DMT-dC(bz) Phosphoramidite

DMT-dC(bz) Phosphoramidite 속성

DMT-dC(bz) Phosphoramidite C화학적 특성, 용도, 생산

화학적 성질

DMT-dC(bz) Phosphoramidite, also known as 5'-O-DMT-N4-Benzoyl-2'-Deoxycytidine-CE Phosphoramidite or (2R,3R,4R,5R)-2-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-5-(2-isobutyramido-6-oxo-1,6-dihydro-9H-purin-9-yl)-4-(2-methoxyethoxy)tetrahydrofuran-3-yl (2-cyanoethyl) diisopropylphosphoramidite (IUPAC Name), is a novel nucleoside amidite analog, which can be subjected in the synthesis of DNA.
DMT-dC(bz) Phosphoramidite belongs to the group of DNA Phosphoramidites.Its key features include:
Exocyclic amine functions are protected by a benzoyl group (dA(bz) anddC(bz)) or isobutyryl group (dG(ib))
Recommended cleavage and deprotection conditions are 8 hours at 55 °Cor 24 hours at room temperature using concentrated ammonia solution, for standard base-protected oligonucleotides
The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides.

용도

5'-O-DMT-N4-Benzoyl-2'-deoxycytidine 3'-CE phosphoramidite is used to prepare antisense oligonucleotides containing conformationally constrained methoxyaminomethylene and aminooxymethylene and aminomethylene bridged nucleoside analogs.

Synthesis

These Examples illustrate the phosphitylation of several protected nucleoside reagents with 2-Cyanoethyl-N,N,N',N'-tetraisopropylphosphordiamidite in the presence of several activators according to the present invention. Eleven phosphitylation reactions (1-11) comprising reacting a protected nucleoside reagent with 2-Cyanoethyl-N,N,N',N'-tetraisopropylphosphordiamidite in the presence of an acid-base activator according to the present invention were conducted, and the product yields of each calculated, as described in the General Procedure, below. The various combinations of protected nucleoside, activator base, activator acid, solvent, and yield for each of the 11 reactions are listed in Table 1. General Procedure: The activator base (1.1 to 1.2 equivalents) is added to the solvent and 0.95 to 1.1 equivalents of activator acid is subsequently added thereto at ambient temperature to form the activator solution. About 1 equivalent of the protected nucleoside is dissolved in about 10 equivalents of the solvent in a separate vessel and about 3 equivalents of the solvent is then distilled off under reduced pressure. About 1 to 1.2 equivalents of 2-Cyanoethyl-N,N,N',N'-tetraisopropylphosphordiamidite is added to the nucleoside mixture at ambient temperature, and the activator solution prepared previously is then added to the nucleoside mixture at ambient temperature with vigorous stirring. After 12 hours, the reaction mixture is diluted with toluene and washed with water. The organic layer is separated, dried over sodium sulfate if necessary, and concentrated under reduced pressure. The yield of the desired amidite is then calculated using HPLC techniques, that is, the resulting product mixture is run through an HPLC column using an appropriate eluent, and the area under the HPLC peaks used to determine the %yield of product in the mixture.

DMT-dC(bz) Phosphoramidite 준비 용품 및 원자재

원자재

준비 용품

DMT-dC(bz) Phosphoramidite 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Zhejiang Hengkang Pharmaceutical Co., Ltd.	+86-576-83372028 +86-18868723926	bd1@hengkangpharm.cn	China	119	58
Chemtour Biotech Co., Ltd	+8617327281506	market@chemtour.com	China	1472	58
Hefei Huana Biomedical Technology Co.,Ltd.	+86-15900695956	shiqin.he@huanaok.com.cn	China	96	58
career henan chemical co	+86-0371-86658258	sales@coreychem.com	China	29914	58
Jinan Carbotang Biotech Co.,Ltd.	+8615866703830	figo.gao@foxmail.com	China	7019	58
Hubei Jusheng Technology Co.,Ltd.	18871490254	linda@hubeijusheng.com	CHINA	28180	58
Yunbio Tech Co.,Ltd.	+86-010-60605551 +86-18046518538	yunbiochem@126.com	China	321	58
Nanjing Baifuli Technology Co., Ltd.	+86-15335185688	sales@unisyn.cn	CHINA	332	58
Chongqing Chemdad Co., Ltd	+86-023-61398051 +8613650506873	sales@chemdad.com	China	39916	58
Alchem Pharmtech,Inc.	8485655694	sales@alchempharmtech.com	United States	63711	58

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