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Propidium iodide

Chemical Name:
Propidium iodide
PI;Propidium iodide solution;Nsc169541;PI solution;-Cellstain-Pi;PI(Powder form);PROPIDIUM IODIDE;Propidium diiodide;Propidium Iodide(PI);PI Staining Solution
Molecular Formula:
Molecular Weight:
MDL Number:
MOL File:
MSDS File:
Modify Date:2022-12-21 16:56:50

Propidium iodide Properties

Melting point 220-225 °C (dec.) (lit.)
Density 1.5072 (estimate)
storage temp. 2-8°C
solubility Difficult to mix.
form Flaky Powder
color Dark red
Water Solubility Soluble in water at 1mg/ml
Sensitive Light Sensitive & Hygroscopic
λmax 493 nm, 535 nm
BRN 3843838
Biological Applications Detecting nucleic acids,cancer cells,spores,activity of hydrolase,microorganisms; apoptosis assay; measuring beta-amyloid in Alzheimer’s disease (AD); treating diseases associated with androgens,cancer
Major Application Electroluminescent displays; photoresists
CAS DataBase Reference 25535-16-4(CAS DataBase Reference)


Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
Signal word  Warning
Hazard statements  H341
Precautionary statements  P201-P202-P280-P308+P313-P405-P501
Hazard Codes  Xi,Xn
Risk Statements  36/37/38-68-67-48/20/22-40-36/38-22-20-63
Safety Statements  26-36-22-36/37
WGK Germany  3
RTECS  SF7949600
HS Code  29339900

Propidium iodide price More Price(42)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 537059 Propidium Iodide Membrane impermeable DNA intercalator. 25535-16-4 50mg $114 2023-01-07 Buy
Sigma-Aldrich 79214 Bacteria stain propidium iodide solution 1?mg/mL in H2O, ready to use solution, 100 assays 25535-16-4 4X25μL $133 2023-01-07 Buy
Sigma-Aldrich 537059 Propidium Iodide - CAS 25535-16-4 - Calbiochem Membrane impermeable DNA intercalator. 25535-16-4 1g $1700 2023-01-07 Buy
Sigma-Aldrich S7109 Propidium Iodide Solution 25535-16-4 1ML $191 2022-05-15 Buy
Alfa Aesar J66764 Propidium iodide 25535-16-4 100mg $169.05 2023-01-06 Buy
Product number Packaging Price Buy
537059 50mg $114 Buy
79214 4X25μL $133 Buy
537059 1g $1700 Buy
S7109 1ML $191 Buy
J66764 100mg $169.05 Buy

Propidium iodide Chemical Properties,Uses,Production


Propidium iodide (PI) is membrane impermeant and generally excluded from viable cells. However, it can easily penetrate dead or damaged cells and as such is commonly used for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques. It binds to DNA and RNA by intercalating between the bases. PI is excited at 488-535 nm with an emission maximum of 617 nm. This probe is suitable for fluorescence microscopy and flow cytometry.

Chemical Properties

Dark Red Solid


Propidium Iodide is a phenanthridine compound, a class of compounds with reported activity against several species of trypanosomes. Propidium iodide may be used in flow cytometry to evaluate cell viab ility when used with other dyes that stain viable cells or cells that are early in the apoptosis process.


Used as fluorescent stain for nucleic acids,and used in flow cytometry to evaluate cell viability




1 mg/mL in water


Propidium iodide is the most common red-fluorescent nuclear stain. It can also be excited using excitation filters appropriate for green-fluorescent dyes.

General Description

Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1;

Biochem/physiol Actions

Primary TargetMembrane impermeable DNA intercalator

Purification Methods

It crystallises as red crystals from H2O containing a little KI. It fluoresces strongly with nucleic acids. [Watkins J Chem Soc 3064 1952, Beilstein 22 III/IV 5519.] TOXIC.

