| Identification | More | [Name]
Naphthol AS-E | [CAS]
92-78-4 | [Synonyms]
4'-CHLORO-3-HYDROXY-2-NAPHTHANILIDE
AZOIC COUPLING COMPONENT 10
c.i. azoic coupling component 10
N-(4-CHLOROPHENYL)-3-HYDROXY-2-NAPHTHAMIDE
NAPHTHOL AS-E
NAPTHOL AS-E
AcnaNaphtholPC
DaitoGrounderE
n-(4-chlorophenyl)-3-hydroxy-2-naphthalenecarboxamid
2-Hydroxy-3-Naphthoyl-p-Chloraniline
2-Naphthalenecarboxamide-N-(4-chlorophenyl)-3-hydroxy
4′-Chlor-3-hydroxy-2-naphthanilid
Naphthol AS-E (C.I. 37510)
4''-CHLORO-3-HYDROXY-2-NAPHTHANILIDE 97.0+%(T)
C.I. 37510
Amarthol AS-E
Kiwa Grounder E
Naftol AS-E
N-(4-Chlorophenyl)-3-hydroxynaphthalene-2-carboxamide | [EINECS(EC#)]
202-189-7 | [Molecular Formula]
C17H12ClNO2 | [MDL Number]
MFCD00021639 | [Molecular Weight]
297.74 | [MOL File]
92-78-4.mol |
| Chemical Properties | Back Directory | [Melting point ]
255°C | [Boiling point ]
416.5±30.0 °C(Predicted) | [density ]
1.399±0.06 g/cm3(Predicted) | [vapor pressure ]
0Pa at 25℃ | [storage temp. ]
Sealed in dry,Room Temperature | [solubility ]
soluble in Dimethylformamide | [Colour Index ]
37510 | [form ]
Beige solid | [pka]
8.69±0.40(Predicted) | [color ]
White to Light yellow to Dark green | [LogP]
2.97 at 23℃ and pH7.2 | [CAS DataBase Reference]
92-78-4(CAS DataBase Reference) | [EPA Substance Registry System]
92-78-4(EPA Substance) |
| Hazard Information | Back Directory | [Uses]
Naphthol AS-E is mainly used for dyeing cotton, and also for dyeing viscose fiber and silk. It is often used in combination with blue base or blue salt to dye dark blue and navy blue. | [Preparation]
4-Chlorobenzenamine and 3-Hydroxy-2-naphthoic acid condensation. | [General Description]
A cell-permeable naphthamide compound that effectively blocks the interaction between the KIX domain of CBP and the KID domain of CREB in HEK293T-based and cell-free Renilla luciferase (RLuc) complementation assays (IC50 = 2.26 and 2.9 M, respectively) by directly binding to CBP′s KIX domain (Kd ~8.6 M using a recombinant KIX). Shown to inhibit CRE- (cAMP response element) dependent transcription activity induced by forskolin in a HEK293T-based RLuc reporter assay (IC50<2.5 M), while exhibiting little effect against the KID-independent CREB-VP16 transcription activity. Also reported to inhibit the enzymatic activity of Firefly luciferase (IC50 ~1 M), but not that of RLuc. | [Biological Activity]
NaphtholAS-E is a potent, cell-permeable inhibitor of the KIX-KID interaction. NaphtholAS-E directly binds to the KIX domain of CBP (Kd: 8.6μM) and blocks the interaction between the KIX and KID domains (IC50: 2.26μM). NaphtholAS-E can be used in cancer research. | [Synthesis]
Naphthol AS-E is synthesized with 2,3-acid and p-chloroaniline as raw materials and chlorobenzene as solvent. First, 2,3-acid is made into sodium salt, and then condensed with p-chloroaniline in the presence of PCl3, and the finished product is obtained by neutralization, distillation, filtration and drying. | [in vitro]
CREB (cyclic AMP-response element-binding protein) is a downstream transcription factor of a multitude of signaling pathways emanating from receptor tyrosine kinases or G-protein coupled receptors.CREB can not be activated until it is phosphorylated at Ser133 and its subsequent binding to CREB-binding protein (CBP) through the kinase-inducible domain (KID) in CREB and KID-interacting (KIX) domain in CBP.In a cell-based CREB Renilla luciferase reporter assay, Naphthol AS-E inhibits CREB-mediated gene transcription with an IC 50 of 2.29 μM. In HEK293T-based complementation assay, Naphthol AS-E dose-dependently inhibited Renilla luciferase activity with an IC 50 of 2.9 μM by directly binding to CBP's KIX domain (K d ~8.6 μM using a recombinant KIX).Naphthol AS-E exhibits low μM activity in inhibiting the proliferation of all these cancer cells, which is consistent with its cellular CREB inhibition potency. The average GI 50 values for A549, MCF-7, MDA-MB-231 and MDA-MB-468 are approximately 2.9μM, 2.81μM, 2.35μM and 1.46μM, respectively.Naphthol AS-E (2.5 μM-10 μM; 48 hours) decreases the expression of anti-apoptotic protein Bcl-2. The expression of VEGF is also decreased. | [Properties and Applications]
light yellow microstrip red uniform powder, melting point for 258 ~ 259 ℃. Insoluble in water solution and sodium carbonate, soluble in adjacent two chlorobenzene, ethanol and glacial acetic acid. Sodium hydroxide solution for yellow in the liquid. Sensitive to air. Mainly used for cotton fabrics, viscose and silk dyeing, general is not used for printing, and can be used as organic pigments intermediate. This product to cotton affinity is higher, coupled ability medium. |
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