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Guanidine thiocyanate

Uses Biochem/physiol Actions Description Uses Toxicity evaluation
Guanidine thiocyanate
Guanidine thiocyanate structure
Chemical Name:
Guanidine thiocyanate
GTC;GuSCN;GdmSCN;usafek-705;GUANIDIN THIOCY;GUANIDINE THIOCY;Guanidine Research;GUANIDINE RHODANIDE;Guandine thiocyanat;uandine thiocyanate
Molecular Formula:
Formula Weight:
MOL File:

Guanidine thiocyanate Properties

Melting point:
120-122 °C(lit.)
1.103 g/mL at 20 °C
refractive index 
n20/D 1.482
storage temp. 
Store at RT.
H2O: 6 M at 20 °C, clear, colorless
Crystals or Crystalline Powder
Specific Gravity
White or colorless
4.8-6.0 (1420g/l, H2O, 20℃)(saturated solution)
PH Range
4.8 - 6.0
Water Solubility 
Soluble in water and ethanol
Light Sensitive
Stability Stable, but light sensitive. Incompatible with acids (contact releases very toxic gas), strong oxidising agents.
CAS DataBase Reference
593-84-0(CAS DataBase Reference)
EPA Substance Registry System
Thiocyanic acid, compd. with guanidine (1:1) (593-84-0)
  • Risk and Safety Statements
Signal word  Danger
Hazard statements  H302+H332-H315-H319-H335-H302-H312-H332-H402-H302+H312+H332-H314-H412
Precautionary statements  P280h-P301+P312a-P304+P340-P321-P501a-P261-P264-P270-P271-P273-P302+P352+P332+P313+P362+P364-P304+P340+P312-P305+P351+P338+P337+P313-P403+P233-P405-P501-P260-P280-P301+P312+P330-P303+P361+P353-P304+P340+P310-P305+P351+P338
Hazard Codes  Xn,T,C
Risk Statements  20/21/22-32-52/53-36/37/38-34
Safety Statements  36/37-61-13-36-26-45-36/37/39
RIDADR  1759
WGK Germany  2
RTECS  XL1225000
HazardClass  8
PackingGroup  III
HS Code  29252000

Guanidine thiocyanate price More Price(30)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 368975 Guanidine Thiocyanate Powerful protein denaturant. Absorbance (1.7 M, H2O, 280 nm):0.30. 593-84-0 100gm $93.6 2020-08-18 Buy
Sigma-Aldrich 368975 Guanidine Thiocyanate Powerful protein denaturant. Absorbance (1.7 M, H2O, 280 nm):0.30. 593-84-0 500gm $389 2020-08-18 Buy
TCI Chemical G0230 Guanidine Thiocyanate >99.0%(T) 593-84-0 25g $13 2020-06-24 Buy
TCI Chemical G0230 Guanidine Thiocyanate >99.0%(T) 593-84-0 500g $159 2020-06-24 Buy
Alfa Aesar B21250 Guanidine thiocyanate, 99% 593-84-0 100g $66.8 2020-06-24 Buy

Guanidine thiocyanate Chemical Properties,Uses,Production


Guanidine thiocyanate has been used:
• for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation
• as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay

Biochem/physiol Actions

Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction.


Guanidine thiocyanate is one of the cheapest and easiest to prepare of the guanidine salts. Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents. It is provided in one bottle containing 500 g. It is recommended for isolation of RNA, especially for tissues such as pancreas with high levels of RNase activity. In solution, it is known as guanidinium thiocyanate. Guanidine Thiocyanate is thoroughly tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.


It is mainly used for biomedicine, chemical reagents.
Guanidine thiocyanate is a potent protein denaturant (stronger than guanidine HCl) often used in the isolation of intact ribonucleic acid to eliminate RNase activity. RNase can recover activity after boiling, but is irreversibly inactivated in a 4 M solution of guanidine thiocyanate. Such solutions, to which the reducing agent b-mercaptoethanol is often added, are used to inactivate RNAse when isolating RNA from tissues that are rich in RNase, such as liver. Total nuclear and cytoplasmic RNA may be isolated this way. A protocol for extracting RNA with guanidine isothiocyanate has been published.
In the presence of guanidine thiocyanate, proteins dissolve readily, cellular structures disintegrate and nucleoproteins dissociate from nucleic acids, as protein secondary structure is lost.

Toxicity evaluation

Guanidine thiocyanate is used commercially as a disinfectant and a general protein denaturant but its more common use is in the extraction of DNA and RNA in molecular biology. Нere are no reports of guanidine thiocyanate toxicity from oral ingestion. Toxicity of diوٴerent thiocyanate compounds other than guanidine thiocyanate has been described. Potassium thiocyanate was used over 50 years ago for the treatment of hypertension and its toxicity appears to cause a neurological disorder consisting of generalized weakness, delirium and a decrease level of consciousness. Reports of thiocyanate toxicity from nitroprusside use for hypertension in the setting of renal impairment/failure have also been reported as thiocyanate is renally cleared. In a fatal deliberate ingestion of a herbicide which contained ammonium thiocyanate and aminotriazole, a 54 year old man presented with coma and cardiovascular collapse although the relative contributions of the two herbicides to the clinical toxicity is unknown.

Chemical Properties

White cryst.powder


Potent protein denaturant used in isolation of intact DNA, RNA.



Guanidine thiocyanate Preparation Products And Raw materials

Raw materials

Preparation Products

Guanidine thiocyanate Suppliers

Global( 351)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Jiangsu Aikon Biooharmaceutical R&D CO.Ltd
-- China 5 12
BOC Sciences
1-631-619-7922 1-631-619-7922
1-631-614-7828 United States 20039 58
Hefei TNJ Chemical Industry Co.,Ltd.
86-0551-65418684 18949823763
86-0551-65418684 China 2799 55
86-18523575427 CHINA 47486 58
Henan Tianfu Chemical Co.,Ltd.
0371-55170693 CHINA 22617 55
Jinan Qinmu Fine Chemical Co.,Ltd.
18660799346 18363098612
+86 531 8316 6650 CHINA 880 58
Jinan Finer Chemical Co., Ltd
+86-531-88989539 CHINA 997 58
WinWin Chemical CO., Limited
15325081899 0086-577-64498589
0086-577-56994596 CHINA 10960 58
Henan DaKen Chemical CO.,LTD.
+86-371-55531817 CHINA 21711 58
Hangzhou FandaChem Co.,Ltd.
0086 158 5814 5714 (Mobile
+86-571-56059825 CHINA 8463 55

View Lastest Price from Guanidine thiocyanate manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2020-11-10 Guanidine thiocyanate
US $0.00 / KG 1KG 99% 200mt Jinan Finer Chemical Co., Ltd
2021-02-18 Guanidine thiocyanate
US $10.00 / g 1g 99.95% 20tons Hebei Minshang Biotechnology Co., Ltd
2020-12-11 Guanidine thiocyanate
US $10.00 / KG 100IU 99% 100 Hebei Henghe Import and Export Trading Co. LTD

Guanidine thiocyanate Spectrum

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