Propidium iodide Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Propidium iodide manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Propidium iodide pictures 2023-02-02 Propidium iodide
US $0.00-0.00 / KG 1KG 99% 500000kg Hebei Guanlang Biotechnology Co., Ltd.
Propidium iodide pictures 2019-09-02 Propidium iodide
US $7.00 / KG 1KG 99% JD 432 Career Henan Chemical Co
Propidium iodide pictures 2019-07-14 Propidium iodide
US $1.00 / KG 1KG 99% 25kg Career Henan Chemical Co
  • Propidium iodide pictures
  • Propidium iodide
  • US $0.00-0.00 / KG
  • 99%
  • Hebei Guanlang Biotechnology Co., Ltd.
3,8-DIAMINO-5-(3-DIETHYLAMINOPROPYL)-6-PHENYLPHENANTHRIDINIUM IODIDE METHIODIDE 3,8-DIAMINO-5-(3-DIETHYL-AMINOPROPYL)-6-PHENYLPHENANTHRIDIUM IODIDE METHIODIDE 3,8-DIAMINO-5-(3-[DIETHYLMETHYLAMMONIO])-6-PHENYLPHENAN-THRIDINIUM DIIODIDE 3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM 3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM DIIODIDE 3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenyl-phenanthridiniudii 3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenylphenanthridiniumdiiod 3,8-diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridiniumiodidemethiodi PHENANTHRIDINIUM, 3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYL-, DIIODIDE 3,8-Diamino-5-[3-(diethylmethylamino)propyl]-6-phenylphenanthridium,diiodide 3,8-Diamino-5-[3-(diethylmethylamino)propyl]-6-phenylphenanthridium,diiodide,aqueoussolution Propidium diiodide PROPIDIUM IODIDE 95-98% PROPIDIUM IODIDE, FOR FLUORESCENCE PROPIDIUM IODIDE 95% -Cellstain-PiSolution -Cellstain-Pi PROPIDIUM IODIDE RED POWDER 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium, diiodide, 3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide Nsc169541 3,8-DiaMino-5-(diethylaMinopropyl)-6-phenylphenanthridiniuM iodide Methiodide PROPIDIUM IODIDERESEARCH GRADE Propidium iodide,3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide [3-(3,8-DiaMino-6-phenyl-5-phenanthridinio)propyl]diethylMethylaMMoniuM Diio 3,8-DiaMino-5-[3-(diethylMethylaMMonio)propyl]-6-phenylphenanthridiniuM Iodide 3,8-diaMino-5-(3-(diethyl(Methyl)aMMonio)propyl)-6-phenylphenanthridin-5-iuM iodide 3,8-Diamino-5-(3-(diethyl(methyl) -6-phenylphenanthridin-5-ium iodide Bacteria stain propidium iodide solution Propidium Iodide DMSO Solution(1mg/ml) Propidium Iodide, For HPLC analysis Ammonium, [3-(3,8-diamino-6-phenyl-5-phenanthridinio)propyl]diethylmethyl-, Diiodide 3,8-diamino-5-(diethylmethylaminopropyl)-6-phenylphenanthridinium Diiodide PROPIDIUM IODIDE Propidium Iodide - CAS 25535-16-4 - Calbiochem Propidium Iodide Solution - CAS 25535-16-4 - Calbiochem 3,8-diamino-5-(3-diethylaminopropyl)-6-phenylp Propidium iodide *10 mM aqueous solution* Propidium iodide *CAS 25535-16-4* PI solution Propidium Iodide(PI) Cellstain PI Solution (Propidium Iodide 1mg/ml H2O Solution) 3,8-Diamino-5-(3-diethylaminopropyl)-6-p Propidium iodide, 95%, Acros Organics^t PI [Propidium iodide] Propidium iodide USP/EP/BP PI Staining Solution PI(Powder form) Propidium iodide, 94%, suitable for cell culture Propidium iodide, solution 1.0 mg/ml in water Propidium Iodide (PI) for molecular biology, 95% Propidium Iodide Solution (1mg/ml in H2O) Propidium iodide, suitable for cell culture PI Propidium iodide solution 25535-16-4 C27H34N42I C27H34N4I2