Back to ChemicalBook Home--->CAS DataBase List--->5550-12-9

5550-12-9

5550-12-9 Structure

5550-12-9 Structure
IdentificationMore
[Name]

Guanosine 5'-monophosphate disodium salt
[CAS]

5550-12-9
[Synonyms]

5'-GMP 2NA
5'-GMP-NA2
5'-guanylic acid disodium salt
DISODIUM 5'-GUANYLATE
DISODIUM GUANOSINE-5'-MONOPHOSPHATE
FEMA 3668
G-5'-P DISODIUM SALT
GMP
GMP DISODIUM SALT
GMP: G-5'-P DISODIUM SALT
guanosine 5'-(disodium phosphate)
GUANOSINE 5'-MONOPHOSPHATE DISODIUM SALT
GUANOSINE 5'-MONOPHOSPHATE DISODIUM SALT N-HYDRATE
GUANOSINE-5'-MONOPHOSPHORIC ACID, DISODIUM
GUANOSINE 5'-MONOPHOSPHORIC ACID DISODIUM SALT
GUANOSINE-5'-PHOSPHATE DISODIUM SALT
GUANYLIC ACID SODIUM SALT
SODIUM GUANYLATE
5’-gmpdisodiumsalt
disodium5’-gmp
[EINECS(EC#)]

226-914-1
[Molecular Formula]

C10H12N5Na2O8P
[MDL Number]

MFCD00070575
[Molecular Weight]

407.18
[MOL File]

5550-12-9.mol
Chemical PropertiesBack Directory
[Appearance]

White crystalline powder
[mp ]

300°C
[FEMA ]

3668
[storage temp. ]

2-8°C
[Water Solubility ]

>50 g/L
[Detection Methods]

HPLC,NMR
[CAS DataBase Reference]

5550-12-9(CAS DataBase Reference)
[EPA Substance Registry System]

5550-12-9(EPA Substance)
Safety DataBack Directory
[Hazard Codes ]

Xi
[Risk Statements ]

R36/37/38:Irritating to eyes, respiratory system and skin .
[Safety Statements ]

S22:Do not breathe dust .
S24/25:Avoid contact with skin and eyes .
S36/37/39:Wear suitable protective clothing, gloves and eye/face protection .
S27:Take off immediately all contaminated clothing .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
[WGK Germany ]

2
[RTECS ]

MF9290000
[HS Code ]

29339900
Raw materials And Preparation ProductsBack Directory
[Raw materials]

Carbon disulphide-->D(+)-Glucose-->Ribonucleic acid-->5'-Guanylic acid-->2-Amino-9-beta-D-ribofuranosyl-9H-purine-6-(1H)-one hydrate
[Preparation Products]

Disodium 5'-Inosinate-->Inosine-->CALCIUM5'-RIBONUCLEOTIDES-->Calcium 5'-ribonucleotide
Questions And AnswerBack Directory
[Identification test]

UV absorbance: A 1/50000 sample solution was prepared with 0.01 mol/L hydrochloric acid solution, which had a maximum absorbance at a wavelength of 256 nm ± 2 nm. A250/A260 ratio is at the range of 0.95 to 1.03, and A280/A260 ratio is at the range of 0.63 to 0.71.
Ribose test exhibits positive result. Take 3 mL of 0.03% sample solution, add 0.2 mL of the ethanol solution of 10% dihydroxy toluene, and 3 mL 0.1% ammonium ferric sulfate hydrochloric acid (TS-100), heat in water bath for 10 min, the color of the solution should be green.
Organic phosphate test is positive. Take 5 mL of 1% sample solution plus 2 mL of magnesium oxide mixture solution (TS-133), there should be no precipitation occurred. Further add 7 mL of nitric acid and boil for 10min, neutralize with sodium hydroxide solution (TS-224) and should exhibit phosphate reaction (IT-26).
The sodium salt test was positive (IT-28).
Solubility: it is soluble in water, slightly soluble in ethanol but almost insoluble in acetone and ether (OT-42).
[Content analysis]

