ChemicalBook >> CAS DataBase List >>Fluorexon


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Modify Date:2023-02-08 10:53:32

Fluorexon Properties

Melting point 200 °C
Boiling point 662.7°C (rough estimate)
Density 1.4388 (rough estimate)
refractive index 1.5800 (estimate)
storage temp. Keep in dark place,Sealed in dry,Room Temperature
solubility H2O: soluble
form Powder
pka 6.67(at 25℃)
color Yellow-orange to orange-brown
PH Range Weak green ' uorescence (6.0) to strong green ' uorescence (7.2)
Water Solubility insoluble
λmax 494nm
Stability Stable. Incompatible with strong oxidizing agents.
Biological Applications Calcium indicator; fluoride indicator; iron indicator; mercury indicator; detecting nucleic acids,proteins; treating osteoporosis; drug delivery systems
Major Application Chemical-mechanical polishing, stabilizing liposomes, cell motility assay, detecting nucleic acids, virus, screening safety of gastric mucosa, MDR inhibitors, delivery, imaging atherosclerotic plaque
CAS DataBase Reference 1461-15-0(CAS DataBase Reference)
EPA Substance Registry System Glycine, N,N'-[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl)bis(methylene)]bis[N-(carboxymethyl)- (1461-15-0)


Risk and Safety Statements

Hazard Codes  Xn
Risk Statements  20/21/22-36/37/38
Safety Statements  22-24/25-36/37/39-26
WGK Germany  3
HS Code  32041900

Fluorexon price More Price(36)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 1.02315 Calcein indicator for metal determination 1461-15-0 5G $211 2022-05-15 Buy
Cayman Chemical 16221 Calcein 1461-15-0 1g $28 2023-01-06 Buy
Cayman Chemical 16221 Calcein 1461-15-0 5g $49 2023-01-06 Buy
Cayman Chemical 16221 Calcein 1461-15-0 10g $82 2023-01-06 Buy
Cayman Chemical 16221 Calcein 1461-15-0 25g $150 2023-01-06 Buy
Product number Packaging Price Buy
1.02315 5G $211 Buy
16221 1g $28 Buy
16221 5g $49 Buy
16221 10g $82 Buy
16221 25g $150 Buy

Fluorexon Chemical Properties,Uses,Production

Chemical Properties

orange crystals


Calcein could be useful for magnetic resonance imaging-guided sonodynamic and nitric oxide combination therapy.


Calcein is a anthene that is commonly used for the fluorometric determination of calcium in solution. As this form of calcein is not membrane permeable, it can be used in assays that evaluate membrane integrity. Calcein can also be used as a fluorescent indicator for fluoride, iron, and mercury. [Cayman Chemical]


Calcein is used for determination of Ca in biological systems. Indicator for the fluorimetric determination of calcium in presence of Mg by complexometric titration.

Biological Activity

Calcein is a fluorescent dye and self-extinguishing probe that can be used as an indicator of lipid vesicle leakage, as well as a complex indicator for EDTA calcium titration and fluorescence determination of calcium.

in vitro

Calcein accumulates intracellularly in coelomocytes incubated in coelomic fluid or ISO-EDTA. Coelomocytes incubated in CF show an increase in the calcein fluorescence intensity compared to the control.

in vitro

Calcein accumulates intracellularly in coelomocytes incubated in coelomic fluid or ISO-EDTA. Coelomocytes incubated in CF show an increase in the calcein fluorescence intensity compared to the control.

Purification Methods

Dissolve it in distilled H2O and acidify with dilute HCl to pH 3.5. Filter off the solid acid and wash it well with H2O. Redissolve ca 10g in 300mL H2O containing 12g of NaOAc. Precipitate it again by adding HCl, filtering and washing with H2O. Add the solid to 200mL of EtOH stir for 1hour and filter. Repeat the EtOH wash and dry the bright yellow solid in a vacuum. This acid decomposes on heating at ca 180o. See below for the preparation of the Na salt. [Diehl & Ellingboe Anal Chem 28 882 1956]. Altenatively dissolve it in H2O and acidify with 3N HCl to pH 3.5. Collect the solid and wash it with H2O. The air-dried precipitate is extracted with 70% aqueous EtOH, filtered hot and cooled slowly. Fine yellow needles of the acid crystallise out; they are filtered off and dissolved in the minimum quantity of 0.01N NaOH and re-precipitated by adding N HCl to pH 3.5. It is then recrystallised from 70% aqueous EtOH (3x). The final product (acid) is dried at 80o in a vacuum for 24hours, m >300o(dec). It contains one molecule of water per molecule of acid (C30H36N4O13.H2O). The product is pure as revealed by electrophoresis at pH 5.6 and 8.6, and by TLC in i-BuOH/i-PrOH/AcOH/H2O (60:60:5:5 by volume) or i-PrOH or pH 8.0 borate buffer. [Wallach et al. Anal Chem 31 456 1959.] The Na salt is prepared by dissolving the pure acid in H2O containing 2 mols of NaOH per mol of acid reagent and lyophilising. It complexes with Ca and Mg ions. [Beilstein 19 III/IV 4338.]

