NVP-231
| 中文名称 | NVP-231 |
|---|---|
| 中文同义词 | CERK抑制剂(NVP 231);化合物NVP 231;N-(2-苯甲酰胺基苯并噻唑-6-基)金刚烷-1-甲酰胺 |
| 英文名称 | N-[2-(Benzoylamino)-6-benzothiazolyl]tricyclo[3.3.1.13,7]decane-1-carboxamide |
| 英文同义词 | adamantane-1-carboxylic acid (2-benzoylamino-benzothiazol-6-yl)amide;N-[2-(Benzoylamino)-6-benzothiazolyl]tricyclo[3.3.1.13,7]decane-1-carboxamide;NVP 231;NVP-231;NVP231;CS-1722;NVP-231;NVP 231;NVP231;Tricyclo[3.3.1.13,7]decane-1-carboxamide, N-[2-(benzoylamino)-6-benzothiazolyl]-;NVP231,inhibit,mcf-7,cancer,cerk,Apoptosis,NVP 231,Inhibitor,NCI-H358,NVP-231 |
| CAS号 | 362003-83-6 |
| 分子式 | C25H25N3O2S |
| 分子量 | 431.55 |
| EINECS号 | |
| 相关类别 | 小分子抑制剂;抑制剂;小分子抑制剂，天然产物;细胞生物学试剂;Inhibitors |
| Mol文件 | 362003-83-6.mol |
| 结构式 |  |
NVP-231 性质
| 熔点 | 246-248 °C(Solv: isopropanol (67-63-0); water (7732-18-5)) |
|---|---|
| 密度 | 1.398±0.06 g/cm3(Predicted) |
| 储存条件 | Store at +4°C |
| 溶解度 | 二甲基亚砜：>10mg/mL |
| 酸度系数(pKa) | 9.09±0.70(Predicted) |
| 形态 | 粉末 |
| 颜色 | 白色至类白色 |
| 稳定性 | 可在-20°C下的DMSO溶液中保存长达1个月。 |
| InChIKey | MVSSJPGNLQPWSW-UHFFFAOYSA-N |
| SMILES | C12(C(NC3C=C4SC(NC(=O)C5=CC=CC=C5)=NC4=CC=3)=O)CC3CC(CC(C3)C1)C2 |
| Target | Value |
|
CreK
(Cell-free assay) | 12 nM |
NVP-231 (0-500 nM; 24 hours) gradually reduces the cellular CerK activity, as measured by NBD-C1P formation, demonstrating that NVP-231 active in transfected cells. The IC 50 for CerK in this cellular system is 59.70 ± 12 nM. NVP-231 (0-1000 nM; 48 hours) decreases cell viability as a dose-dependent manner. This compound shows IC 50 values of 1 μM in MCF-7 cells and 500 nM in NCI-H358 cells. NVP-231 (1 μM; 24-72 hours) induces caspase-3 and caspase-9 cleavage in both cell lines. However, the highest caspase-3 and caspase-9 cleavage and activation occurred at 24 hours in MCF-7 cells, then decreases again. In NCI-H358 cells, caspase-3 and caspase-9 cleavage occurrs continuously over 72 hours. NVP-231 (0-500 nM; 24 hours) causes a concentration-dependent up-regulation of cyclin B1 phosphorylation at Ser133 and a reduction of CDK1 phosphorylation at Tyr15. The total CDK1 expression also declined upon CerK inhibition.
Cell Viability Assay
| Cell Line: | MCF-7 cells; NCI-H358 cells |
| Concentration: | 0 nM, 10 nM, 100 nM, 300 nM, 500 nM, 1000 nM |
| Incubation Time: | 48 hours |
| Result: | Reduced MCF-7 cells and NCI-H358 cells in a concentration manner. |
Apoptosis Analysis
| Cell Line: | MCF-7 cells; NCI-H358 cells |
| Concentration: | 1000 nM |
| Incubation Time: | 24-72 hours |
| Result: | Increases caspase-3 and caspase-9 cleavage in MCF-7 and NCI-H358 cells. |
Western Blot Analysis
| Cell Line: | MCF-7 cells; NCI-H358 cells |
| Concentration: | 0 nM, 100 nM, 300 nM, 500 nM |
| Incubation Time: | 24 hours |
| Result: | Decreased p-cyclin B1, p-CDK1 as a concentration manner. |
安全信息
| WGK Germany | nwg |
|---|