| Identification | Back Directory | [Name]
Methyl Green zinc chloride salt | [CAS]
7114-03-6 | [Synonyms]
CI 42590
CI NO 42590
LIGHT GREEN B
DOUBLE GREEN SF
METHYL GREEN, CERTIFIED
Methyl green (CI x 42590)
METHYL GREEN, ZINC CHLORIDE
METHYL GREEN, FOR MICROSCOPY
ETHYL GREEN ZINC CHLORIDE SALT
METHYL GREEN, ZINC CHLORIDE SALT
METHYL GREEN CRYSTAL VIOLET-FREE
FERRIC NITRATE NONAHYDRATE ACS GRADE
METHYL GREEN, ZINC CHLORIDE SALT, CERTIF IED
Methyl Green, zinc chloride salt, for analysis
Methyl Green, zinc chloride salt,pure,certified
Methyl Green, zinc chloride salt, certified, pure
Methyl Green, zinc chloride salt,pure,high purity biological stain
Methyl Green, zinc chloride salt, high purity biological stain, pure
Methyl green, crystal violet free, (4-((4-(Dimethylamino)phenyl)(4-(dimethyliminio)cyclohexa-2,5-dien-1-ylidene)methyl)-N-ethyl-N,N-dimethylanilinium bromide chloride zinc chloride salt
Benzenaminium, 4-4-(dimethylamino)phenyl4-(dimethyliminio)-2,5-cyclohexadien-1-ylidenemethyl-N-ethyl-N,N-dimethyl-, bromide chloride, compd. with zinc chloride (ZnCl2) | [EINECS(EC#)]
230-415-4 | [Molecular Formula]
C27H35BrCl3N3Zn | [MDL Number]
MFCD00151094 | [MOL File]
7114-03-6.mol | [Molecular Weight]
653.24 |
| Chemical Properties | Back Directory | [Appearance]
maroon to reddish-brown crystals | [Melting point ]
>300 °C(lit.)
| [bulk density]
300kg/m3 | [storage temp. ]
Store at RT. | [solubility ]
Soluble in water, ethanol; insoluble in xylene | [Colour Index ]
42590 | [form ]
Solid | [color ]
Green | [PH]
4.6 (20°C, 10g/L in H2O) | [PH Range]
Yellow (0.1) to greenish-blue (2.3) | [Stability:]
Stable. Incompatible with strong oxidizing agents. | [Water Solubility ]
70 g/L (20 ºC) | [ε(extinction coefficient)]
≥14000 at 311-317 in water at 0.005g/L
≥50000 at 629-635 in water at 0.005g/L
≥9000 at 420-426 in water at 0.005g/L | [λmax]
629 nm, 423 nm | [Biological Applications]
Detecting phosphates,polyphosphates,tocopherol,tocopherol acetate | [Major Application]
diagnostic assay manufacturing
hematology
histology | [InChI]
1S/C27H35N3.4ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4 | [InChIKey]
ZKOGXCSOLMZRAZ-UHFFFAOYSA-J |
| Hazard Information | Back Directory | [Chemical Properties]
maroon to reddish-brown crystals | [Uses]
Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently. | [Biological Activity]
Marker dye for DNA in acid and neutral media.
Methyl green is mainly used as a DNA stain in histochemistry. at low concentrations it binds selectively to AT-rich regions of native DNA. However at high concentrations it is present at all available DNA sites. | [Purification Methods]
Crystallise the dye from hot water. [Beilstein 13 IV 2286.] |
| Questions And Answer | Back Directory | [Description]
Methyl Green, Zinc chloride salt (chemical formula: C27H35Cl4N3Zn) is the zinc chloride salt form of Methyl green. Methyl green is a cationic or positive charged stain. Since it can binds selectively to the AT-rich regions of native DNA, it is mainly used for the DNA and RNA staining in the cell nuclei. Methyl Green, Zinc chloride Salt, as a CERTISTAIN® dry dye for DNA staining, it has many application: used as a tracking dye in acid buffers for electrophoresis; used to demonstrate DNA and RNA in tissues sections in the Methyl Green-Pyronin method; used as an exclusion viability stain for cells. In addition, it can also be used in fluorimetric assay and flow cytometry.
| [Indicator]
Preparation of a methyl green zinc chloride double salt solution: 0.1 g of methyl green zinc chloride double salt in 100 mL of water. This solution is used as an acid-base indicator; at a pH of 0.1 to 2.3, the colour changes from yellow to blue. | [References]
http://www.sigmaaldrich.com/catalog/product/sigma/67060?lang=zh®ion=CN
https://pubchem.ncbi.nlm.nih.gov/compound/3084002#section=Canonical-SMILES
https://en.wikipedia.org/wiki/Methyl_green
http://www.abcam.com/methyl-green-zinc-chloride-salt-ab146287.html
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J & K SCIENTIFIC LTD.
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18210857532 18210857532 |
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https://www.jkchemical.com |
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Energy Chemical
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400-0056266 |
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www.energy-chemical.com |
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Maya High Purity Chemicals
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+86 (573) 82222445 (0)18006601000 452520369 |
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www.chemicalbook.com/ShowSupplierProductsList15221/0_EN.htm |
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Spectrum Chemical Manufacturing Corp.
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021-021-021-67601398-809-809-809 15221380277 |
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