/mL, clear
| [form ]
powder
| [color ]
Yellow to orange | [Water Solubility ]
Slightly soluble | [Sensitive ]
Light Sensitive | [λmax]
378 nm, 242 nm | [BRN ]
3825277 | [Stability:]
Hygroscopic | [Biological Applications]
Cell viability assay; microbial growth assays; DNA quantification assays; tissue viability assays; detecting enzymes; measuring membrane potential; treating Alzheimer’s disease,asthma,cancer | [InChIKey]
AZKSAVLVSZKNRD-UHFFFAOYSA-M | [CAS DataBase Reference]
298-93-1(CAS DataBase Reference) | [EPA Substance Registry System]
298-93-1(EPA Substance) |
| Safety Data | Back Directory | [Hazard Codes ]
Xi,T,Xn | [Risk Statements ]
46-68-36/37/38 | [Safety Statements ]
S22:Do not breathe dust .
S24/25:Avoid contact with skin and eyes .
S53:Avoid exposure-obtain special instruction before use .
S45:In case of accident or if you feel unwell, seek medical advice immediately (show label where possible) . | [WGK Germany ]
3 | [RTECS ]
XF8060000 | [F ]
3-8-10 | [TSCA ]
Yes | [HS Code ]
29341000 | [Hazardous Substances Data]
298-93-1(Hazardous Substances Data) |
| Hazard Information | Back Directory | [Chemical Properties]
yellow crystalline powder | [Uses]
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems. | [Definition]
ChEBI: Thiazolyl Blue is the bromide salt of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium. It has a role as a dye and a colorimetric reagent. It contains a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium. | [General Description]
Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. It is used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells. As an electron acceptor, it is utilized for studying nicotinamide adenine dinucleotide phosphate (NADP)-dependent dehydrogenases. | [Biochem/physiol Actions]
Cell permeable: yes | [storage]
+4°C | [Purification Methods]
It is recrystallised by dissolving in MeOH containing a few drops of HBr and then adding dry Et2O to complete the crystallisation, wash the needles with Et2O and dry them in a vacuum desiccator over KOH. [Beyer & Pyl Chem Ber 87 1505 1954, Beilstein 27 III/IV 6045.] |
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