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Fluorescein isothiocyanate isomer I

CAS No.
3326-32-7
Chemical Name:
Fluorescein isothiocyanate isomer I
Synonyms
FITC;FITC;FITC;5-FITC;Fluorescein isothiocyanate isomer;5-FLUORESCEIN ISOTHIOCYANATE;Fluorescein isothiocyanate isomer I, tech grade;FITC-I;FLUORESCEIN 5-ISOTHIOCYANATE;Fluorescein isothiocyanate I
CBNumber:
CB0173162
Molecular Formula:
C21H11NO5S
Molecular Weight:
389.38
MDL Number:
MFCD00005063
MOL File:
3326-32-7.mol
MSDS File:
SDS
Last updated:2023-11-13 19:05:53

Fluorescein isothiocyanate isomer I Properties

Melting point >360 °C(lit.)
Boiling point 708.6±60.0 °C(Predicted)
Density 1.3423 (rough estimate)
refractive index 1.5060 (estimate)
storage temp. 2-8°C
solubility Slightly soluble in DMSO, ethanol, methanol DMF and acetone
form powder
pka 2.2, 4.4, 6.7(at 25℃)
color White
PH Range Weak green uorescence (6.0) to strong green uorescence (7.2)
Water Solubility practically insoluble
Sensitive Moisture Sensitive
λmax 490nm
BRN 1407295
Stability Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids.
Major Application Magnetic nanowires, immunoassays, analyzing proteins, identifying chromosomes, Salmonella mosomes, diagnosis of cancer, kidney diseases, detecting pathogens, genes, Salmonella cells, toxoplasmosis, enzyme-mediated nucleic acid cleavage, surface molecules on colorectal cancers, treating cancer, chronic lymphocytic leukemia
Biological Applications Analyzing sugar chain; detecting iodide/iodate or total iodine; detecting/imaging thiols; detecting/quantifying biotin derivatives;16 diagnosing classical Hodgkin lymphoma (CHL) in lymph nodes; cellulose nanocrystals for bioimaging applications; treating neuroinflammation; treating/diagnosing cancer; PEO-PCL-PEO triblock copolymers for topical delivery; polymersomes in biomedical or cosmetic applications;apoptosis assay; as a substrate for measuring heparanase activity, human immunodeficiency virus (HIV-1) proteinase activity, kinase activity, phosphatase activity, β-lactamase activity, transglutaminase (TG2) activity; implantable drug-delivery devicess
InChIKey MHMNJMPURVTYEJ-UHFFFAOYSA-N
CAS DataBase Reference 3326-32-7(CAS DataBase Reference)
FDA UNII I223NX31W9
EPA Substance Registry System Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato- (3326-32-7)

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS08
Signal word  Danger
Hazard statements  H317-H334
Precautionary statements  P261-P272-P280-P284-P302+P352-P333+P313
Hazard Codes  Xn,Xi
Risk Statements  42/43-36/37/38-22
Safety Statements  22-45-36/37/39-26-36/37-36
WGK Germany  3
10
TSCA  Yes
HS Code  29329990
NFPA 704
0
2 0

Fluorescein isothiocyanate isomer I price More Price(46)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 46951 Fluorescein isothiocyanate isomer I ≥97.5% (HPLC) 3326-32-7 250mg $620 2024-03-01 Buy
TCI Chemical F0026 Fluorescein 5-Isothiocyanate (isomer I) >97.0%(HPLC)(T) 3326-32-7 1g $388 2024-03-01 Buy
TCI Chemical F0026 Fluorescein 5-Isothiocyanate (isomer I) >97.0%(HPLC)(T) 3326-32-7 100mg $61 2024-03-01 Buy
Alfa Aesar L09319 Fluorescein isothiocyanate, isomer 1, 95% 3326-32-7 50mg $38.8 2024-03-01 Buy
Alfa Aesar L09319 Fluorescein isothiocyanate, isomer 1, 95% 3326-32-7 1g $306 2024-03-01 Buy
Product number Packaging Price Buy
46951 250mg $620 Buy
F0026 1g $388 Buy
F0026 100mg $61 Buy
L09319 50mg $38.8 Buy
L09319 1g $306 Buy

Fluorescein isothiocyanate isomer I Chemical Properties,Uses,Production

Description

Fluorescein 5-isothiocyanate (5-FITC) is an isomer of fluorescein isothiocyanate (FITC), which is commonly used as a mixture of the 5- and 6-isothiocyanate isomers. It reacts with amine and thiol groups to form conjugates with proteins, lipids, and other molecules for detection by a variety of fluorescent-based applications. 5-FITC displays excitation/emission maxima of 494/519 nm, respectively.

Chemical Properties

Fluorescein isothiocyanate(3326-32-7) is an orange-yellow crystal that is hygroscopic. It can be combined with various antibody proteins, and the combined antibody does not lose its specificity for binding to a certain antigen, and still has strong green fluorescence in alkaline solution, precipitates out after adding acid, and the fluorescence disappears. It is slightly soluble in acetone and ether and petroleum ether.

Uses

Fluorescein isothiocyanate isomer I has been used for the staining of conidia. It is a kind of fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines and it is also used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.

Application

Fluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of pathogens in fluorescent antibody technique. It is also used as a reagent for microsequencing of proteins and peptides (HPLC). On celite it is used for the labeling of fibrinogen.

