- Tetrazolium Red
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- $29.00 / 1mL
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2026-04-23
- CAS:298-96-4
- Min. Order:
- Purity: 99.82%
- Supply Ability: 10g
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| | 2,3,5-Triphenyltetrazolium chloride Basic information |
| | 2,3,5-Triphenyltetrazolium chloride Chemical Properties |
| Melting point | 250 °C(lit.) | | Boiling point | 490.96°C (rough estimate) | | density | 1.1704 (rough estimate) | | bulk density | 230kg/m3 | | refractive index | 1.6110 (estimate) | | storage temp. | 2-8°C | | solubility | H2O: may be clear to slightly hazy | | form | powder | | color | faint yellow | | PH | 3.7 (20°C, 10g/L in H2O) | | Water Solubility | Soluble in water, ethanol and acetone. Insoluble in ether. | | λmax | 247 nm | | Sensitive | Light Sensitive & Hygroscopic | | Merck | 14,9744 | | BRN | 3923356 | | Stability: | Stable. Light sensitive. Protect from moisture. Incompatible with strong oxidizing agents. | | Major Application | diagnostic assay manufacturing
hematology
histology | | Biological Applications | Algae viability assay; microbial growth assays; transketolase activity screening assays; detecting bacteria,g-hydroxybutyric acid (GHB),microorganisms,myocardial infarction; measuring dehydrogenase activity; treating cancer | | InChI | 1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1 | | InChIKey | PKDBCJSWQUOKDO-UHFFFAOYSA-M | | SMILES | [Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4 | | CAS DataBase Reference | 298-96-4(CAS DataBase Reference) | | EPA Substance Registry System | 2,3,5-Triphenyltetrazolium chloride (298-96-4) |
| Hazard Codes | Xi,Xn | | Risk Statements | 36/37/38-20/21/22 | | Safety Statements | 26-36 | | WGK Germany | 3 | | RTECS | XF8100000 | | F | 3-8-10 | | TSCA | TSCA listed | | HS Code | 29339900 | | Storage Class | 4.1B - Flammable solid hazardous materials | | Hazard Classifications | Eye Irrit. 2
Flam. Sol. 1
Skin Irrit. 2 | | Toxicity | LD50 intravenous in mouse: 5600ug/kg |
| | 2,3,5-Triphenyltetrazolium chloride Usage And Synthesis |
| Chemical Properties | white powder | | Uses | 2,3,5-Triphenyl-2H-tetrazolium chloride is used as a dye in cell biological studies. | | Uses | 2,3,5-Triphenyltetrazolium chloride is a selective serotonin 5-HT4 receptor agonist and 5-HT3 gastrointestinal prokinetic agent for functional dyspepsia | | Uses | In analytical chemistry as a sensitive reagent for reducing sugars, and to distinguish between a-ketols and simple aldehydes. For staining plant and animal tissue. Germination indicator in testing the ability of seeds to germinate: G. Lakon, Ber. Dtsch. Bot. Ges. 60, 299, 434 (1942). In histochemical studies. In determination of antibiotics; of dehydrogenases. | | Definition | ChEBI: An organic chloride salt having 2,3,5-triphenyltetrazolium as the counterion. | | General Description | 2,3,5-Triphenyltetrazolium chloride is a colorless water-soluble dye. In the mitochondria of living cells, it is reduced to a deep red, water-insoluble compound (formazan). 2,3,5-Triphenyltetrazolium chloride helps to distinguish between viable and infarcted brain tissue after stroke. | | Synthesis | E. The 2,3,5-triphenylformamidine (IV-1) solid obtained in Step D was mixed with 300 mL of dichloromethane and 300 mL of water. After the solid was completely dissolved, N-chlorosuccinimide (0.11 mol, 14.7 g) was added and the reaction was carried out with stirring at 0 °C for 5 h. F. The reaction mixture from Step E was allowed to partition and the upper aqueous phase was separated. The organic phase was distilled under reduced pressure to remove the solvent to give the crude product 2,3,5-triphenyltetrazole (V-1). The crude product was purified by recrystallization with chloroform to give 30.2 g of pure product (yield: 90%). | | Purification Methods | Crystallise TTZ from EtOH or CHCl3, and dry it at 105o. [Beilstein 26 H 363, 26 II 216, 26 III/IV 1774.] | | References | [1] Indian Journal of Chemistry, Section B: Organic Chemistry Including Medicinal Chemistry, 1983, vol. 22, # 8, p. 771 - 775
[2] J. Appl. Chem. USSR (Engl. Transl.), 1983, vol. 56, # 3, p. 617 - 621
[3] Zhurnal Prikladnoi Khimii (Sankt-Peterburg, Russian Federation), 1983, vol. 56, # 3, p. 649 - 653
[4] Patent: CN108440433, 2018, A. Location in patent: Paragraph 0057; 0058; 0059; 0064; 0065
[5] Indian Journal of Chemistry, Section B: Organic Chemistry Including Medicinal Chemistry, 1981, vol. 20, # 5, p. 404 - 406 |
| | 2,3,5-Triphenyltetrazolium chloride Preparation Products And Raw materials |
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