| Identification | More | [Name]
6-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER | [CAS]
92557-81-8 | [Synonyms]
6-CARBOXYFLUORESCEIN-NHS
6-CARBOXYFLUORESCEIN-N-HYDROXYSUCCINIMIDE ESTER
6-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER
6-CARBOXYFLUORESCEIN SE
6-CARBOXYFLUORESCEIN SUCCINIMIDE ESTER
6-CARBOXYFLUORESCEIN, SUCCINIMIDYL ESTER
6-FAM-NHS
6-FAM, SE
6-CARBOXYFLUORESCEIN, SUCCINIMIDYL*ESTER (SINGLE IS
6-CARBOXYFLUORESCEIN,SUCCINIMIDYL ESTER,6-FAM,SE 98%
6-CARBOXYFLUORESCEIN N-HYDROXYSUCCINIMIDE
6-Carboxyfluorescein N-succinimidyl ester, 6-FAM, SE | [Molecular Formula]
C25H15NO9 | [MDL Number]
MFCD00077323 | [Molecular Weight]
473.39 | [MOL File]
92557-81-8.mol |
| Chemical Properties | Back Directory | [Melting point ]
>168°C (dec.) | [Boiling point ]
788.1±70.0 °C(Predicted) | [density ]
1.74±0.1 g/cm3(Predicted) | [storage temp. ]
−20°C
| [solubility ]
Soluble in N,N-dimethylformamide,dimethyl sulfoxide | [form ]
Solid | [pka]
9.31 ± 0.20, most acidic, temperature:25 °C | [color ]
Orange solid | [Appearance]
Yellow solid | [ex/em]
492/517 nm | [ε(extinction coefficient)]
74,000 L⋅mol−1⋅cm−1 | [λmax]
496 nm (Buffer pH9) | [Φ(quantum yield)]
0.93 | [Stability:]
Moisture Sensitive | [Biological Applications]
Analyzing
aminoglycosides;
analyzing/determining gabapentin;
detecting/analyzing baclofen in human plasma at
micromolar level;
determining phosphorus-containing
amino acid herbicides;
multiplex genetic analysis;
visualizing/detecting proteins; for synthesizing
vancomycin analogs,
vasopressin analogs;
as a substrate for measuring kinase activity, phosphatase
activity. | [InChI]
InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2 | [InChIKey]
VDABVNMGKGUPEY-UHFFFAOYSA-N | [SMILES]
C12(C3=C(C=C(O)C=C3)OC3=C1C=CC(O)=C3)C1=C(C=CC(C(ON3C(=O)CCC3=O)=O)=C1)C(=O)O2 | [CAS DataBase Reference]
92557-81-8(CAS DataBase Reference) |
| Hazard Information | Back Directory | [Description]
Fluorescein (FAM) is a bright fluorophore that has found an extremely wide application in life science research. The fluorophore is hydrophilic and its derivatives possess good solubility in aqueous buffers.
Fluorescein is compatible with a wide variety of fluorescent instrumentation - most instruments are equipped with FAM channel filters.
This product contains the pure 6-isomer of fluorescein. | [Chemical Properties]
Yellow to orange powder | [Uses]
(6-FAM-SE) 6-Carboxyfluorescein-N-hydroxysuccinimide Ester is a commonly used fluorescent reagent for the labelling of peptides, proteins and nucleotides. | [Uses]
6-Carboxyfluorescein-N-hydroxysuccinimide Ester is a commonly used fluorescent reagent for the labelling of peptides, proteins and nucleotides. | [Uses]
Intracellular pH probe. Fluorescent label for amines and alcohols. | [Definition]
ChEBI: 6-carboxyfluorescein succinimidyl ester is an N-hydroxysuccinimide ester derived from 6-carboxyfluorescein. A fluorescent cell staining dye, it is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably to intracellular lysine residues. It has a role as a fluorochrome. It is functionally related to a fluorescein (lactone form). | [Abs/Em Maxima]
492/517 nm | [Fluoresene quantum yield]
0.93 | [Extinction Coefficient]
74000 | [CF260]
0.22 | [CF280]
0.17 |
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