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PBR322 PLASMID DNA

CAS No.
93384-17-9
Chemical Name:
PBR322 PLASMID DNA
Synonyms
PBR322 DNA;DNA, PBR322;PBR322 VECTOR;PBR322 PLASMID DNA;PBR325 PLASMID DNA;PLASMID PBR322 DNA;PLASMID PBR322 DEOXYRIBONUCLEIC ACID;Deoxyribonucleic acids, plasmid pBR322;pBR322 Plasmid DNA from E. coli RRI;DEOXYRIBONUCLEIC ACID, PLASMID (PBR 322 PLASMID)
CBNumber:
CB3353064
Molecular Formula:
Molecular Weight:
0
MDL Number:
MFCD00146650
MOL File:
Mol file
Last updated:2023-04-23 13:52:06

PBR322 PLASMID DNA Properties

storage temp. -20°C
form lyophilized powder

SAFETY

Risk and Safety Statements

WGK Germany  3
3-10-21

PBR322 PLASMID DNA price More Price(5)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich D9893 pBR322 Plasmid DNA from E. coli RRI lyophilized powder 93384-17-9 1unit $186.8 2024-03-01 Buy
Sigma-Aldrich D4904 pBR322 Plasmid DNA from E. coli RRI buffered aqueous solution 93384-17-9 0.25unit $156 2024-03-01 Buy
Sigma-Aldrich D9893 pBR322 Plasmid DNA from E. coli RRI lyophilized powder 93384-17-9 5units $1870 2024-03-01 Buy
Sigma-Aldrich D4904 pBR322 Plasmid DNA from E. coli RRI buffered aqueous solution 93384-17-9 1unit $498 2024-03-01 Buy
Sigma-Aldrich D4904 pBR322 Plasmid DNA from E. coli RRI buffered aqueous solution 93384-17-9 5units $1740 2024-03-01 Buy
Product number Packaging Price Buy
D9893 1unit $186.8 Buy
D4904 0.25unit $156 Buy
D9893 5units $1870 Buy
D4904 1unit $498 Buy
D4904 5units $1740 Buy

PBR322 PLASMID DNA Chemical Properties,Uses,Production

Uses

Select for recombinant DNA. The Pst I and Pvu I cleavage sites in the ampicillin gene, or the Cla I, Hind III, BamH I, and Sal I sites in the tetracycline gene allow the insertion of foreign DNA fragments, and inactivation of one of these genes. pBR322 DNA has been used in to study artificial metallonuclease activity.

Uses

pBR322 Plasmid DNA from?E. coli?RRI has been used to study about ferrous ion-induced strand breaks in the plasmid pBR322 are mediated through hydrogen peroxide.

Uses

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication.

General Description

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in E. coli. This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. Plasmid pBR322 confers resistance to ampicillin and tetracycline. The plasmid sequence has been published.

The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).

Biochem/physiol Actions

This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives.

PBR322 PLASMID DNA Preparation Products And Raw materials

Raw materials

Preparation Products

PBR322 PLASMID DNA Suppliers

Global( 42)Suppliers
Supplier Tel Email Country ProdList Advantage
Career Henan Chemica Co
+86-0371-86658258 15093356674; laboratory@coreychem.com China 30255 58
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51471 80
Shanghai Yingxin laboratory equipment Co., Ltd 021-021-59178156 15300768757 359382369@qq.com China 10367 58
Shanghai Guchen Biotechnology Co., LTD 021-34675735 19147740836 1986399151@qq.com China 9849 58
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