DPN I

81295-14-9

CAS-Nr.: 81295-14-9

Englisch Name:: DPN I

Synonyma:: DPN I;DPN I RESTRICTION ENZYME;restriction endonuclease Dpn I;RESTRICTION ENDONUCLEASES DPN I;dpn i from diplococcus pneumoniae;restriction endonuclease dpn I from*diplococcus P;RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE

CBNumber:: CB0256555

Summenformel:

Molgewicht:: 0

MOL-Datei:: Mol file

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Eigenschaften Sicherheit Verwendungswort Produktionsfirma 60

DPN I Eigenschaften

storage temp.: -20°C

Aggregatzustand: buffered aqueous glycerol solution

Sicherheit

RIDADR	UN3373 - class 6.2 Biological substance, Category B
WGK Germany	3

DPN I Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR.

Allgemeine Beschreibung

Isoschizomers
The enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.
Methylation sensitivity
The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.
Typical ligation and recutting assay
Dpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl₂, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.

DPN I Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte

DPN I Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global( 60)Lieferanten

Firmenname	Telefon	E-Mail	Land	Produktkatalog	Edge Rate
Career Henan Chemica Co	+86-0371-86658258 15093356674;	laboratory@coreychem.com	China	30255	58
3B Pharmachem (Wuhan) International Co.,Ltd.	821-50328103-801 18930552037	3bsc@sina.com	China	15848	69
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80
Shanghai Kanglang Biotechnology Co., Ltd.	021-61998208 18217752821	sales@klbio.cn	China	9617	58
Wuhan Chemstan Biotechnology Co., Ltd.	15926423062	422450190@qq.com	China	10132	58
Shanghai Yuchun Biotechnology Co., Ltd	021-021-52273538 17321241593	1503472510@qq.com	China	5250	58

81295-14-9()Verwandte Suche:

Adenosin-5'-(trihydrogendiphosphat), 5'-5'-Ester mit 3-Carbamoyl-1-α-D-ribofuranosylpyridinium-at Tris(2,2,6,6-tetramethylheptan-3,5-dionato-O,O')dysprosium Benzylisocyanid (p-Toluolsulfonyl)methylisocyanid N-Butylisocyanid 1,1,3,3-Tetramethylbutylisocyanat Phenylselenol Kupfer(II)-4-oxopent-2-en-2-olat 2-Methylpropylisocyanid Methylisocyanacetat (Pentan-2,4-dionato-O,O')silber Aluminiumtris(2,4-pentandionato-O,O') Tris(2,2,6,6-tetramethylheptan-3,5-dionato-O,O')europium (Ethylenbis(nitrilomethylidyn)-2,2'-diphenolato)cobalt(II) Ethylisocyanacetat Tris(pentan-2,4-dionato)eisen(II) Adenosin-5'-(trihydrogen-diphosphat), 5'-5'-Ester mit 3-(Aminocarbonyl)-1-β-D-ribofuranosylpyridiniumhydroxid, inneres Salz, Monolithiumsalz Diprenorphin

RESTRICTION ENDONUCLEASES DPN I
DPN I
DPN I RESTRICTION ENZYME
restriction endonuclease dpn I from*diplococcus P
RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE
dpn i from diplococcus pneumoniae
restriction endonuclease Dpn I
81295-14-9
Restriction Endonucleases
Molecular Biology Enzymes
Molecular Biology
BioChemical
Restriction EnzymesEnzyme Class Index
3.1.x.x Acting on esters
3.x.x.x Hydrolases
Molecular Biology
Molecular Biology Enzymes
Molecular Biology Tested
Restriction EndonucleasesApplication Index