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Sigma-Aldrich |
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021-61415566 800-8193336 |
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| | EC 1.13.11.3 Basic information |
| Product Name: | EC 1.13.11.3 | | Synonyms: | PROTOCATECHUATE 3,4-DIOXYGENASE;protocatechuate 3,4-dioxygenase from*pseudomonas;PROTOCATECHUATE 3,4-DIOXYGENASE FROM*PSE UDOMONAS SP;frompseudomonasspecies,lyophilizedpowderactivity:3-5umgsolidcontainsapprox.40;EC 1.13.11.3;Protocatechuate-3,4-dioxygenase,from Pserdomonas sp.;Protocatechuate 3,4-Dioxygenase, recombinant from bacteria, solution;rPCO, Protocatechuate: oxygen 3,4-oxidoreductase (decyclizing) | | CAS: | 9029-47-4 | | MF: | | | MW: | 0 | | EINECS: | | | Product Categories: | 1.13.x.x Acting on single donors with incorporation of molecular oxygen;1.x.x.x Oxidoreductases;Application Index;Diagnostic and Analytical EnzymesMetabolomics;Enzymes, Inhibitors, and Substrates;General Metabolic Enzymes;General Metabolic Enzymes I-ZEnzyme Class Index;enzyme | | Mol File: | Mol File | ![EC 1.13.11.3 Structure]() |
| | EC 1.13.11.3 Chemical Properties |
| storage temp. | -20°C | | form | lyophilized powder | | color | White to off-white | | biological source | bacterial (Pseudomonas spp.) | | Specific Activity | ≥3units/mg solid |
| WGK Germany | 3 | | Storage Class | 11 - Combustible Solids |
| | EC 1.13.11.3 Usage And Synthesis |
| Uses | The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into β-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay. | | General Description | Protocatechuate 3,4-Dioxygenase belongs to the non-heme iron family of enzymes. The active site of the enzyme contains Fe3+. | | Biochem/physiol Actions | Protocatechuate 3,4-Dioxygenase catalyzes the degradation of 3,4-dihydroxybenzoate (protocatechuate) into β-carboxy-cis,cis-muconate. |
| | EC 1.13.11.3 Preparation Products And Raw materials |
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