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3,3'-Diaminobenzidine

3,3'-Diaminobenzidine Structure
3,3'-Diaminobenzidine structure
CAS No.
91-95-2
Chemical Name:
3,3'-Diaminobenzidine
Synonyms
DAB;DIAMINOBENZIDINE;[1,1'-biphenyl]-3,3',4,4'-tetraaMine;3,3';DAB BUFFER;JACS-91-95-2;TETRAAMINOBIPHENYL;TIMTEC-BB SBB008427;3'-DiaMinobenzidine;3,3'-Diaminobenzidin
CBNumber:
CB0366173
Molecular Formula:
C12H14N4
Molecular Weight:
214.27
MDL Number:
MFCD00007725
MOL File:
91-95-2.mol
MSDS File:
SDS
TDS File:
TDS
Last updated:2026-04-24 14:58:27
Product description Number Pack Size Price
SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 15 mL D4418 5sets $98
SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 5 mL D4293 5sets $56.4
SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 15 mL D4418 50sets $551
SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 5 mL D4293 50sets $277
3,3′-Diaminobenzidine 99% D12384 1g $45.9
More product size
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3,3'-Diaminobenzidine Properties

Melting point 175-177 °C(lit.)
Boiling point 344.41°C (rough estimate)
Density 1.1726 (rough estimate)
refractive index 1.5000 (estimate)
Flash point 12 °C
storage temp. room temp
solubility 0.55g/l
pka 4.39±0.10(Predicted)
form tablet
color Crystals from MeOH
PH 7 (1g/l, H2O, 20℃)
Water Solubility 0.55 g/L (20 ºC)
Sensitive Light Sensitive
BRN 1212988
Stability Moisture and light sensitive. Incompatible with strong oxidizing agents.
Major Application diagnostic assay manufacturing
hematology
histology
InChI 1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2
InChIKey HSTOKWSFWGCZMH-UHFFFAOYSA-N
SMILES NC1=C(N)C=CC(C2=CC(N)=C(N)C=C2)=C1
CAS DataBase Reference 91-95-2(CAS DataBase Reference)
FDA UNII 2RV4T6KHQI
NIST Chemistry Reference 3,3'-Diaminobenzidine(91-95-2)
EPA Substance Registry System 3,3'-Diaminobenzidine (91-95-2)
UNSPSC Code 12352204
NACRES NA.83

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictogramsGHS hazard pictograms
GHS07,GHS08
Signal word  Danger
Hazard statements  H302-H319-H341-H350
Precautionary statements  P202-P264-P270-P301+P312-P305+P351+P338-P308+P313
PPE dust mask type N95 (US), Eyeshields, Gloves
Hazard Codes  Xn,T,F,O
Risk Statements  36/37/38-40-22-20/21/22-68-45-67-37-34-11-8
Safety Statements  26-36/37-45-36/37/39-22-53-16
RIDADR  UN 3316 9
WGK Germany  1
RTECS  DV8750000
F  8-10-23
TSCA  TSCA listed
HS Code  29214200
Storage Class 6.1C - Combustible acute toxic Cat.3
toxic compounds or compounds which causing chronic effects
Hazard Classifications Acute Tox. 4 Oral
Carc. 1B
Eye Irrit. 2
Muta. 2
Toxicity LDLo orl-rat: 3000 mg/kg CNREA8 26,619,66
NFPA 704
1
2 1

3,3'-Diaminobenzidine price More Price(51)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich D4418 SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 15 mL 91-95-2 5sets $98 2026-04-30 Buy
Sigma-Aldrich D4293 SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 5 mL 91-95-2 5sets $56.4 2026-04-30 Buy
Sigma-Aldrich D4418 SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 15 mL 91-95-2 50sets $551 2026-04-30 Buy
Sigma-Aldrich D4293 SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 5 mL 91-95-2 50sets $277 2026-04-30 Buy
Sigma-Aldrich D12384 3,3′-Diaminobenzidine 99% 91-95-2 1g $45.9 2026-04-30 Buy
Product number Packaging Price Buy
D4418 5sets $98 Buy
D4293 5sets $56.4 Buy
D4418 50sets $551 Buy
D4293 50sets $277 Buy
D12384 1g $45.9 Buy

3,3'-Diaminobenzidine Chemical Properties,Uses,Production

Description

3,3'-Diaminobenzidine (DAB) is a precursor to polybenzimidazole. DAB is frequently used in the immunohistochemical staining of nucleic acids and proteins. It can also be used to detect fingerprints in blood because that it can be oxidized by hydrogen peroxide in the presence of hemoglobin to give a dark-brown color.

Chemical Properties

Off-white to brown solid.

Uses

3,3'-Diaminobenzidine is used as peroxidase substrate and a reagent for spectrophotometric determination of selenium. DAB is used for the immunohistochemical staining of nucleic acids and proteins. As a dark brown dye, was used as an antibody-specific stain to identify the paired antibodies in breast tissue. It may be used for the synthesis of a useful, linear, Schiff-base coordination polymer.

