Company Name: |
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Tel: |
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Email: |
sales@sdfine.com |
Products Intro: |
Cas:298-83-9
ProductName:p-Nitro-Blue tetrazolium chloride
Purity: 99% | Package: 1 kg,5 kg, 10 kg,25kg and 1 MT
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Company Name: |
Shanghai Yanze Chemical Co., Ltd
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Tel: |
021-13621551597 19975051755 |
Email: |
3577422208@qq.com |
Products Intro: |
Cas:298-83-9
ProductName:Nitro Blue Tetrazolium Chloride monohydrate
CustNote: 10T
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Company Name: |
Shanghai Haohong Pharmaceutical Co., Ltd.
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Tel: |
400-8210725 4008210725 |
Email: |
malulu@leyan.com |
Products Intro: |
Cas:298-83-9
ProductName:Nitro blue tetrazolium chloride
Purity: 97% | Package: 100mg
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- Tetrazolium Blue Chloride
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- $15.00 / 1KG
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2021-07-13
- CAS:1871-22-3
- Min. Order: 1KG
- Purity: 99%+ HPLC
- Supply Ability: Monthly supply of 1 ton
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| Nitro Blue Tetrazolium Chloride Basic information |
Product Name: | Nitro Blue Tetrazolium Chloride | Synonyms: | 2h-tetrazolium,3,3’-(3,3’-dimethoxy[1,1’-biphenyl]-4,4’-diyl)bis[2-(4-nitrophe;nbt(dye);nitrobluetetrazoliumsalt;nitrotetrazoliumbt;nitrotetrazoliumchlorideblue;tetrazoliumnitroblue;tetrazoliumnitrobt;2H-TETRAZOLIUM,2,2'-((3,3')-DIMETHOXY[1,1'-BIPHENYL]-4,4'-DIYL)BIS[3-(4-NITROPHENYL)-5-PHENYL-],DICHLORIDE | CAS: | 298-83-9 | MF: | C40H30ClN10O6+ | MW: | 782.19 | EINECS: | 206-067-4 | Product Categories: | Tetrazolium salt;Organics;Tetrazolium Salts;Tetrazolium Salts & Formazans;substrate;298-83-9 | Mol File: | 298-83-9.mol | |
| Nitro Blue Tetrazolium Chloride Chemical Properties |
Melting point | 200°C | density | 1.5521 (rough estimate) | refractive index | 1.7350 (estimate) | storage temp. | 2-8°C | solubility | methanol: 50 mg/mL, clear, very strongly yellow | form | Powder/Solid | color | Yellow | Water Solubility | Soluble in water, ethanol and methanol. | Sensitive | Light Sensitive | λmax | 256 nm | BRN | 4115923 | Stability: | Stable, but store cold. | Biological Applications | Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting | EPA Substance Registry System | 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride (298-83-9) |
| Nitro Blue Tetrazolium Chloride Usage And Synthesis |
Description | Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate. | Chemical Properties | NBT is a yellow crystals that can soluble in water, aqueous buffers, and dimethylformamide. Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology. | Uses | Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate. | Application | Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells. It has been used as a substrate for western blot detection. It has been used for the staining of histological tissue sections. Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease. It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Further, it is used in immunology for detection of alkaline phosphatase. | Definition | ChEBI: Nitro blue tetrazolium dichloride is an organic chloride salt having nitro blue tetrazolium(2+) as the counterion. It contains a nitro blue tetrazolium(2+). | General Description | Nitrotetrazolium Blue chloride (NBT) is a colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease. | Hazard | Moderately toxic by ingestion. | Biochem/physiol Actions | Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target. | Safety Profile | Moderately toxic by ingestion. When heated to decomposition it emits toxic vapors of NOx and Clí. | storage | +4°C |
| Nitro Blue Tetrazolium Chloride Preparation Products And Raw materials |
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