4-Methyl-2-oxovaleric acid synthesis
- Product Name:4-Methyl-2-oxovaleric acid
- CAS Number:816-66-0
- Molecular formula:C6H10O3
- Molecular Weight:130.14
61-90-5
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$10.00/25G
127-17-3
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$5.00/10g
816-66-0
155 suppliers
$14.00/250mg
56-41-7
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$5.00/5g
Yield:-
Reaction Conditions:
with pyridoxal 5'-phosphate;recombinant ω-transaminase from Burkholderia vietnamiensis G4 in aq. phosphate buffer; pH=7.4 at 37; for 0.0833333 h;Enzymatic reaction;enantioselective reaction;
Steps:
2.7. Substrate specificity and enantioselectivity
General procedure: Amino donor specificity was assayed by following the similar procedure of Section 2.5. The amino donor substrates (10 mM) listed in Table 1 reacted with pyruvate (10 mM) as amino acceptor. For racemic amino donor, the concentration was 20 mM. After the reaction, residual pyruvate was analyzed by HPLC according to Section 2.10. Amino acceptor specificity was assayed by following the similar procedure of Section 2.5. The amino acceptor substrates (10 mM) listed in Table 2 reacted with (S)-α-MBA (10 mM) as amino donor. After the reaction, the amount of produced acetophenone was analyzed and the ee values of the produced amines were determined according to Section 2.10.
References:
Jiang, Jinju;Chen, Xi;Feng, Jinhui;Wu, Qiaqing;Zhu, Dunming [Journal of Molecular Catalysis B: Enzymatic,2014,vol. 100,p. 32 - 39]
61-90-5
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$10.00/25G
816-66-0
155 suppliers
$14.00/250mg
20312-37-2
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$23.00/250mg
61-90-5
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$10.00/25G
816-66-0
155 suppliers
$14.00/250mg
13748-90-8
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$14.00/1g
4502-00-5
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$29.00/1g
816-66-0
155 suppliers
$14.00/250mg
26073-09-6
45 suppliers
$220.00/250mg
816-66-0
155 suppliers
$14.00/250mg