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Common reductant Uses
Chemical Name:
DTT;DITT;D-Dtt;DL-DTT;DTT-MBG;wr34678;CGMP DTT;DTT, 10G;DTT, 25G;Sputolysin
Molecular Formula:
Formula Weight:
MOL File:

DL-1,4-Dithiothreitol Properties

Melting point:
41-44 °C(lit.)
Boiling point:
125 °C
1.04 g/mL at 20 °C
Flash point:
>230 °F
storage temp. 
H2O: 50 mg/mL, clear, colorless
4.0-6.0 (20-25℃, 0.1m in H2O)
Water Solubility 
freely soluble
Air Sensitive
Stability Stable, but heat sensitive. Incompatible with strong oxidizing agents. Keep frozen at -20 to -10 C.
CAS DataBase Reference
3483-12-3(CAS DataBase Reference)
NIST Chemistry Reference
2,3-Butanediol, 1,4-dimercapto-, (r*,r*)-(3483-12-3)
EPA Substance Registry System
2,3-Butanediol, 1,4-dimercapto-, (2R,3R)-rel-(3483-12-3)
  • Risk and Safety Statements
  • Hazard and Precautionary Statements (GHS)
Hazard Codes  Xn
Risk Statements  25-36/37/38-22-20/21/22
Safety Statements  26-45-37/39-36
WGK Germany  3
RTECS  EK1610000
HS Code  29309099
Signal word: Warning
Hazard statements:
Code Hazard statements Hazard class Category Signal word Pictogram P-Codes
H302 Harmful if swallowed Acute toxicity,oral Category 4 Warning P264, P270, P301+P312, P330, P501
H315 Causes skin irritation Skin corrosion/irritation Category 2 Warning P264, P280, P302+P352, P321,P332+P313, P362
H319 Causes serious eye irritation Serious eye damage/eye irritation Category 2A Warning P264, P280, P305+P351+P338,P337+P313P
H335 May cause respiratory irritation Specific target organ toxicity, single exposure;Respiratory tract irritation Category 3 Warning
H412 Harmful to aquatic life with long lasting effects Hazardous to the aquatic environment, long-term hazard Category 3 P273, P501
Precautionary statements:
P261 Avoid breathing dust/fume/gas/mist/vapours/spray.
P264 Wash hands thoroughly after handling.
P264 Wash skin thouroughly after handling.
P270 Do not eat, drink or smoke when using this product.
P273 Avoid release to the environment.
P280 Wear protective gloves/protective clothing/eye protection/face protection.
P304+P340 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P337+P313 IF eye irritation persists: Get medical advice/attention.
P405 Store locked up.
P501 Dispose of contents/container to..…

DL-1,4-Dithiothreitol price More Price(54)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 233153-M Cleland’s Reagent, ULTROL 3483-12-3 1gm-m $84.5 2017-11-08 Buy
Sigma-Aldrich 233153-M Cleland’s Reagent, ULTROL 3483-12-3 5gm-m $267 2017-11-08 Buy
TCI Chemical D1071 DL-Dithiothreitol >98.0%(T) 3483-12-3 1g $34 2017-11-08 Buy
TCI Chemical D1071 DL-Dithiothreitol >98.0%(T) 3483-12-3 5g $88 2017-11-08 Buy
Alfa Aesar A15797 1,4-Dithio-DL-threitol, 98% 3483-12-3 5g $139 2017-11-08 Buy

DL-1,4-Dithiothreitol Chemical Properties,Uses,Production

Common reductant

DL-1, 4-DL-1, 4-Dithiothreitol is a common reducing agent, also known as DTT with a strong reduction capability. Its reduction capability is largely due to the conformational stability of the six-membered ring (containing disulfide bone) of its oxidation state. The reducing capability is affected by the pH value and also takes effect only in the case of a pH being greater than 7. This is because it is only the deprotonated thiolate anion (-S-) that is actively in reduction reaction while thiol (an-SH) is not; and the pKa of mercapto group is typically around~8.3.
The reduction of the disulfide bond mediated by DL-1, 4-Dithiothreitol mainly consists of a two-step continuous sulfhydryl-disulfide exchange: wherein the intermediate state formed by the first step of the reaction is very unstable because the secondary sulfhydryl group of DTT tends to connected by the oxidized sulfur atom, making the intermediate state be rapidly converted to the cyclic oxidation structure of DTT, thus completing the reduction of disulfide bonds.
Since being easily oxidized by air, the stability of DL-1, 4-Dithiothreitol (DTT) is relatively poor; frozen storage or processing in inert gas can extend its duration life. Owing to the low nucleophilicity of the protonated sulfur, with the decreased pH value, the effective reducing capability of DTT is also reduced; Moreover, Tris (2-carboxyethyl) phosphine HCl (TCEP hydrochloride) can be used as the substitute of DTT at low pH value and is also more stable than DTT.
DL-1, 4-Dithiothreitol has an antioxidant property that can protect the reductive groups on the enzyme molecules and maintain a reducing environment and stabilize the enzyme activity. DTT also contain certain RNase inhibitors which can inhibit the enzymatic activity of RNase. It has a similar effect as mercaptoethanol (b-mercaptoethanol) but the irritating odor and toxicity of DTT is much smaller and lower than the toxicity of mercaptoethanol. Moreover, DTT has a similar effect as mercaptoethanol even at a concentration of being seven times lower, but the price of DTT is slightly higher. It can be used as the protein thiol group protecting agent and the cleavage of the protein disulfide bond as well as sequence analysis.
Current implemented law regarding the poisons and toxic materials has designated β-mercaptoethanol and reagent-containing β-mercaptoethanol as poison. DL-1, 4-Dithiothreitol has similar effect as β-mercaptoethanol and can protect the SH group of protein, cutting the disulfide bond and therefore will be gradually paid attention by related applications.
The above information is edited by the chemicalbook of Dai Xiongfeng.


Molecular Biology

Chemical Properties

White crystalline powder


Dithiothreitol is a redox reagent commonly used as a reducing agent for thiolated DNA. Dithiothreitol is also used to reduce the disulfide bonds of proteins.


ChEBI: The threo-diastereomer of 1,4-dimercaptobutane-2,3-diol.

Biological Activity

Reagent for maintaining SH groups in the reduced state.

DL-1,4-Dithiothreitol Preparation Products And Raw materials

Raw materials

Preparation Products

DL-1,4-Dithiothreitol Suppliers

Global( 293)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Henan Tianfu Chemical Co.,Ltd.
0371-55170693 CHINA 20809 55
Mainchem Co., Ltd.
+86-0592-6210733 CHINA 32766 55
Shanghai Zheyan Biotech Co., Ltd.
18017610038 CHINA 300 58
Suzhou Highfine Biotech Co., Ltd +86-512-68071238 69209928 86880809 68311833
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Hunan Hui Bai Shi Biotechnology Co., Ltd. 0731-85526065 13319523173 13308453923
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010-62340519 China 1978 55
Dalian Meilun Biotech Co., Ltd. 0411-66771943;0411-66771942
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Shanghai Aladdin Bio-Chem Technology Co.,LTD 021-20337333/400-620-6333
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Nanjing PengRui Biological Co., Ltd. 0731-88551033 18175154369
0731-88551033 China 842 58
Shanghai Qiao Chemical Science Co., Ltd 021-50396381 021-58892003 China 3508 65

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