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Fluorescein isothiocyanate isomer I

Fluorescein isothiocyanate isomer I
Fluorescein isothiocyanate isomer I
CAS No.
3326-32-7
Chemical Name:
Fluorescein isothiocyanate isomer I
Synonyms
to-;FITC;5-FITC;FITC-I;FITC-I)F;FITC ISOMER;FITC-CELITE(R);FITC, ISOMER I;FITC 'ISOMER I';5-FITC (isomer I)
CBNumber:
CB0173162
Molecular Formula:
C21H11NO5S
Formula Weight:
389.38
MOL File:
3326-32-7.mol

Fluorescein isothiocyanate isomer I Properties

Melting point:
>360 °C(lit.)
Density 
1.3423 (rough estimate)
refractive index 
1.5060 (estimate)
storage temp. 
2-8°C
solubility 
Slightly soluble in DMSO, ethanol, methanol DMF and acetone
pka
2.2, 4.4, 6.7(at 25℃)
form 
powder
color 
White
PH Range
Weak green uorescence (6.0) to strong green uorescence (7.2)
Water Solubility 
practically insoluble
Sensitive 
Moisture Sensitive
λmax
490nm
BRN 
1407295
Stability:
Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids.
Major Application
Magnetic nanowires, immunoassays, analyzing proteins, identifying chromosomes, Salmonella mosomes, diagnosis of cancer, kidney diseases, detecting pathogens, genes, Salmonella cells, toxoplasmosis, enzyme-mediated nucleic acid cleavage, surface molecules on colorectal cancers, treating cancer, chronic lymphocytic leukemia
Biological Applications
Analyzing sugar chain; detecting iodide/iodate or total iodine; detecting/imaging thiols; detecting/quantifying biotin derivatives;16 diagnosing classical Hodgkin lymphoma (CHL) in lymph nodes; cellulose nanocrystals for bioimaging applications; treating neuroinflammation; treating/diagnosing cancer; PEO-PCL-PEO triblock copolymers for topical delivery; polymersomes in biomedical or cosmetic applications;apoptosis assay; as a substrate for measuring heparanase activity, human immunodeficiency virus (HIV-1) proteinase activity, kinase activity, phosphatase activity, β-lactamase activity, transglutaminase (TG2) activity; implantable drug-delivery devicess
InChIKey
MHMNJMPURVTYEJ-UHFFFAOYSA-N
CAS DataBase Reference
3326-32-7(CAS DataBase Reference)
EPA Substance Registry System
Spiro[isobenzofuran- 1(3H),9'-[9H]xanthen]- 3-one, 3',6'-dihydroxy- 5-isothiocyanato-(3326-32-7)
SAFETY
  • Risk and Safety Statements
  • Hazard and Precautionary Statements (GHS)
Hazard Codes  Xn,Xi
Risk Statements  42/43-36/37/38-22
Safety Statements  22-45-36/37/39-26-36/37-36
WGK Germany  3
10
TSCA  Yes
HS Code  29329990
Symbol(GHS):
Signal word: Danger
Hazard statements:
Code Hazard statements Hazard class Category Signal word Pictogram P-Codes
H315 Causes skin irritation Skin corrosion/irritation Category 2 Warning P264, P280, P302+P352, P321,P332+P313, P362
H317 May cause an allergic skin reaction Sensitisation, Skin Category 1 Warning P261, P272, P280, P302+P352,P333+P313, P321, P363, P501
H319 Causes serious eye irritation Serious eye damage/eye irritation Category 2A Warning P264, P280, P305+P351+P338,P337+P313P
H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled Sensitisation, respiratory Category 1 Danger P261, P285, P304+P341, P342+P311,P501
H335 May cause respiratory irritation Specific target organ toxicity, single exposure;Respiratory tract irritation Category 3 Warning
Precautionary statements:
P260 Do not breathe dust/fume/gas/mist/vapours/spray.
P261 Avoid breathing dust/fume/gas/mist/vapours/spray.
P262 Do not get in eyes, on skin, or on clothing.
P264 Wash hands thoroughly after handling.
P264 Wash skin thouroughly after handling.
P270 Do not eat, drink or smoke when using this product.
P271 Use only outdoors or in a well-ventilated area.
P272 Contaminated work clothing should not be allowed out of the workplace.
P280 Wear protective gloves/protective clothing/eye protection/face protection.
P284 Wear respiratory protection.
P309 IF exposed or if you feel unwell:
P310 Immediately call a POISON CENTER or doctor/physician.
P342+P311 IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P405 Store locked up.
P403+P233 Store in a well-ventilated place. Keep container tightly closed.
P501 Dispose of contents/container to..…

Fluorescein isothiocyanate isomer I price More Price(16)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 34321-M FITC, Isomer I 3326-32-7 1gm-m $247 2018-11-20 Buy
Sigma-Aldrich 46951 Fluorescein isothiocyanate isomer I ≥97.5% (HPLC) 3326-32-7 250mg-f $454 2018-11-20 Buy
TCI Chemical F0026 Fluorescein 5-Isothiocyanate (isomer I) >97.0%(HPLC)(T) 3326-32-7 1g $209 2018-11-22 Buy
TCI Chemical F0026 Fluorescein 5-Isothiocyanate (isomer I) >97.0%(HPLC)(T) 3326-32-7 100mg $35 2018-11-22 Buy
Alfa Aesar L09319 Fluorescein isothiocyanate, isomer 1, 95% 3326-32-7 50mg $28.5 2018-11-13 Buy

Fluorescein isothiocyanate isomer I Chemical Properties,Uses,Production

Chemical Properties

Orange-yellow cryst.

Uses

Fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines. Used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.

Uses

Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens

Definition

ChEBI: The 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Purification Methods

It is made from the pure 5-amino isomer. Purify it by dissolving it in boiling Me2CO, filtering and adding pet ether (b 60-70o) until it becomes turbid. If an oil separates, then decant it and add more pet ether to the supernatant and cool. Orange-yellow crystals separate, collect and dry them in vacuo. It should give one spot on TLC (silica gel) in EtOAc/pyridine/AcOH (50:1:1) and in Me2NCHO/CHCl3/28% NH4OH (10:5:4). IR (Me2SO): 2110 (NCS) and 1760 (C=O) cm-1. The max 1HNMR spectra in Me2CO-d6 of the 5-and 6-isomers are distinctly different for the protons in the *benzene ring; the UV in phosphate buffer pH 8.0 shows a at ~490nm. [Sinsheimer et al. Anal Biochem 57 2271974, McKinney et al. Anal Biochem 7 74 1964, Beilstein 19 III/IV 4337.]

Fluorescein isothiocyanate isomer I Preparation Products And Raw materials

Raw materials

Preparation Products


Fluorescein isothiocyanate isomer I Suppliers

Global( 198)Suppliers
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View Lastest Price from Fluorescein isothiocyanate isomer I manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2018-12-21 Fluorescein isothiocyanate isomer I
3326-32-7
US $1.00 / kg 1kg 98% 100KG career henan chemical co

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