| Identification | More | [Name]
2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol | [CAS]
6976-37-0 | [Synonyms]
2,2-BIS(HYDROXYETHYL)-(IMINOTRIS)-(HYDROXYMETHYL)-METHANE
2,2-BIS(HYDROXYMETHYL)-2,2',2''-NITRILOETHANOL
2,2-BIS-(HYDROXYMETHYL)-2,2',2'-NITRILOTRIETHANOL
2,2-BIS(HYDROXYMETHYL)-2,2',2''-NITRILOTRIETHANOL
2-BIS[2-HYDROXYETHYL]AMINO-2-[HYDROXYMETHYL]1,3-PROPANEDIOL
BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE
BIS(2-HYDROXYETHYL)IMINOTRIS(HYDROXYMETHYL)METHANE
BIS(2-HYDROXYETHYL)IMINO-TRIS(HYDROXYMETHYL)PROPANE
BIS(HYDROXYETHYL)IMINO-TRIS-(HYDROXYMETHYL)-METHANE
''BIS-TRIS''
BIS-TRIS
BIS-TRIS BUFFER
2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-3-propanediol
Bis Tris Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane
Bis Tris, Molecular Biology Grade Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane, Molecular Biology Grade
Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane~Bis-TRIS
Bis(2-hydroxyethyl)-imino-tris-(hydroxymethyl)-methane biological buffer
bis-tris cell culture tested
bis-tris free base
bis-tris free base sigmaultra | [EINECS(EC#)]
230-237-7 | [Molecular Formula]
C8H19NO5 | [MDL Number]
MFCD00002853 | [Molecular Weight]
209.24 | [MOL File]
6976-37-0.mol |
| Chemical Properties | Back Directory | [Appearance]
White/clear crystalline powder | [Melting point ]
104 °C(lit.)
| [Boiling point ]
466.2±40.0 °C(Predicted) | [density ]
1.346±0.06 g/cm3(Predicted) | [vapor pressure ]
0Pa at 25℃ | [storage temp. ]
Store at RT. | [solubility ]
H2O: 1 M at 20 °C, clear, colorless
| [form ]
Solution | [color ]
Yellow to orange | [Odor]
Odorless | [PH]
9.0-10.1 (20-25℃, 1M in water) | [PH Range]
5.8 - 7.2 | [pka]
6.5(at 25℃) | [biological source]
synthetic | [Water Solubility ]
soluble | [Sensitive ]
Air Sensitive | [λmax]
λ: 280 nm Amax: 0.15 | [Detection Methods]
MP,NMR | [BRN ]
2205275 | [Major Application]
diagnostic assay manufacturing | [Cosmetics Ingredients Functions]
BUFFERING | [InChI]
1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2 | [InChIKey]
OWMVSZAMULFTJU-UHFFFAOYSA-N | [SMILES]
OCCN(CCO)C(CO)(CO)CO | [LogP]
-2.26 at 20℃ | [CAS DataBase Reference]
6976-37-0(CAS DataBase Reference) | [Storage Precautions]
Air sensitive | [EPA Substance Registry System]
6976-37-0(EPA Substance) | [Absorption]
cut-off at 270nm in H2O at 1M |
| Questions And Answer | Back Directory | [Preparation]
First, 100 ml of water and 120 g of tris(hydroxymethyl)aminomethane were added to a 1000 ml four-necked flask and heated with stirring. Then, when the temperature reached 80°C, 25 g of anion exchange resin was added, and 200 g of molten ethylene carbonate was added dropwise. Specifically, the 200 g of molten ethylene carbonate was added dropwise over three hours. Simultaneously, the reaction was maintained at 80°C for 20 hours. After the reaction was stopped, the reaction solution was filtered to remove the anion exchange resin and washed with 50 ml of pure water. Then, the filtrate was adjusted to pH 9.5 using a 30% NaOH solution, and then dehydrated under reduced pressure (0.08 MPa) at 78°C-100°C to obtain an oily substance. This was then dissolved in 200 ml of ethanol, cooled to precipitate crystals, and filtered to obtain the crude product. Finally, the crude product was recrystallized from ethanol and dried to obtain 88 g of BIS-Tris. The final product was a white crystal with a yield of 42.4%. |
| Safety Data | Back Directory | [Symbol(GHS) ]
GHS05 | [Signal word ]
Danger | [Hazard statements ]
H318 | [Precautionary statements ]
P280-P305+P351+P338+P310 | [Hazard Codes ]
Xi,Xn | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin .
R21/22:Harmful in contact with skin and if swallowed .
R20/21/22:Harmful by inhalation, in contact with skin and if swallowed . | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing .
S36/37/39:Wear suitable protective clothing, gloves and eye/face protection .
S22:Do not breathe dust . | [WGK Germany ]
3
| [F ]
10-23 | [TSCA ]
Yes | [REACH Registrations]
Active | [HS Code ]
29221980 | [Storage Class]
11 - Combustible Solids | [Hazard Classifications]
Eye Irrit. 2 |
| Hazard Information | Back Directory | [Chemical Properties]
White/clear crystalline powder | [Uses]
It is used as a biological buffer, organic synthesize, chemical & pharmaceutical intermediate. It is suitable for fractionation of hemoglobin. | [Definition]
ChEBI: Bis-tris is a buffer substance useful for calibration of glass electrodes and for the preparation of the biochemical and biological buffer solutions; pKa = 6.46 at 25 ℃. It has a role as a buffer. It is functionally related to a member of tris. | [Flammability and Explosibility]
Notclassified | [Purification Methods]
Crystallise BIS-TRIS from hot 1-butanol and dry it in a vacuum at 25o. N,N-Bis-(2-hydroxyethyl)glycine (BICINE) See in “Amino Acids and Peptides”, Chapter 6. |
| Well-known Reagent Company Product Information | Back Directory | [Acros Organics]
2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, for biochemistry, 99+%(6976-37-0) | [Alfa Aesar]
2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, 98+%(6976-37-0) | [Sigma Aldrich]
6976-37-0(sigmaaldrich) | [TCI AMERICA]
Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane [Good's buffer component for biological research],>99.0%(LC)(T)(6976-37-0) |
|
| Company Name: |
J & K SCIENTIFIC LTD.
|
| Telephone: |
18210857532 18210857532 |
| Website: |
https://www.jkchemical.com |
| Company Name: |
Alfa Aesar
|
| Telephone: |
400-6106006 |
| Website: |
http://chemicals.thermofisher.cn |
| Company Name: |
Energy Chemical
|
| Telephone: |
400-0056266 |
| Website: |
www.energy-chemical.com |
|