Fluorexon Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 356)Suppliers
Supplier Tel Email Country ProdList Advantage
Henan DaKen Chemical CO.,LTD.
+86-371-66670886 China 17709 58
Hangzhou FandaChem Co.,Ltd.
+8615858145714 China 9375 55
career henan chemical co
+86-0371-86658258 China 29922 58
Chongqing Chemdad Co., Ltd
86-13650506873 China 39915 58
Alchem Pharmtech,Inc.
8485655694 United States 63711 58
Shenyang Simchoice Chemical Co.,Ltd
+86-024-23769576 +86-15040101888 China 253 58
+8618523575427 China 47427 58
Zhuozhou Wenxi import and Export Co., Ltd
+8613111626072 CHINA 13170 58
Career Henan Chemica Co
+86-0371-86658258 China 30262 58
Hefei TNJ Chemical Industry Co.,Ltd.
0551-65418671 CHINA 34572 58

View Lastest Price from Fluorexon manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Fluorexon pictures 2023-02-09 Fluorexon
US $0.00 / KG 1KG 98%min 30tons/month WUHAN FORTUNA CHEMICAL CO., LTD
Fluorexon pictures 2021-08-11 Fluorexon
US $15.00-10.00 / KG 1KG 99%+ HPLC Monthly supply of 1 ton Zhuozhou Wenxi import and Export Co., Ltd
Fluorexon pictures 2019-07-06 Fluorexon
US $1.00 / KG 1G 99% 100KG Career Henan Chemical Co
  • Fluorexon pictures
  • Fluorexon
  • US $0.00 / KG
  • 98%min
  • Fluorexon pictures
  • Fluorexon
  • US $15.00-10.00 / KG
  • 99%+ HPLC
  • Zhuozhou Wenxi import and Export Co., Ltd
  • Fluorexon pictures
  • Fluorexon
  • US $1.00 / KG
  • 99%
  • Career Henan Chemical Co
BIS[N,N-BIS(CARBOXYMETHYL)AMINOMETHYL]FLUORESCEIN CALCEINE CALCEIN, INDICATOR FLUORESCEIN-2,7-BIS-METHYLIMINODIACETIC SODIUM SALT FLUORESCEIN COMPLEXON FLUORESCEIN COMPLEXONE FLUORESCEIN-METHYLENE-IMINODIACETIC ACID FLUOREXON FLUOREXONE FLUREXONE LABOTEST-BB LT00159821 2,7-BLS[BIS(CARBOXYMETHYL)AMINOMETHYL]-FLUORESCEIN 3,3'-BIS[N,N-BIS(CARBOXYMETHYL)AMINOMETHYL]FLUORESCEIN 3,6-DIHYDROXY-2,4-BIS-N,N'-DI-(CARBOXYMETHYL)-AMINOMETHYL FURAN FLUOREXON, INDICATOR GRADE CALCEIN INDICATOR FOR THE COMPLEXOMETRY CalceineIndicatorGr CalceineIndicatorGr(FluoresceinComplexone) CalceineIndicatorGr-(FluoresceinComplexon) FLUORESCEIN COMPLEXONE GR Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein~Fluorexon Oftasceine Glycine, N,N-(3,6-dihydroxy-3-oxospiroisobenzofuran-1(3H),9-9Hxanthene-2,7-diyl)bis(methylene)bisN-(carboxymethyl)- CALCEIN(RG) Fluorescein di-(methylimino diacetic acid) Calcein(Fluorexone) Calcein [for Fluorometric Determination of Ca] Fluorescein-bis(methyliminodiacetic acid), Fluorexon, Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein, Calcein, Fluorescein Complexon N,N'-[[3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-(carboxymethyl)glycine] Calcein,Bis[N,Nbis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon Calcein, indicator for metal titration Bis-[di(carboxyMethylaMinoMethyl)]flurescein sodiuM salt Fluorescein-2,7-bis-MethylaMinodiacetic acid sodiuM salt CALCEIN HYDRATE: FOR FLUOROMETRIC DETERMINATION OF CA CALCEIN INDICATOR FOR METAL DETERMINATIO 7’-diyl)bis(methylene)]bis[n-(carboxymethyl)--2 glycine,n,n’-[(3’,6’-dihydroxy-3-oxospiro[isobenzofuran-1(3h),9’-[9h]xanthene] N,N’-[(3’,6’-dihydroxy-3-oxospiro(isobenzofuran-1(3H),9’-(9H)xanthene)-2’,7’-diyl)bis(methyGlycine Fluorescein-bis(methyliminodiacetic acid) N,N'-[[3' oftasccine 2,2',2'',2'''-(((3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-2',7'-diyl)bis(Methylene))bis(azanetriyl))tetraacetic acid Fluorescein, 2′,7′-bis[[bis(carboxymethyl)amino]methyl]- Spiro[isobenzofuran-1(3h),9′-[9h]xanthene], glycine deriv. 2,7-bis[n,n-bis(carboxymethyl)aminomethylene]- Uo-rescein Acetic acid, [(3′,6′-dihydroxy-2′,7′- Uorandiyl)bis-(methylenenitrilo)]tetra- Nsc 298193 Spiro[isobenzofuran-1(3h),9′-[9h]xanthene], glycine derivative Fluorescein, 2 0 ,7 0 -bis[[bis(carboxymethyl) amino]methyl]- Spiro[isobenzofuran-1(3 h ),9 0 -[9 h ]xanthene], Glycine derivative Acetic Acid, [(3 0 ,6 0 -dihydroxy-2 0 ,7 0 -fluor- andiyl)bis(methylenenitrilo)]tetra- Calcein indicator for metal determination Calcein, Suitable for fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium. Calcein, AR CALCEINCALCEINCALCEIN 3,3'-Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein (Calcein) Calcein, GR