Accurately weigh 500 mg of sample and dissolve with 0.01 mol/L hydrochloric acid, and add the volume up to 1000 ml. Take 10 mL of this solution, and then add 0.01mol/L hydrochloric acid to a final volume of 250 ml and mix. Use 0.01mol/L hydrochloric acid as a control, place it in 1 cm cuvette and measure the absorbance A under the wavelength of 260nm and then calculated as follows:
Amount (%) = A/289.8 × 250,000/Sample amount (mg) × 100/(100-Moisture (%)) × 100
[Toxicity]

ADI is not subject to special provision (FAO/WHO, 2001).
LD50:10 mg/kg (rat, oral). After chronic test feeding of 0.1% to 1% feed for 6 months, weight and tissue get no abnormal changes.
It can be safely used in food products (FDA, §172.530, 2000).
EEC-HACSG provides that it should not be used for infant food.
[Usage limit]

GB 2760-96: all kinds of food, take GMP limit.
FAO WHO: luncheon meat, ham, bacon, etc., 500 mg kg (in guanosine), broth and soup, GMP.
Standard for Maximum Allowable amount and maximal allowable residue of Food Additives
Name of additive    Food allowed to use it as additive    Function of additive    Maximal allowable amount(g/kg)    Maximal allowable amount(g/kg)
5'-guanylate disodium    Food    Food purpose flavor    The perfume ingredients used in formulating fragrances shall not exceed the maximal allowable amount documented in GB 2760   
5'-guanylate disodium    Food    Flavoring agent    Apply appropriate amount based on the demand of production except in cases where there is specific rule
[Chemical properties]

It appears as colorless to white crystalline or crystalline powder, usually containing 7 molecules of crystal water, being odorless, having unique flavor similar to mushrooms. Melting point is not obvious. It undergoes browning under 240 ℃ and decomposition under 250 ℃. It is stable against acid, alkali, salt and heat. It has strong moisture absorption property and is easily soluble in water (25 ℃, 25%). The pH value of its 5% aqueous solution is 7.0 to 8.5, being slightly soluble in ethanol, acetone and ether. Mouse: oral LD50: 10g/kg, ADI does not require special rule(FAO/WHO, 1994).
[Uses]

Guanylate sodium is allowed flavoring agent allowed at home and abroad, often used together with MSG and sodium inosinate. When mixed for usage, it has multiplication effect on the taste. It can be used for all types of food and should be subject to appropriate use according to production needs.
It is mainly used as condiments, being a new generation food by freshener.
It is a kind of flavor agent with mushrooms flavor. It is often used in conjunction with sodium glutamate (adding amount is 1%~5%), there is a very significant increase in freshener effect.
[Preparation]

The production methods of guanylic acid mainly include enzymatic hydrolysis and fermentation method. In the fermentation method, there are two-step method and combination method with biosynthesis and chemical synthesis, two kinds which are of industrial significance.
(1) Ribonucleic acid (RNA) hydrolysis method. See the production method of 5'-inosinic acid disodium.
(2) Two-step method. Take glucose as the carbon source and ferment with the Bacillus subtilis mutant to obtain the guanosine. The production level was 10.5 g/L. Then guanosine, in pyridine solution, is acidified with phosphorus oxychloride phosphate to obtain guanylate.
(3) Biosynthesis and chemical synthesis.
At pH=7, apply Bacillus megaterium (No. 336) for fermentation of glucose (8%) for 90 h at pH = 7, producing 15 g/L 5-amino-4-carboxamide nucleoside (AICAr); then extract by ion exchange, followed by concentration and drying to be dissolved in methanol containing NaOH. Add carbon disulfide for co-heating so that it is converted into 2-thioglycoside; It is finally oxidized with hydrogen peroxide; add excess ammonia and heat to obtain guanosine which is further converted to guanylate through phosphorylation.
Well-known Reagent Company Product InformationBack Directory
[Acros Organics]

