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PNGASE F

PNGASE F structure

CAS No.: 83534-39-8

Chemical Name:: PNGASE F

Synonyms: PNGASE A;PNGASE F;EC 3.5.1.52;N-GLYCANASE;GLYCOPEPTIDASE;N-GLYCANASE(R);PNGase F（Fast）;N-GLYCOSIDASE A;N-GLYCOSIDASE F;GLYCOPEPTIDASE A

CBNumber:: CB2114680

Molecular Formula:

Molecular Weight:: 0

MDL Number:: MFCD00131222

MOL File:: Mol file

MSDS File:: SDS

Last updated:2023-04-23 13:52:06

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PNGASE F Properties

storage temp.	2-8°C
form	buffered aqueous solution

SAFETY

Risk and Safety Statements

Symbol(GHS)	GHS08
Signal word	Danger
Hazard statements	H334
Precautionary statements	P261-P284-P501
Hazard Codes	Xi,N,Xn
Risk Statements	37/38-41-50/53-43-20/21/22
Safety Statements	22-24/25-36/37/39-26-61
RIDADR	UN 3316 9
WGK Germany	2

PNGASE F price More Price(16)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	P7367	PNGase F from Elizabethkingia meningoseptica BioReagent, ≥95% (SDS-PAGE), for proteomics	83534-39-8	50units	$407	2024-03-01	Buy
Sigma-Aldrich	P7367	PNGase F from Elizabethkingia meningoseptica BioReagent, ≥95% (SDS-PAGE), for proteomics	83534-39-8	300Units	$1800	2024-03-01	Buy
Sigma-Aldrich	G5166	PNGase F from Elizabethkingia miricola buffered aqueous solution	83534-39-8	50units	$330	2024-03-01	Buy
Sigma-Aldrich	G5166	PNGase F from Elizabethkingia miricola buffered aqueous solution	83534-39-8	100units	$540	2024-03-01	Buy
Sigma-Aldrich	G1549	PNGase F from Elizabethkingia meningoseptica buffered aqueous solution, recombinant, expressed in E. coli	83534-39-8	50units	$272	2024-03-01	Buy

Product number	Packaging	Price	Buy
P7367	50units	$407	Buy
P7367	300Units	$1800	Buy
G5166	50units	$330	Buy
G5166	100units	$540	Buy
G1549	50units	$272	Buy

PNGASE F Chemical Properties,Uses,Production

Uses

Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp.

Uses

PNGase F from Elizabethkingia meningoseptica has been used:

for de-N-glycosylation of Zika20virus E protein
to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells
to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)

Uses

Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan.

General Description

PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

PNGASE F Preparation Products And Raw materials

Raw materials

Preparation Products

PNGASE F Suppliers

Global( 58)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
GlycoPharm.LLC	+86-27-63498076	sale@glycolipidpharm.com	CHINA	158	58
Creative Enzymes	1-516-855-7709	info@creative-enzymes.com	United States	8748	58
Career Henan Chemica Co	+86-0371-86658258 15093356674;	laboratory@coreychem.com	China	30255	58
Shanghai Acmec Biochemical Technology Co., Ltd.	+undefined18621343501	product@acmec-e.com	China	33349	58
Aladdin Scientific	+1-833-552-7181	sales@aladdinsci.com	United States	52927	58
Wuhan Chemstan Biotechnology Co., Ltd.	15926423062	422450190@qq.com	China	10132	58
Wuhan Tangzhi Pharmaceutical Co., Ltd.	027-63498076 13658392159	sale@glycogene.com	China	480	58
3B Pharmachem (Wuhan) International Co.,Ltd.	821-50328103-801 18930552037	3bsc@sina.com	China	15848	69
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80
Anhui kuer Bioengineering Co., Ltd	0551-65171243 13810511747	kuer@kuerhuaxue.com	China	2997	55

Supplier	Advantage
GlycoPharm.LLC	58
Creative Enzymes	58
Career Henan Chemica Co	58
Shanghai Acmec Biochemical Technology Co., Ltd.	58
Aladdin Scientific	58
Wuhan Chemstan Biotechnology Co., Ltd.	58
Wuhan Tangzhi Pharmaceutical Co., Ltd.	58
3B Pharmachem (Wuhan) International Co.,Ltd.	69
Sigma-Aldrich	80
Anhui kuer Bioengineering Co., Ltd	55

