| Identification | Back Directory | [Name]
PULLULANASE | [CAS]
9075-68-7 | [Synonyms]
PULLULANASE
EC 3.2.1.41
Promozyme? D2
Promozyme? 400L
LIMIT DEXTRINASE
Pullulanase microbial
pullulanase from bacillus sp.
AMYLOPECTIN 6-GLUCONOHYDROLASE
pullulanase from klebsiella pneumoniae
PULLULANASE FROM BACILLUS*ACIDOPULLULYTI
pullulanase from bacillus acidopullulyticus
PULLULANASE FROM BACILLUS SP., POWDER,~1 U/MG
PromozymeTM 400L, Pullulan 6-glucano-hydrolase
PULLULANASE SUSPENSION, FROM KLEBSIELLA PNEUMONIAE
Amylopectin 6-gluconohydrolase, Limit dextrinase
Pullulanase from Klebsiella pneumoniae,Amylopectin 6-gluconohydrolase, Limit dextrinase
Pullulanase from Bacillus acidopullulyticus,Promozyme 400L, Pullulan 6-glucano-hydrolase | [EINECS(EC#)]
232-983-9 | [Molecular Formula]
NULL | [MDL Number]
MFCD00081941 |
| Chemical Properties | Back Directory | [density ]
1.25 g/mL at 25 °C(lit.)
| [vapor pressure ]
0Pa at 25℃ | [storage temp. ]
2-8°C
| [form ]
lyophilized powder
| [color ]
Light yellow to brown | [Water Solubility ]
100g/L at 25℃ | [Specific Activity]
≥1000NPUN/g | [LogP]
-1.3 at 20℃ | [CAS DataBase Reference]
9075-68-7 | [EPA Substance Registry System]
Pullulanase (9075-68-7) |
| Questions And Answer | Back Directory | [Description]
As an an amylolytic exoenzyme, Pullulanase is one of the most essential endo-type enzyme that degrades pullulan. It is found in plants and microorganisms, in which it is synthesized as an extracellular, cell surface-anchored lipoprotein by the Gram-negative bacterium such as Bacillus brevis, genus Klebsiella. Type I pullulanases specifically cleaves α-1,6-glucosidic linkages in polysaccharides, such as starch, amylopectin, glycogen, dextrin, pullulan and related oligosaccharides, whilst type II pullulanases are effective to attack α-1,6-glucosidic linkages. This enzyme is applied on a large scale in glucose and maltose syrup industries, where it is combined with the activities of other enzymes, such as β-amylase and isoamylase to manufacture maltose and oligosaccharides. Besides, it commercially serves as an industrial enzyme preparation in various reactions, such as the production of branched cyclodextrins, where pullulanase can catalyze the condensation reaction. Pullulanase is also applied as a processing aid in grain processing biotechnology for the production of ethanol and sweeteners.
| [References]
https://en.wikipedia.org/wiki/Pullulanase
http://www.sigmaaldrich.com/catalog/product/sigma/e2412?lang=en®ion=US
https://www.hindawi.com/journals/er/2012/921362/
http://www.amano-enzyme.co.jp/eng/enzyme/15.html
http://www.ssjournals.com/index.php/ijbr/article/view/610
|
| Hazard Information | Back Directory | [Chemical Properties]
Brownish-yellow fine powder or nearly white liquid. Soluble in water, practically insoluble in ethanol, chloroform, and ether. It can hydrolyze the 1,6-α-glucosidic bonds in pullulan, while amylase and α-amylase can hydrolyze the amylopectin and glycogen in dextrin into maltotriose and maltose. | [Uses]
Pullulanase is a glucanase that degrades pullulan. It is produced by the Gram-negative bacterium Klebsiella and is used for starch-debranching as well as to study polysaccharide utilization. | [Uses]
One unit will liberate 1.0 μmole of maltotriose (measured as glucose) from pullulan per min at pH 5.0 at 25°C. | [Biochem/physiol Actions]
Type I pullulanases specifically hydrolyse α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages. |
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