SUCCINYL COENZYME A SODIUM SALT

SUCCINYL COENZYME A SODIUM SALT 속성

SUCCINYL COENZYME A SODIUM SALT C화학적 특성, 용도, 생산

개요

Succinyl-coenzyme A (succinyl-CoA) is an intermediate in the citric acid cycle. It is converted to succinate, which is a metabolic intermediate with various biological activities. Succinyl-CoA is required, with glycine, to form δ-aminolevulinic acid in the first step of porphyrin and heme synthesis. Succinyl-CoA deficiency, caused by vitamin B₁₂ deficiency can disrupt heme and energy production, leading to neuromotor dysfunction.

용도

Succinyl coenzyme A sodium salt has been used:

• for the nonenzymatic succinylation of lysine in vitro,
• as a substrate for adipic acid biosynthesis in recombinant E. coli
• in isocitrate dehydrogenase (ICDH)?treatment for the succinylation of proteins
• as a substrate to study the specificity and kinetics of enzymes such as acetate:succinate CoA-transferase and 5-aminolevulinate synthase (ALA synthase)

Purification Methods

If it should be purified further, then it should be dissolved in H2O (0.05g/mL) adjusted to pH 1 with 2M H2SO4 and extracted several times with Et2O. Excess Et2O is removed from the aqueous layer by bubbling N2 through it and is stored frozen at pH 1. When required, the pH should be adjusted to 7 with dilute NaOH and used within 2 weeks (samples should be frozen). Succinyl coenzyme A is estimated by the hydroxamic acid method [Hersch & Jencks J Biol Chem 242 3468 1967]. It is more stable in acidic than in neutral aqueous solutions, but neutral solutions can be stored at –15o with negligible decomposition. [Jordan & Laghai-Newton Methods Enzymol 123 435 1986, Beilstein 26 III/IV 3666.]

SUCCINYL COENZYME A SODIUM SALT 준비 용품 및 원자재

원자재

준비 용품

SUCCINYL COENZYME A SODIUM SALT 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Hefei TNJ Chemical Industry Co.,Ltd.	0551-65418684 +8618949823763	sales@tnjchem.com	China	25363	58
Aladdin Scientific	+1-833-552-7181	sales@aladdinsci.com	United States	57511	58
Shanghai Yingxin laboratory equipment Co., Ltd	021-021-59178156 15300768757	359382369@qq.com	China	10367	58
Chengdu HuaXia Chemical Reagent Co. Ltd	400-1166-196 13458535857	cdhxsj@163.com	China	13358	58
Shanghai Macklin Biochemical Co.,Ltd.	15221275939 15221275939	shenlinxing@macklin.cn	China	15878	55
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80
Shanghai EFE Biological Technology Co., Ltd.	021-65675885 18964387627	info@efebio.com	China	9709	58
Shanghai Hongye Biotechnology Co. Ltd	400-9205774	sales@glpbio.cn	China	6870	58
ChemeGen(Shanghai) Biotechnology Co.,Ltd.	18818260767	sales@chemegen.com	China	11289	58
Hubei Xinyang Medical Technology Co., Ltd	15347293736 15347293736	2853117764@qq.com	China	8854	58

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