D-荧光素钾盐
中文名称 | D-荧光素钾盐 |
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中文同义词 | (S)-4,5-二氢-2-(6-羟基苯并噻唑-2-基)噻唑-4-甲酸钾盐;D-荧光素钾;荧光素钾;D-荧光素钾盐,D-虫荧光素钾盐;D-虫荧光素钾盐;D-荧光素钾盐;D-荧光素钾盐, ≥98.0% (HPLC);D-荧光素钾盐POTASSIUM (S)-2-(6-HYDROXYBENZO[D]THIAZOL-2-YL)-4,5-DIHYDROTHIAZOLE-4-CARBOXYLATE |
英文名称 | D-Luciferin potassium salt |
英文同义词 | D-(-)-2-[6'-HYDROXY-2'-BENZOTHIAZOLYL]DELTA-THIAZOLINE-4-CARBOXYLIC ACID POTASSIUM SALT;D-LUCIFERIN FIREFLY, POTASSIUM SALT;D-LUCIFERIN, MONOPOTASSIUM SALT;D-LUCIFERIN POTASSIUM SALT;BEETLE LUCIFERIN, POTASSIUM SALT;4,5-DIHYDRO-2-(6-HYDROXY-2-BENZOTHIAZOLYL)-4-THIAZOLECARBOXYLIC ACID POTASSIUM SALT;D-LUCIFERIN K SALT;D -LUCIFERIN SYNTHETIC POTASSIUM SALT |
CAS号 | 115144-35-9 |
分子式 | C11H9KN2O3S2 |
分子量 | 320.42 |
EINECS号 | 1592732-453-0 |
相关类别 | 生物试剂;生化试剂;生物发光底物;发光化合物;医药中间体;化学发光;荧光染料;化学试剂;其他生化试剂;医用原料;标准品 -中药标准品;原料药;PCR试剂盒-荧光定量PCR试剂盒;活性小分子库;试剂盒-细胞分析试剂盒;通用生化试剂-有机酸;酶抑制剂;智尚化工中间体;医药原料;PCR试剂盒-活体成像用试剂系列;原料;标准品;调节剂;芳烃;精细化工;其他类 |
Mol文件 | 115144-35-9.mol |
结构式 |
D-荧光素钾盐 性质
储存条件 | Inert atmosphere,Store in freezer, under -20°C |
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溶解度 | DMF:16.7(Max Conc. mg/mL);52.45(Max Conc. mM) DMSO:7.5(Max Conc. mg/mL);23.55(Max Conc. mM) Ethanol:0.25(Max Conc. mg/mL);0.79(Max Conc. mM) PBS (pH 7.2):10.0(Max Conc. mg/mL);0.31(Max Conc. mM) Water:32.0(Max Conc. mg/mL);100.5(Max Conc. mM) |
形态 | 结晶固体 |
颜色 | 浅黄色 |
BRN | 5416262 |
InChI | InChI=1/C11H8N2O3S2.K.H/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;;/h1-3,7,14H,4H2,(H,15,16);;/t7-;;/s3 |
InChIKey | UMBKGTQQGYPQBE-OGFXRTJISA-M |
SMILES | C([C@H]1CSC(C2=NC3C=CC(O)=CC=3S2)=N1)(=O)O.[KH] |&1:1,r| |
CAS 数据库 | 115144-35-9(CAS DataBase Reference) |
D-荧光素钾盐是荧光素酶的水溶性底物,存在于多种发光生物体中。在ATP和荧光素酶的催化作用下,D-荧光素钾被氧化,产生蓝绿色的光(560nm),当底物过量时,产生的光量子数与荧光素酶的浓度呈正相关。编码荧光素酶的Luc基因是植物、哺乳动物细胞的常用报告基因。由于没有背景干扰,因此可以很容易地检测出低至0.02 pg 水平的荧光素酶。
D-荧光素钾盐是一类在生物体中发现的能引起生物发光的杂环化合物,如萤火虫。在ATP存在下,萤光素酶将其氧化脱羧后会发光。化学研究中可用于荧光素酶的基板。
D-Luciferin是萤火虫荧光素酶的底物。
D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typical yellowgreen light of fireflies.The present review covers the synthesis of D-luciferin and derivatives or analogues that are substrates or inhibitors of the luciferase from the American firefly
Photinus pyralis
, the enzyme more frequently used in techniques of in vitro and optical imaging.
D-Luciferin exhibits a decrease in the measured K
m
in PC3M-Luc cell lysates with a K
m
of 34 μM.
Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and D-luciferin as a substrate is currently the most widely employed technique. The total signal intensity is plotted against the time after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals at fixed time points (5, 10, 15, and 20 min) after D-luciferin injection are determined as alternatives to the peak signal. The signal in a given time-intensity curve is normalized for the peak signal in the curve to represent the pattern of temporal changes after D-luciferin injection.
Inject with 10 μL of D-luciferin (intraperitoneally or intravenously) stock solution per gram of body weight: normally ~200 μL for a 20 g mouse for a standard 150 mg/kg injection.
Thaw D-Luciferin (either Potassium or Sodium Salt) at room temperature and dissolve in dPBS (no calcium or magnesium) to a final concentration of 15 mg/mL. Pre-wet a 0.22 μm filter by drawing through 5-10 mL of sterile H
2
O and discard water. Sterilize the D-Luciferin solution through the prepared 0.22 μm syringe filter.
安全信息
WGK Germany | 3 |
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海关编码 | 29342000 |
更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
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2024/08/19 | HY-12591B | D-Luciferin potassium | 5 mg | 312元 | |
2024/08/19 | HY-12591B | D-荧光素钾盐 D-Luciferin potassium | 115144-35-9 | 10mg | 500元 |