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Fluorescein isothiocyanate isomer I

Fluorescein isothiocyanate isomer I
Fluorescein isothiocyanate isomer I
Chemical Name:
Fluorescein isothiocyanate isomer I
Molecular Formula:
Formula Weight:
MOL File:

Fluorescein isothiocyanate isomer I Properties

Melting point:
>360 °C(lit.)
1.3423 (rough estimate)
refractive index 
1.5060 (estimate)
storage temp. 
Slightly soluble in DMSO, ethanol, methanol DMF and acetone
2.2, 4.4, 6.7(at 25℃)
PH Range
Weak green uorescence (6.0) to strong green uorescence (7.2)
Water Solubility 
practically insoluble
Moisture Sensitive
Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids.
Major Application
Magnetic nanowires, immunoassays, analyzing proteins, identifying chromosomes, Salmonella mosomes, diagnosis of cancer, kidney diseases, detecting pathogens, genes, Salmonella cells, toxoplasmosis, enzyme-mediated nucleic acid cleavage, surface molecules on colorectal cancers, treating cancer, chronic lymphocytic leukemia
Biological Applications
Analyzing sugar chain; detecting iodide/iodate or total iodine; detecting/imaging thiols; detecting/quantifying biotin derivatives;16 diagnosing classical Hodgkin lymphoma (CHL) in lymph nodes; cellulose nanocrystals for bioimaging applications; treating neuroinflammation; treating/diagnosing cancer; PEO-PCL-PEO triblock copolymers for topical delivery; polymersomes in biomedical or cosmetic applications;apoptosis assay; as a substrate for measuring heparanase activity, human immunodeficiency virus (HIV-1) proteinase activity, kinase activity, phosphatase activity, β-lactamase activity, transglutaminase (TG2) activity; implantable drug-delivery devicess
CAS DataBase Reference
3326-32-7(CAS DataBase Reference)
EPA Substance Registry System
Spiro[isobenzofuran- 1(3H),9'-[9H]xanthen]- 3-one, 3',6'-dihydroxy- 5-isothiocyanato-(3326-32-7)
  • Risk and Safety Statements
  • Hazard and Precautionary Statements (GHS)
Hazard Codes  Xn,Xi
Risk Statements  42/43-36/37/38-22
Safety Statements  22-45-36/37/39-26-36/37-36
WGK Germany  3
HS Code  29329990
Signal word: Danger
Hazard statements:
Code Hazard statements Hazard class Category Signal word Pictogram P-Codes
H315 Causes skin irritation Skin corrosion/irritation Category 2 Warning P264, P280, P302+P352, P321,P332+P313, P362
H317 May cause an allergic skin reaction Sensitisation, Skin Category 1 Warning P261, P272, P280, P302+P352,P333+P313, P321, P363, P501
H319 Causes serious eye irritation Serious eye damage/eye irritation Category 2A Warning P264, P280, P305+P351+P338,P337+P313P
H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled Sensitisation, respiratory Category 1 Danger P261, P285, P304+P341, P342+P311,P501
H335 May cause respiratory irritation Specific target organ toxicity, single exposure;Respiratory tract irritation Category 3 Warning
Precautionary statements:
P260 Do not breathe dust/fume/gas/mist/vapours/spray.
P261 Avoid breathing dust/fume/gas/mist/vapours/spray.
P262 Do not get in eyes, on skin, or on clothing.
P264 Wash hands thoroughly after handling.
P264 Wash skin thouroughly after handling.
P270 Do not eat, drink or smoke when using this product.
P271 Use only outdoors or in a well-ventilated area.
P272 Contaminated work clothing should not be allowed out of the workplace.
P280 Wear protective gloves/protective clothing/eye protection/face protection.
P284 Wear respiratory protection.
P309 IF exposed or if you feel unwell:
P310 Immediately call a POISON CENTER or doctor/physician.
P342+P311 IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P405 Store locked up.
P403+P233 Store in a well-ventilated place. Keep container tightly closed.
P501 Dispose of contents/container to..…

Fluorescein isothiocyanate isomer I price More Price(16)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 34321-M FITC, Isomer I 3326-32-7 1gm-m $247 2018-11-20 Buy
Sigma-Aldrich 46951 Fluorescein isothiocyanate isomer I ≥97.5% (HPLC) 3326-32-7 250mg-f $454 2018-11-20 Buy
TCI Chemical F0026 Fluorescein 5-Isothiocyanate (isomer I) >97.0%(HPLC)(T) 3326-32-7 1g $209 2018-11-22 Buy
TCI Chemical F0026 Fluorescein 5-Isothiocyanate (isomer I) >97.0%(HPLC)(T) 3326-32-7 100mg $35 2018-11-22 Buy
Alfa Aesar L09319 Fluorescein isothiocyanate, isomer 1, 95% 3326-32-7 50mg $28.5 2018-11-13 Buy

Fluorescein isothiocyanate isomer I Chemical Properties,Uses,Production

Chemical Properties

Orange-yellow cryst.


Fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines. Used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.


Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens


ChEBI: The 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Purification Methods

It is made from the pure 5-amino isomer. Purify it by dissolving it in boiling Me2CO, filtering and adding pet ether (b 60-70o) until it becomes turbid. If an oil separates, then decant it and add more pet ether to the supernatant and cool. Orange-yellow crystals separate, collect and dry them in vacuo. It should give one spot on TLC (silica gel) in EtOAc/pyridine/AcOH (50:1:1) and in Me2NCHO/CHCl3/28% NH4OH (10:5:4). IR (Me2SO): 2110 (NCS) and 1760 (C=O) cm-1. The max 1HNMR spectra in Me2CO-d6 of the 5-and 6-isomers are distinctly different for the protons in the *benzene ring; the UV in phosphate buffer pH 8.0 shows a at ~490nm. [Sinsheimer et al. Anal Biochem 57 2271974, McKinney et al. Anal Biochem 7 74 1964, Beilstein 19 III/IV 4337.]

Fluorescein isothiocyanate isomer I Preparation Products And Raw materials

Raw materials

Preparation Products

Fluorescein isothiocyanate isomer I Suppliers

Global( 198)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Springchem New Material Technology Co.,Limited
86-21-51752789 China 929 57
Mainchem Co., Ltd.
+86-0592-6210733 CHINA 32651 55
Hangzhou FandaChem Co.,Ltd.
0086 158 5814 5714 (Mobile; WhatsApp; Telegram)
+86-571-56059825 CHINA 2097 55
Nanjing ChemLin Chemical Industry Co., Ltd.
025-83697070; CHINA 3015 60
Hubei Jusheng Technology Co.,Ltd.
86-155-27864001 CHINA 18004 58
career henan chemical co
+86-371-86658258 CHINA 19990 58
Okeanos Tech Co,. Ltd. (+86) 10-6265-1721
(+86) 10-6265-1521-808 China 457 60
Nanjing UltraPure Biotech Co., Ltd. 025-81682922
025-85860962 China 206 58
Dezhou LonWel Pharmaceutical Technology Co., Ltd. 13761310616
QQ: 39324283 China 1856 58
Nanjing Dulai Biotechnology Co., Ltd. 025-84699383 84699415 13382013214 13382013204
025-52453057 China 905 55

View Lastest Price from Fluorescein isothiocyanate isomer I manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2018-12-21 Fluorescein isothiocyanate isomer I
US $1.00 / kg 1kg 98% 100KG career henan chemical co

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