| Identification | Back Directory | [Name]
Methyl Green zinc chloride salt | [CAS]
7114-03-6 | [Synonyms]
CI 42590
CI NO 42590
LIGHT GREEN B
DOUBLE GREEN SF
METHYL GREEN, CERTIFIED
Methyl green (CI x 42590)
METHYL GREEN, ZINC CHLORIDE
METHYL GREEN, FOR MICROSCOPY
ETHYL GREEN ZINC CHLORIDE SALT
METHYL GREEN, ZINC CHLORIDE SALT
METHYL GREEN CRYSTAL VIOLET-FREE
FERRIC NITRATE NONAHYDRATE ACS GRADE
METHYL GREEN, ZINC CHLORIDE SALT, CERTIF IED
Methyl Green, zinc chloride salt, for analysis
Methyl Green, zinc chloride salt,pure,certified
Methyl Green, zinc chloride salt, certified, pure
Methyl Green, zinc chloride salt,pure,high purity biological stain
Methyl Green, zinc chloride salt, high purity biological stain, pure
Methyl green, crystal violet free, (4-((4-(Dimethylamino)phenyl)(4-(dimethyliminio)cyclohexa-2,5-dien-1-ylidene)methyl)-N-ethyl-N,N-dimethylanilinium bromide chloride zinc chloride salt
Benzenaminium, 4-4-(dimethylamino)phenyl4-(dimethyliminio)-2,5-cyclohexadien-1-ylidenemethyl-N-ethyl-N,N-dimethyl-, bromide chloride, compd. with zinc chloride (ZnCl2) | [EINECS(EC#)]
230-415-4 | [Molecular Formula]
C27H35BrCl3N3Zn | [MDL Number]
MFCD00151094 | [MOL File]
7114-03-6.mol | [Molecular Weight]
653.24 |
| Chemical Properties | Back Directory | [Appearance]
maroon to reddish-brown crystals | [Melting point ]
>300 °C(lit.)
| [storage temp. ]
Store at RT. | [solubility ]
Soluble in water, ethanol; insoluble in xylene | [form ]
Solid | [color ]
Green | [PH Range]
Yellow (0.1) to greenish-blue (2.3) | [Stability:]
Stable. Incompatible with strong oxidizing agents. | [Water Solubility ]
70 g/L (20 ºC) | [λmax]
629 nm, 423 nm | [Biological Applications]
Detecting phosphates,polyphosphates,tocopherol,tocopherol acetate |
| Hazard Information | Back Directory | [Chemical Properties]
maroon to reddish-brown crystals | [Uses]
Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently. | [Purification Methods]
Crystallise the dye from hot water. [Beilstein 13 IV 2286.] |
| Questions And Answer | Back Directory | [Description]
Methyl Green, Zinc chloride salt (chemical formula: C27H35Cl4N3Zn) is the zinc chloride salt form of Methyl green. Methyl green is a cationic or positive charged stain. Since it can binds selectively to the AT-rich regions of native DNA, it is mainly used for the DNA and RNA staining in the cell nuclei. Methyl Green, Zinc chloride Salt, as a CERTISTAIN® dry dye for DNA staining, it has many application: used as a tracking dye in acid buffers for electrophoresis; used to demonstrate DNA and RNA in tissues sections in the Methyl Green-Pyronin method; used as an exclusion viability stain for cells. In addition, it can also be used in fluorimetric assay and flow cytometry.
| [References]
http://www.sigmaaldrich.com/catalog/product/sigma/67060?lang=zh®ion=CN
https://pubchem.ncbi.nlm.nih.gov/compound/3084002#section=Canonical-SMILES
https://en.wikipedia.org/wiki/Methyl_green
http://www.abcam.com/methyl-green-zinc-chloride-salt-ab146287.html
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