Guanosine 5'-monophosphate, disodium salt hydrate, 97%(5550-12-9)
[Alfa Aesar]

Guanosine 5'-monophosphate disodium salt heptahydrate(5550-12-9)
[Sigma Aldrich]

5550-12-9(sigmaaldrich)
[TCI AMERICA]

Guanylic Acid Sodium Salt  (2'-,3'-mixture) from Yeast(5550-12-9)
5550-12-9 suppliers list
Company Name: Henan DaKen Chemical CO.,LTD.
Tel: +86-371-55531817
Fax:
Website: www.dakentech.com
Company Name: Shanghai Zheyan Biotech Co., Ltd.
Tel: 18017610038
Fax:
Website: www.chemicalbook.com/ShowSupplierProductsList30845/0.htm
Company Name: career henan chemical co
Tel: +86-371-86658258
Fax:
Website: www.coreychem.com
Company Name: Mainchem Co., Ltd.
Tel: +86-0592-6210733
Fax: +86-0592-6210733
Website: www.mainchem.com
Company Name: Henan Tianfu Chemical Co.,Ltd.
Tel: 0371-55170693
Fax: 0371-55170693
Website: www.tianfuchem.com
Company Name: J & K SCIENTIFIC LTD.  
Tel:400-666-7788 +86-10-82848833
Fax:+86-10-82849933
Website:www.jkchemical.com
Company Name: Meryer (Shanghai) Chemical Technology Co., Ltd.  
Tel:+86-(0)21-61259100(Shanghai) +86-(0)755-86170099(ShenZhen) +86-(0)10-62670440(Beijing)
Fax:+86-(0)21-61259102(Shanghai) +86-(0)755-86170066(ShenZhen) +86-(0)10-88580358(Beijing)
Website:www.meryer.com
Company Name: 3B Pharmachem (Wuhan) International Co.,Ltd.  
Tel:86-21-50328103 * 801、802、803、804 Mobile:18930552037
Fax:86-21-50328109
Website:
Company Name: Hongene Biotechnology Limited  
Tel:86-21-64757213
Fax:86-21-64700613
Website:www.hongene.com
Company Name: TAIYUAN RHF CO.,LTD.  
Tel:+86 351 7031519
Fax:+86 351 7031519
Website:www.rhfchem.com
Company Name: TCI (Shanghai) Development Co., Ltd.  
Tel:021-67121386 / 800-988-0390
Fax:021-67121385
Website:www.tcichemicals.com
Company Name: Energy Chemical  
Tel:021-58432009 / 400-005-6266
Fax:021-58436166-800
Website:www.energy-chemical.com
Company Name: BePharm Ltd  
Tel:4001-647-117; 021-61629020
Fax:021-61629029
Website:www.bepharm.com
Company Name: JinYan Chemicals(ShangHai) Co.,Ltd.  
Tel:13817811078,021-50426030
Fax:86-021-50426522,50426273
Website:www.jingyan-chemical.com
Company Name: Jia Xing Isenchem Co.,Ltd  
Tel:0573-85280080,18627885956
Fax:0573-85285100
Website:www.isenchem.com
Company Name: Adamas Reagent, Ltd.  
Tel:400-600-9262
Fax:
Website:www.tansoole.com
Company Name: Chemfun Medical Technology(Shanghai) Co., Ltd.  
Tel:021-67220633 & 021-37212706
Fax:+86-21-67220638
Website:www.chemfun.net
Company Name: Nanjing Chemlin Chemical Co., Ltd  
Tel:025-83697070
Fax:+86-25-83453306
Website:www.echemlin.cn
Tags:5550-12-9 Related Product Information
7440-23-5 73-40-5 58-63-9 4691-65-0 57248-88-1 7601-54-9 139-33-3 22560-50-5 10163-15-2 59277-89-3 55896-93-0 85-32-5 5550-12-9 7647-14-5 1405-53-4 10049-21-5 68-04-2 126-73-8