Definition

ChEBI: 3,3'-diaminobenzidine is a member of the class of biphenyls that is benzidine in which one of the hydrogens ortho to each of the amino groups has been replaced by an amino group. It has a role as a histological dye. It is a member of biphenyls and a substituted aniline.

Synthesis

3,3'-Diaminobenzidine can be synthesized by treating 3, 3'-dichlorobenzidine with ammonia in the presence of copper catalyst at high temperature and pressure.

Application

3,3',4,4' -Tetraaminodiphenyl (3,3'-Diaminobenzidine) is suitable for the production of fibers based on polybenzimidazole. These fibers are resistant to high temperature and to chemicals. Fabrics made from them do not burn, do not melt in contact with a flame, and do not become hard when carbonized. They are suitable for the production of protective clothing and flue–gas filters and as substitutes for asbestos.

Preparation

15 kg of 98.5% pure 3,3'-dinitro-4,4'-diaminobiphenyl tide product and 45 kg of methanol,0.075 kg of palladium carbon is mixed, and hydrogen gas of 0.85-0.9 MPa is introduced at 55-60 ° C, and stirred for 3 hours.At the end of the reaction, the catalyst was recovered, methanol was recovered, 30 kg of water was added, and the mixture was centrifuged and dried to obtain 9.3 kg of 3,3'-Diaminobenzidine having a purity of 99.3%.The yield was 90.6% (based on 3,3'-dinitro-4,4'-diacetylaminobiphenyl).

Application

3,3'-Diaminobenzidine is used for preparation of high-strength material such as nucleic acid stain and resistance high-temperature polymers such as bullet-proof vests.

Biochem/physiol Actions

DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in the immunohistological and immunoblotting staining procedures.

Safety Profile

Suspected carcinogen withexperimental tumorigenic data. Moderately toxic byingestion. Mutation data reported. When heated todecomposition it emits toxic fumes of NOx.

References

3,3′-Diaminobenzidine staining interferes with PCR-based DNA analysis
DOI:10.1038/s41598-018-19745-9
Transition metal complexes of novel binuclear Schiff base derived from 3,3′-diaminobenzidine: synthesis, characterization, thermal behavior, DFT, antimicrobial and molecular docking studies
DOI:10.1080/00958972.2020.1752372
3, 3′-Diaminobenzidine with dual o-phenylenediamine groups: two in one enables visual colorimetric detection of nitric oxide
DOI:10.1007/s00216-020-02482-2
A new sensitive colorimetric assay for peroxidase using 3,3'-diaminobenzidine as hydrogen donor.
DOI:10.1016/0003-2697(73)90144-9

3,3'-Diaminobenzidine synthesis

91-94-1
91-95-2
Synthesis of 3,3'-Diaminobenzidine from 3,3'-Dichlorobenzidine

3,3'-Diaminobenzidine Preparation Products And Raw materials

Raw materials

Benzidine

Preparation Products

3,3'-Diaminobenzidine Suppliers

Global( 504)Suppliers
Supplier Tel Email Country ProdList Advantage
Capot Chemical Co.,Ltd. 		+86-（0）57185586718; +8613336195806 sales@capot.com China 29640 60
Jinan Qinmu Fine Chemical Co.,Ltd.
+8618660799346 sales@qinmuchem.com China 1008 58
Chemsigma International Co., Ltd.
+86-2558742998 +86-19951791336 info@chemsigma.com China 1806 58
Fluoropharm Co., Ltd.
+86-0571-85586753 +86-13336034509 sales@fluoropharm.com China 1445 60
Nanjing Triumpharm Co.,Ltd
+86-02586182925 +86-15105172550 info@triumpharm.com China 129 58
Kindchem(Nanjing)Co.,Ltd
+86-025-025-85281586 +8618651653755 sales@kindchem.cn China 1226 58
Beijing CosmicKoda Chemical Technology Co.,Ltd
8615311061293 15311061293 JeanWangCosmicKoda@outlook.com China 49 58
Chemport Science Technology Shanghai) Co., Ltd.
+undefined13816004845 chen@chemport-sh.com China 188 58
Hebei Chuanghai Biotechnology Co., Ltd 		+8615350571055 Sibel@chuanghaibio.com China 8738 58
Wuhan Fortuna Chemical Co., Ltd 		+86-27-59207850 info@fortunachem.com China 5974 58
Supplier Advantage
Capot Chemical Co.,Ltd. 		60
Jinan Qinmu Fine Chemical Co.,Ltd.
58
Chemsigma International Co., Ltd.
58
Fluoropharm Co., Ltd.
60
Nanjing Triumpharm Co.,Ltd
58
Kindchem(Nanjing)Co.,Ltd
58
Beijing CosmicKoda Chemical Technology Co.,Ltd
58
Chemport Science Technology Shanghai) Co., Ltd.
58
Hebei Chuanghai Biotechnology Co., Ltd 		58
Wuhan Fortuna Chemical Co., Ltd 		58

Related articles

Related Qustion

Ask a question
Q:Is 3,3'-Diaminobenzidine toxic?Jac - Mar 9，2026
A:3,3'-Diaminobenzidine (DAB) is a hazardous substance classified as a Group 2B (probable carcinogen) by the International Agency for Research on Cancer (IARC) and is mutagenic. Inhalation can cause skin and eye irritation, allergic reactions, and respiratory problems; long-term exposure may pose health risks. Its toxicity increases upon metabolic activation. When handling, a respirator, chemical goggles, nitrile gloves, and protective clothing must be worn, and the operation must be carried out in a fume hood. The appropriate first aid procedures must be understood.