83534-39-8(PNGASE F)Related Search:

METHYL ISOCYANOACETATE Ethyl isocyanoacetate DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) COBALT(II) ACETYLACETONATE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III)

Benzyl isocyanide TERT-BUTYL ISOCYANIDE Cupric acetylacetonate 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE Aluminum acetylacetonate

Tris(2,4-pentanedionato)chroMiuM(III) Ferric acetylacetonate PHENYLSELENOL TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) Tosylmethyl isocyanide

SALCOMINE N-BUTYLISOCYANIDE Gentamicin sulfate

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PEPTIDE-N4-(ACETYL-BETA-GLUCOSAMINYL)-ASPARAGINE AMIDASE (PEPTIDE-N4-[N-ACETYL-BETA-GLUCOSAMINYL)] PEPTIDE N-GLYCOSIDASE PEPTIDE-N-GLYCOSIDASE F PNGASE F HIGH CONCENTRATION FROM CHRYSEO PNGASE F RECOMBINANT CHRYSEOBACTERIUM GLYCOPEPTIDASE FROM ALMONDS glycopeptidase a from almonds pngase f from elizabethkingia (chryseobacterium/flavobacterium) meningsepticum GLYCOPEPTIDASE F, RECOMBINANT, SOLUTION, FROM FLAVOBACTERIUM MENINGOSEPTICUM N-Glycanase, N-GlycosidaseF, Peptide N-glycosidase, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum N-Glycosidase A, PNGase A, N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase N-GlycosidaseF, Peptide N-glycosidase, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum PNGaseF from Elizabethkingia meningoseptica PNGASE FROM ELIZABETHKINGIA (CHRYSEOBACTERIUM/FLAVOBACTERIUM) MENINGSEPTICUM, RECOMBINANT EXPRESSED IN ESCHERICHIA CO PNGASE FROM ELIZABETHKINGIA (CHRYSEOBACTERIUM/FLAVOBACTERIUM) MENINGSEPTICUM, PROTEOMICS GRADE peptide-N4-(N-acetyl--glucosaMinyl) asparagineaMidase Glycopeptidase F from Elizabethkingia meningoseptica PNGase F from Elizabethkingia miricola N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase EC 3.5.1.52 GLYCOPEPTIDASE GLYCOPEPTIDASE A GLYCOPEPTIDASE F N-LINKED-GLYCOPEPTIDE-[N-ACETYL-BETA-D-GLUCOSAMINYL]-L-ASPARAGINE AMIDOHYDROLASE N-GLYCANASE N-GLYCANASE(R) N-GLYCOSIDASE A N-GLYCOSIDASE A, ALMOND N-GLYCOSIDASE F N-GLYCOSIDASE F, CHRYSEOBACTERIUM MENINGOSEPTICUM PNGASE A PNGASE F PNGASE (F CHRYSEOBACTERIUM (FLAVOBACTERIUM) MENINGOSEPTICUM) peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase PNGase F from Elizabethkingia meningoseptica, Recombinant Native Flavobacterium meningosepticum PNGase F Native Elizabethkingia miricola PNGase F PNGase F from Flavobacterium meningosepticum, Recombinant PNGase F from Elizabethkingia miricola, Recombinant Native Elizabethkingia meningoseptica PNGase F Native Almonds Glycopeptidase A N-Glycosidase F, Elizabethkingia meningosepticum N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli buffered aqueous solution, recombinant, expressed in E. coli PNGase F from Elizabethkingia meningoseptica lyophilized powder, recombinant, expressed in E. coli Glycopeptidase A from almonds buffered aqueous glycerol solution, >=0.05 unit/mL PNGase F from Elizabethkingia miricola buffered aqueous solution PNGase F from Elizabethkingia meningoseptica BioReagent, >=95% (SDS-PAGE), for proteomics PNGase F from Elizabethkingia meningoseptica buffered aqueous solution, recombinant, expressed in E. coli PNGase F（Fast） 83534-39-8 Post-translational Modification Proteomics and Protein Expression Proteomics Enzymes for Protein Carbohydrate Modification Analysis Endoglycosidases Enzymes, Inhibitors, and Substrates