Definition

ChEBI: Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

General Description

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.

storage

Store at -20°C

Purification Methods

It is made from the pure 5-amino isomer. Purify it by dissolving it in boiling Me2CO, filtering and adding pet ether (b 60-70o) until it becomes turbid. If an oil separates, then decant it and add more pet ether to the supernatant and cool. Orange-yellow crystals separate, collect and dry them in vacuo. It should give one spot on TLC (silica gel) in EtOAc/pyridine/AcOH (50:1:1) and in Me2NCHO/CHCl3/28% NH4OH (10:5:4). IR (Me2SO): 2110 (NCS) and 1760 (C=O) cm-1. The max 1HNMR spectra in Me2CO-d6 of the 5-and 6-isomers are distinctly different for the protons in the *benzene ring; the UV in phosphate buffer pH 8.0 shows a at ~490nm. [Sinsheimer et al. Anal Biochem 57 2271974, McKinney et al. Anal Biochem 7 74 1964, Beilstein 19 III/IV 4337.]

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View Lastest Price from Fluorescein isothiocyanate isomer I manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Fluorescein isothiocyanate isomer I pictures 2023-11-13 Fluorescein isothiocyanate isomer I
3326-32-7
US $0.00 / KG 1KG 99% 50000KG/month Hebei Mojin Biotechnology Co., Ltd
Fluorescein isothiocyanate isomer I pictures 2023-09-26 Fluorescein isothiocyanate isomer I
3326-32-7
US $30.00-9.00 / kg 1kg 0.99 20 tons Hebei Yanxi Chemical Co., Ltd.
Fluorescein isothiocyanate isomer I pictures 2023-09-06 Fluorescein isothiocyanate isomer I
3326-32-7
US $0.00-0.00 / KG 1KG 99% 500000kg Hebei Guanlang Biotechnology Co., Ltd.
FITC-CELITE(R) FITC ISOMER FITC 'ISOMER I' FITC, ISOMER I FLUORESCEIN ISOMER I ISOTHIOCYANATE FLUORESCEIN ISOTHIOCYANATE, ISOMER 1 FLUORESCEIN ISOTHIOCYANATE ISOMER I FLUORESCEIN ISOTHIOCYANATE ISOMER I-CELITE(R) FLUORESCEIN 5(6)-ISOTHIOCYANATE FLUORESCEIN 5-ISOTHIOCYANATE, ISOMER 1 FLUORESCEIN-5-ISOTHIOCYANATE ISOMER I 5-FLUORESCEIN ISOTHIOCYANATEISOMER 5-FLUORESCEIN ISOTHIOCYANATE, ISOMER I 5-ISOTHIOCYANATOFLUORESCEIN SPECTRACOMP(TM) FITC PARTICLES 2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-5-ISOTHIOCYANATO-BENZOIC ACID Spiroisobenzofuran-1(3H),9-9Hxanthen-3-one, 3,6-dihydroxy-5-isothiocyanato- Fluorescein isothiocyanate,Isomer I(FITC) Fluorescein isothiocyanate isomer I,FITC, Fluorescein 5-isothiocyanate Fluorescein isothiocyanate, tech. 85% 3,6-Dihydroxy-3'-oxospiro[9H-xanthene-9,1'(3'H)-isobenzofuran]-5'-yl isothiocyanate 3',6'-Dihydroxy-5-isothiocyanato-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one FLUORESCEIN ISOTHIOCYANTE ISOMER I ON CELITE extrapure FITC, Fluorescein 5-isothiocyanate Fluorescein isothiocyanate isomer i, 90%, pure 5-Isothionatofluorescein Fluorescein isothiocyanate isomer I,(cert) Fluoresceinisothiocyanate, Isomer I >98% Fluorescein isothiocyanate isoMer I, pure, 90% 250MG Fluorescein isothiocyanate isoMer I, pure, 90% 5GR Fluorescein Isothiocyanate 5-Isothiocyanatofluorescein 5-FITC FLUORESCEIN-5-ISOTHIOCYANATE FOR BIOCHEM Fluorescein-5-isothiocyanate (FITC-I) FITC-I)F FLUORESCEIN ISOTHIOCY 5-FITC (isomer I) 5-Isothiocyanatofluorescein (isomer I) Fluorescein-5-isothiocyanate (FITC) for biochemistry and for fluorescence microscopy 3',6'-dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one Fluorescein-5-isothiocyanate (fitc) Fluorescein, 5-isothiocyanato- 5-isothiocyanato Uorescein Fluoroscein-5-isothiocyanate 9’-[9H]xanthen]-3-one,3’,6’-dihydroxy-5-isothiocyanato-Spiro[isobenzofuran-1(3H) spiro[isobenzofuran-1(3h),9’-[9h]xanthen]-3-one,3’,6’-dihydroxy-5-isothiocyana to- FLUORESCEIN ISOTHIOCYANATE ISOMER 1 95+% Fitc(FluoresceinIsothiocyanate),IsomerI Fluorescein Isothiosyanate Fluorescein isothiocyanate isomer I, pure, 90% 5-Fluorescein Isothiocyanate isomer 1 FLUORESCEIN ISOTHIOCYANATE ISOMER I*MINI MUM 90% FLUORESCEIN ISOTHIOCYANATE ISOMER I, FOR FLUORESCENCE FLUORESCEIN ISOTHIOCYANATE, ISOMER I, 90 % FLUORESCEIN ISOTHIOCYANATE ISOMER I*APPR OX. 98% 5-FITC [Fluorescein-5-isothiocyanate 3',6'-dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9'-xanthene]-1-one Fluorescein isothiocyanate,10%