| Identification | Back Directory | [Name]
ASCORBATE OXIDASE | [CAS]
9029-44-1 | [Synonyms]
AOX
ASO
C:C&B8
ASCORBASE
EC 1.10.3.3
EC: 1.10.3.3
Ascorbic oxidase
Vitamin C oxidase
ASCORBATE OXIDASE
Oxidase, ascorbate
L-ascorbate oxidase
Ascorbic acid oxidase
Ascorbic acid oxydase
Ascorbate dehydrogenase
Ascorbate Oxidase (ASO)
ASCORBATE OXIDASE USP/EP/BP
Ascorbate Oxidase froM plant
Ascorbate Oxidase [400 units/mg]
Ascorbate Oxidase, Cucurbita sp.
Native Zucchini Ascorbate Oxidase
L-ASCORBATE: OXYGEN OXIDOREDUCTASE
ASCORBATE OXIDASE CUCURBITA SPECIES
L-Ascorbate oxidase (from Cucumber)
Ascorbate Oxidase from microorganism
ascorbate oxidase from cucurbita sp.
Native Cucurbita sp. Ascorbate Oxidase
Native Acremonium sp. Ascorbate Oxidase
ASCORBATE OXIDASE FROM CUCURBITASPECIES
ASCORBATE OXIDASE FROM CUCURBITY SP. &
Native Cucurbita sp. L-ascorbate oxidase
ASOD, L-Ascorbate : oxygen oxidoreductase
L-Ascorbate:oxygen oxidoreductase, Ascorbase
Ascorbate oxidase from cucurbita spec., lyophil.
Ascorbate Oxidase from Cucurbita sp.,L-Ascorbate:oxygen oxidoreductase, Ascorbase | [EINECS(EC#)]
232-852-6 | [Molecular Formula]
N/A | [MDL Number]
MFCD00130560 |
| Chemical Properties | Back Directory | [storage temp. ]
2-8°C
| [solubility ]
Soluble in aqueous buffers. | [form ]
powder
| [color ]
faintly bluish
| [biological source]
plant (Cucurbita spp.) | [Specific Activity]
1,000-3,000units/mg protein |
| Hazard Information | Back Directory | [Chemical Properties]
Appearance : Light blue amorphous powder, lyophilized
Stability : Store at －20℃ (A decrease in activity of ca.20% may occur at 5℃ within 6 months.)
Molecular weight : 132,000, 140,000
Isoelectric point : between 6.0 and 7.8 , 8.2
Michaelis constant : 2.5×10－4M (Ascorbate)
Structure : 8 copper atoms per mol of enzyme
Inhibitors : cyanide, Na2S, diethyldithiocarbamate (Na)
Optimum pH : 5.6
Optimum temperature : approx. 30℃
pH Stability : pH 7.0－10.0 (25℃, 17hr)
Thermal stability : below 40℃ (pH 8.0, 30min)
Substrate specificity : This enzyme oxidizes ascorbic acid and several ascorbic derivatives.
| [Uses]
Biochemical research. | [Definition]
An enzyme found in plant tissue that acts upon ascorbic acid in the presence of oxygen to produce dehydroascorbic acid. | [General Description]
Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria. | [Biochem/physiol Actions]
Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development. |
| Questions And Answer | Back Directory | [Description]
Ascorbate oxidase (AO) is a cell wall-localized enzyme that uses oxygen to catalyse the oxidation of ascorbate (AA) to the unstable radical monodehydroascorbate (MDHA) which rapidly disproportionates to yield dehydroascorbate (DHA) and AA, and thus contributes to the regulation of the AA redox state.
Ascorbate oxidase is useful for enzymatic determination of ascorbic acid and for eliminating the interference of ascorbic acid in clinical analysis.
Effect of Various Chemicals on Ascorbate oxidase. |
|
| Company Name: |
YONG LEI SHENG WU Gold
|
| Telephone: |
029-029-81162856 |
| Website: |
www.sxylbio.com/ |
| Company Name: |
J & K SCIENTIFIC LTD.
|
| Telephone: |
18210857532 18210857532 |
| Website: |
https://www.jkchemical.com |
| Company Name: |
Sigma-Aldrich
|
| Telephone: |
021-61415566 800-8193336 |
| Website: |
https://www.sigmaaldrich.cn |
|