赫斯特荧光染料 33258
| 中文名称 | 赫斯特荧光染料 33258 |
|---|---|
| 中文同义词 | 赫斯特荧光燃料，33258;甲嗪双苯咪酚;双苯咪唑;HOECHST 33258 溶液;赫斯特荧光染料 33258 溶液;2'-(4-羟苯基)-5-(4-甲基-1-哌嗪基)-2,5'-联苯并咪唑三盐酸盐水合物 [用于生化];BIS BENZIMIDE HOECHST NO33258 荧光染料;赫斯特33258 |
| 英文名称 | Hoechst 33258 |
| 英文同义词 | Bis-BenzimideFluorochrome;p-(5-(5-(4-Methyl-1-piperazinyl)-1H-2-benzimidazolyl)-1H-2-benzimidazolyl)phenol trihydrochloride;Hoechst(R) 33258,98%;bisBenzimide, 2μ-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi-1H-benzimidazole hydrate trihydrochloride;HOE 33258, Hoechst 33258, 2μ-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride;4-[5-[6-(4-Methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1,3-dihydrobenzimidazol-2-ylidene]cyclohexa-2,5-dien-1-one trihydrochloride;Phenol, p-(5-(5-(4-methyl-1-piperazinyl)-2-benzimidazolyl)-2-benzimidazolyl)-, trihydrochloride;Pibenzimol HCl |
| CAS号 | 23491-45-4 |
| 分子式 | C25H25ClN6O |
| 分子量 | 460.97 |
| EINECS号 | 245-690-6 |
| 相关类别 | 荧光探针，标记，颗粒和染色;生化试剂;其他生化试剂;细胞信号和神经生物学;细胞生物学;色素类;生化试剂-色素类;试剂;细胞分析;细胞死亡;细胞相关;化学试剂;细胞生物学试剂;分析试剂-显色剂;生物化工;通用生化试剂-生物染色剂与化学指示剂;有机原料 |
| Mol文件 | 23491-45-4.mol |
| 结构式 |  |
赫斯特荧光染料 33258 性质
| 熔点 | 314 °C |
|---|---|
| 密度 | 1.5099 (rough estimate) |
| 折射率 | 1.6300 (estimate) |
| 储存条件 | -20°C |
| 溶解度 | 在水中的溶解度10 mg/mL |
| 形态 | 粉末 |
| 颜色 | 黄色至深黄色 |
| 水溶解性 | SOLUBLE |
| 激发峰(Ex)/发射峰(Em) | λex 345 nm; λem 507 nm in H2O |
| BRN | 4088183 |
| 稳定性 | 吸湿性 |
| InChIKey | UDJDXLXTHZQAAC-UHFFFAOYSA-N |
| SMILES | CN1CCN(C2C=CC3NC(C4C=CC5NC(C6C=CC(O)=CC=6)=NC=5C=4)=NC=3C=2)CC1.Cl |
| CAS 数据库 | 23491-45-4(CAS DataBase Reference) |
| EPA化学物质信息 | Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]-, trihydrochloride (23491-45-4) |
IC50: 51.31±4.56 μM (HeLa cell), 32.43±3.27 μM (HL60 cell), 15.42±2.16 μM (U937 cell)
Hoechst 33258, a fluorescent compound with a head-to-tail bis-benzimidazole structure, is initially found to be cytotoxic against L1210 murine leukemia. Hoechst 33258 is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), Human promyelocytic leukemia cell (HL60) and U937 cell Line. The IC 50 determined in the case of HeLa, HL60 and U937 is 51.31±4.56, 32.43±3.27 and 15.42±2.16 μM for Hoechst 33258, respectively. The cytotoxic property of Hoechst 33258 is investigated on a panel of seven tumour cell lines of different histological origin and Madine-Darby canine kidney (MDCK) normal cells. All cell lines, except MCF-7, exposed to Hoechst 33258 exhibit GI 50 from 84×10 -6 to 191.5×10 -6 mol/dm 3 . Under the same experimental conditions, Hoechst 33258, used as a binder reference compound, stops the cell cycle in S phase and G0/G1.
安全信息
| 危险品标志 | Xn |
|---|---|
| 危险类别码 | 22-36/38-36/37/38-20/21/22 |
| 安全说明 | 26-36-24/25-23 |
| WGK Germany | 3 |
| RTECS号 | SM1140500 |
| F | 3-8-10 |
| TSCA | TSCA listed |
| 海关编码 | 32129000 |
| 存储类别 | 11 - 可燃固体 |
| 毒性 | dog,LD,intravenous,> 22400ug/kg (22.4mg/kg),LUNGS, THORAX, OR RESPIRATION: DYSPNEASENSE ORGANS AND SPECIAL SENSES: LACRIMATION: EYEGASTROINTESTINAL: CHANGES IN STRUCTURE OR FUNCTION OF SALIVARY GLANDS,National Technical Information Service. Vol. PB84-171206, |
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2026/09/15 | HY-15558A | 赫斯特荧光染料 33258 Hoechst 33258 trihydrochloride | 23491-45-4 | 25 mg | 435元 |
| 2026/09/15 | HY-15558A | 赫斯特荧光染料 33258 Hoechst 33258 trihydrochloride | 23491-45-4 | 10 mM * 1 mLin DMSO | 478元 |