- CAS No.
- Chemical Name:
- MES monohydrate
- MES HYDRATE;MES.H2O;MORPHOLINOETHANE SULFONIC ACID MONOHYDRATE;2-(N-MORPHOLINO)ETHANESULFONIC ACID HYDRATE;4-MORPHOLINEETHANESULFONIC ACID MONOHYDRATE;MES-H;MES-HO;MES)MES Monohyd;MES MONOHYDRATE;MES monohydrate，MES
- Molecular Formula:
- Molecular Weight:
- MDL Number:
- MOL File:
- MSDS File:
|Melting point||>300 °C(lit.)|
|storage temp.||Sealed in dry,Room Temperature|
|solubility||H2O: 0.5 M at 20 °C, clear|
|form||Powder, Crystals or Flakes|
|color||White to pale yellow and/or orange|
|PH||2.5-4.0 (25℃, 0.5M in H2O)|
|PH Range||5.5 - 6.7|
|Water Solubility||Soluble in water.|
λ: 260 nm Amax: 0.025
λ: 280 nm Amax: 0.020
|CAS DataBase Reference||145224-94-8(CAS DataBase Reference)|
Risk and Safety Statements
MES monohydrate price More Price(98)
|Manufacturer||Product number||Product description||CAS number||Packaging||Price||Updated||Buy|
|Sigma-Aldrich||69889||MES monohydrate BioUltra, for molecular biology, ≥99.5% (T)||145224-94-8||10g||$93.6||2023-06-20||Buy|
|Sigma-Aldrich||69889||MES monohydrate BioUltra, for molecular biology, ≥99.5% (T)||145224-94-8||50g||$297||2023-06-20||Buy|
|Sigma-Aldrich||RES0113M-A7||MES monohydrate PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production, suitable for cell culture||145224-94-8||1pkg||$3045||2022-05-15||Buy|
|Alfa Aesar||A16104||MES monohydrate, 98%||145224-94-8||25g||$42.7||2023-06-20||Buy|
|Alfa Aesar||A16104||MES monohydrate, 98%||145224-94-8||100g||$101||2023-06-20||Buy|
MES monohydrate Chemical Properties,Uses,Production
MES Monohydrate is a zwitterionic buffer that is not absorbed through cell membranes and is virtually transparent in UV light. MES is a buffering agent used in many biological and biochemical applications. It is also used as a running buffer for denaturing gel electrophoresis. The characteristics of low UV absorptivity, minimal reactivity, stable pH and solubility in water allow MES Monohydrate to be used as a Good's buffer.
white crystalline powder
MES Monohydrate has been used:
- To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay
- As a buffering agent to stabilize enzymatic solutions
- As a component of PAGE running buffer
MES monohydrate is a biological buffer often referred to as Good?s buffer. It is useful for cell culture media and protein based buffer formulations to maintain a stable environment in solution. It is also used in the purification bioprocesses of antibodies, peptides, proteins, blood components and growth factors. It is a useful resolving agent for very small proteins on bis-tris gels. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical applications.
MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.
MES free acid or MES free acid monohydrate can be mixed with MES sodium salt to acquire desired pH. Alternatively, MES free acid or MES free acid monohydrate can be titrated with sodium hydroxide to attain desired pH.
MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.
MES Monohydrate has been used:
To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay.
As a buffering agent to stabilize enzymatic solutions.
As a component of PAGE running buffer.
buffer substance MES
Preparation and handling
A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.
Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.
MES monohydrate Preparation Products And Raw materials
|Suzhou Genemill Biotechnology Co.,Ltdemail@example.com||China||39||58|
|Hebei Yanxi Chemical Co., Ltd.||+firstname.lastname@example.org||China||5948||58|
|Hubei Xindesheng Material Technology Co., Ltd.||+email@example.com||China||174||58|
|Henan Bao Enluo International TradeCo.，LTD||+86-17331933971 +firstname.lastname@example.org||China||2506||58|
|Henan DaKen Chemical CO.,LTD.||+email@example.com||China||14736||58|
|Henan Tianfu Chemical Co.,Ltd.||+86-0371-55170693 +firstname.lastname@example.org||China||21722||55|
|Anhui Royal Chemical Co., Ltd.||+86-25-86655873 +email@example.com||China||535||55|
|Hefei TNJ Chemical Industry Co.,Ltd.||+86-0551-65418679 +firstname.lastname@example.org||China||2990||55|
|career henan chemical email@example.com||China||29922||58|
|Accela ChemBio Inc.||(+1)-firstname.lastname@example.org||United States||19965||58|
- What is Good's buffer？
- MES belongs to Good's buffer. They are well-performing buffers and are often used in biological research.
- Sep 13，2023
View Lastest Price from MES monohydrate manufacturers
|Image||Update time||Product||Price||Min. Order||Purity||Supply Ability||Manufacturer|
|US $0.00 / kg||1kg||99.5%||1000||Suzhou Genemill Biotechnology Co.,Ltd|
|US $0.00 / kg||1kg||0.99||20 tons||Hebei Yanxi Chemical Co., Ltd.|
|US $0.00-0.00 / kg||1kg||99%||50000kg||Hebei Yibangte Import and Export Co. , Ltd.|
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