View Lastest Price from 3,3'-Diaminobenzidine manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
3,3'-Diaminobenzidine pictures 2026-04-30 3,3'-Diaminobenzidine
91-95-2
 US $800.00-730.00 / KG 1KG 98% 100KG/month WUHAN FORTUNA CHEMICAL CO., LTD
3,3'-Diaminobenzidine pictures 2026-04-30 3,3'-Diaminobenzidine
91-95-2
 US $0.00-0.00 / Kg/Drum 1KG 97%min 500KGS WUHAN FORTUNA CHEMICAL CO., LTD
3,3'-Diaminobenzidine pictures 2026-03-20 3,3'-Diaminobenzidine
91-95-2
 US $25.00 / KG 100KG 99.5% 100 mt Hebei Chuanghai Biotechnology Co., Ltd

3,3'-Diaminobenzidine Spectrum

3,3'-Diaminobenzidine(91-95-2)MS3,3'-Diaminobenzidine(91-95-2)1HNMR3,3'-Diaminobenzidine(91-95-2)13CNMR3,3'-Diaminobenzidine(91-95-2)IR13,3'-Diaminobenzidine(91-95-2)IR23,3'-Diaminobenzidine(91-95-2)Raman

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TIMTEC-BB SBB008427 3,3μ,4,4μ-Biphenyltetramine, 3,3μ,4,4μ-Tetraaminobiphenyl, DAB 1,1'-Biphenyl-3,3',4,4'-tetrakisamine 3,3',4,4'-[1,1'-Biphenyl]tetraamine 3,3',4,4'-Biphenyltetraamine 3,3'-Diaminobenzidine,99% 3,3′-Diaminobenzidine,3,3′,4,4′-Biphenyltetramine, 3,3′,4,4′-Tetraaminobiphenyl, DAB 3.3'-Diaminobenzidine 250mg [91-95-2] 3,3'-Diaminobenzidin 3'-DiaMinobenzidine TETRAAMINOBIPHENYL Biphenyl-3,3′,4,4′-tetrayltetraamin 3,3',4,4'-Biphenyltetramine, DAB 3,3'-Diaminobenzidine [for Biochemical Research] Biphenyl-3,3 , 4, 4 -tetrayltetraamin (Salts of) 3,3',4,4'-Tetraaminobiphenyl DAB [1,1'-BIPHENYL]-3,3',4,4'-TETRAMINE 3,3',4,4'-TETRAAMINOBIPHENYL 3,3',4,4'-TETRAAMINODIPHENYL 3,3',4,4'-BIPHENYLTETRAMIN 3,3',4,4'-BIPHENYLTETRAMINE 3,4,3',4'-TETRAAMINOBIPHENYL biphenyl-3,3',4,4'-tetraamine BIPHENYL-3,3',4,4'-TETRAMINE 3,3',4,4'-Diphenyltetramine 3,3',4,4'-Tetraminobiphenyl 3,3’,4,4’-diphenyltetramine 3,3’-diaminobenzidene 3,3' biphenyl-3,3,4,4-tetrayltetraamine DAB (3.3'-DiaMinobenzidine) Biotechnology Grade 3,3'-Diaminobenzidine purum, >=98.0% (CHN) SIGMAFAST? 3,3′-Diaminobenzidine tablets 3,3'-Diaminobenzidine Base 4-(3,4-Diaminophenyl)benzene-1,2-diamine JACS-91-95-2 3,3'-Diaminobenzidine > 3,3'-Diaminobenzidine@100 μg/mL in Toluene 3,3'-Diaminobenzidine[forBiochemicalResearch]> High purity 3,3'-Diaminobenzidine 3,3'-DIAMINOBENZIDINE 91-95-2 3,3'-Diaminobenzidine ISO 9001：2015 REACH 3,3-Diaminobenzidine, GR 99% top 3,3'-Diaminobenzidine 3,3-Diaminobenzidine (DAB) pure, 98% 3,3'-Diaminobenzidine IN STOCK 3,3'-Diaminobenzidine Factory 3,3'-Diaminobenzidine COA TDS MSDS 3，3-Diamonobenzidine [1,1'-biphenyl]-3,3',4,4'-tetraaMine Diaminobenzidine,DAB DAB (3,3'-Diaminobenzidine) 3,3'-Diaminobenzidine CAS 3,3'-Diaminobenzidine 1,1'-Biphenyl]-3,3',4,4'-tetraamine 3,3'-Diaminobenzidine(DAB) 3,3'-Diaminobenzidine in acetonitrile 3,3''-Diaminobenzidine