D-苏式-鞘胺醇-1-磷酸

S1P (1 μM) induces a significant Ca ²⁺ releases in HEK293 cells under serum starvation conditions (1% FCS).In a functional Ca ²⁺ assay, Suramin (HY-B0879) alone does not exert any effect on intracellular Ca ²⁺ release via gpr3, gpr6 or gpr12. In contrast, S1P (1 μM) induces Ca ²⁺ release of gpr3, gpr6 and gpr12 in the presence of Suramin (HY-B0879) various concentrations in transfected HEK293 cells. In a functional Ca ²⁺ assay,S1P (3-3000 nM) in the presence Suramin (300 μM), exhibits nanomolar EC ₅₀ values for gpr3 (EC ₅₀ =29 nM), gpr6 (EC ₅₀ =15 nM) and gpr12 (EC ₅₀ =24 nM), rat gpr3 (EC ₅₀ =68 nM),respectively in HEK293 cells. S1P increases intracellular calcium levels in TAg-Jurkat cells expressing S1P1 and G _qi5 , which allows for phospholipase C stimulation by G _i proteins, when used at a concentration of 200 nM, as well as in TAg-Jurkat cells expressing S1P2 and S1P3 receptors (EC ₅₀ s = 8 and 11 nM, respectively).