| Identification | More | [Name]
3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate | [CAS]
82473-24-3 | [Synonyms]
3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-2-HYDROXY-1-PROPANESULFONATE
3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-2-HYDROXY-1-PROPANE SULPHONATE
CHAPSO
3-((3-cholamidopropyl)dimethylammonio)-2-hydroxy-
CHAPSO 3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate
CHAPSO SIGMAULTRA
CHAPSO ELECTROPHORESIS REAGENT
1-Propanaminium, 2-hydroxy-N,N-dimethyl-3-sulfo-N-3-(3.alpha.,5.beta.,7.alpha.,12.alpha.)-3,7,12-trihydroxy-24-oxocholan-24-ylaminopropyl-, inner salt
CHAPSO,ELECTROPHORESISGRADE
3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-2-HYDROXY-1-PROPANESULPHONATE (ULTRAPURE)
3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulphonate (Ultrapure) >99.0%
CHAPSO extrapure | [EINECS(EC#)]
617-338-5 | [Molecular Formula]
C32H58N2O5S | [MDL Number]
MFCD00081079 | [Molecular Weight]
582.88 | [MOL File]
82473-24-3.mol |
| Chemical Properties | Back Directory | [Appearance]
White crystalline powder | [Melting point ]
184-186 °C(lit.)
| [density ]
1.0453 (rough estimate) | [refractive index ]
1.6900 (estimate) | [storage temp. ]
2-8°C
| [solubility ]
H2O: 10 mg/mL at 20 °C, clear, colorless
| [form ]
White solid | [color ]
White crystalline | [Odor]
Odorless | [Water Solubility ]
10 g/l at 20 °C | [Merck ]
13,2052 | [BRN ]
5842642 | [CAS DataBase Reference]
82473-24-3(CAS DataBase Reference) |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing . | [WGK Germany ]
3
| [F ]
3-10 |
| Hazard Information | Back Directory | [Chemical Properties]
White crystalline powder | [Application]
3-(cholamidopropyl)(dimethylammonio)-2-hydroxy-1-propanesulfonate (CHAPSO) may be used in bicelle preparation for β2-adrenergic receptor reconstitution.
CHAPSO has been used:
in a study to assess the processes of liposome solubilization and reconstitution of two transport proteins
in a study to investigate a novel bicelle system for orienting macromolecules over a wide temperature range
as a mixture with dilauroyl phosphatidylcholine (DLPC) in solid-state nuclear magnetic resonance (NMR) technique to study membrane-associated proteins
A nondenaturing zwitterionic detergent with characteristics similar to CHAPS, although it is more soluble due to a more polar head group. Useful for the solubilization of integral membrane proteins. | [Uses]
3-(cholamidopropyl)(dimethylammonio)-2-hydroxy-1-propanesulfonate (CHAPSO) may be used in bicelle preparation for β2-adrenergic receptor reconstitution. | [Uses]
Nondenaturing biological detergent. | [General Description]
Unique detergent with high CMC that solubilizes membrane-bound proteins in their native state. Capable of solubilizing opiate receptor to a state exhibiting reversible binding of opiates. Has higher solubility than CHAPS because of its more polar head group. Micellar average MW: 7000 Aggregation number: 11 | [Purification Methods]
Dissolve this zwitterionic detergent (~ 60g) in 40% aqueous MeOH and stir it with the mixed-bed ion-exchange resin RG-501 x 8 (100g, Bio-Rad) until the pH of the supernatant is ~ 7. Filter off the resin and evaporate the filtrate to dryness under reduced pressure. Check for homogeneity by TLC on silica gel G in 95% MeOH/5% NH4OH and visualize the spot with iodine vapour (see CHAPS above). [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.] |
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