200626-61-5
200626-61-5 结构式
基本信息
ITSA-1(ITSA1)
IFLAB-BB F0451-3184
1-(2,4-Dichlorobenzoyl)-1H-benzotriazole
BENZOTRIAZOL-1-YL-(2,4-DICHLORO-PHENYL)-METHANONE
Methanone, 1H-benzotriazol-1-yl(2,4-dichlorophenyl)-
常见问题列表
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HDAC
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ITSA1 (50 μM; A549 cells) treatment serves to revert the TSA-arrested population to a normal cell cycle distribution. ITSA1 is also able to effect cell cycle rescue over longer duration.
ITSA1 (50 μM; 5 hours; A549 cells) treatment reduces the number of apoptosis in TSA-treated cells.
ITSA1 (50 μM; 2 hours; A549 and murine ES cells cells) treatment suppresses TSA-induced histone acetylation. Importantly, suppression of acetylation levels is only observable when ITSA1 is added concurrent with or post TSA treatment.
ITSA1 (50 μM; 30 minutes; murine ES cells cells) suppresses TSA-activated transcription in murine ES cells.
Cell Cycle Analysis
| Cell Line: | Murine ES cells |
| Concentration: | 50 μM |
| Incubation Time: | |
| Result: | Served to revert the TSA-arrested population to a normal cell cycle distribution. |
Apoptosis Analysis
| Cell Line: | A549 cells |
| Concentration: | 50 μM |
| Incubation Time: | 5 hours |
| Result: | Reduced the number of apoptosis. |
Western Blot Analysis
| Cell Line: | A549 and murine ES cells |
| Concentration: | 50 μM |
| Incubation Time: | 2 hours |
| Result: | Reduced histone acetylation to the baseline level. |
RT-PCR
| Cell Line: | Murine ES cells |
| Concentration: | 50 μM |
| Incubation Time: | 30 minutes |
| Result: | Suppressed TSA-activated transcription. |
ITSA-1 (0.5 mg/kg; intraperitoneal injection; 3 times/week; for 8 weeks; CBS +/− mice) treatment balances deacetylation activity and suppresses IL-6 and TNF-α expression and thereby attenuated histone acetylationdependent infammatory signaling.
| Animal Model: | CBS +/− mice |
| Dosage: | 0.5 mg/kg |
| Administration: | Intraperitoneal injection; 3 times/week; for 8 weeks |
| Result: | Balanced deacetylation activity and suppressed IL-6 and TNF-α expression. |