| Identification | More | [Name]
BIOTIN HYDRAZIDE | [CAS]
66640-86-6 | [Synonyms]
(+)-BIOTIN HYDRAZIDE
BIOTIN HYDRAZIDE
BIOTIN (LONG ARM) HYDRAZIDE
1H-Thieno(3,4-d)imidazole-4-pentanoic acid, hexahydro-2-oxo-, hydrazid e, (3aS,4S,6aR)-
Biotin-NHNH2 | [EINECS(EC#)]
613-970-0 | [Molecular Formula]
C10H18N4O2S | [MDL Number]
MFCD00078532 | [Molecular Weight]
258.34 | [MOL File]
66640-86-6.mol |
| Chemical Properties | Back Directory | [Melting point ]
245-247 °C
| [Boiling point ]
633.1±40.0 °C(Predicted) | [density ]
1.243±0.06 g/cm3(Predicted) | [storage temp. ]
2-8°C
| [solubility ]
DMSO: ≤20 mg/mL
| [form ]
powder
| [pka]
13.25±0.35(Predicted) | [color ]
White to Almost white | [BRN ]
28347 | [InChI]
InChI=1S/C10H18N4O2S/c11-14-8(15)4-2-1-3-7-9-6(5-17-7)12-10(16)13-9/h6-7,9H,1-5,11H2,(H,14,15)(H2,12,13,16)/t6-,7-,9-/m0/s1 | [InChIKey]
KOZWHQPRAOJMBN-ZKWXMUAHSA-N | [SMILES]
C1(=O)N[C@]2([H])[C@H](CCCCC(NN)=O)SC[C@]2([H])N1 | [CAS DataBase Reference]
66640-86-6(CAS DataBase Reference) |
| Hazard Information | Back Directory | [Description]
Biotin hydrazide is a biotinyl derivative used to label surface functional groups, antibodies, lectins, sugars, nucleic acids, or molecules with free carboxylic or keto groups.1 Biotin hydrazide is used as a probe for determining protein carbonylation, an irreversible posttranslational modification resulting from the actions of reactive oxygen species or lipid oxidation products.2 The reaction of this probe is direct and does not require catalysts or reducing agents. | [Chemical Properties]
3.6 ? | [Uses]
(+)-Biotin hydrazide has been used:
- the modification of alginate
- for the labelling of mitochondrial proteins from non-muscle tissues
- as a component of glycoprotein staining solution
- in periodic acid-biotin-hydrazide (PABH) assay for mucins
- for labeling surface functional groups, biologically active molecules such as antibodies, lectins, sugars, nucleic acids or molecules with free carboxylic or keto groups.
- for coupling to glycoproteins through the carbohydrate by hydrazone formation
| [Uses]
aldehyde reactive biotinylation reagent | [Uses]
Biotin Hydrazide is a carbohydrate reactive biotinylation reagent used predominately for labeling monoclonal antibodies, unpaired cytosine residues in nucleic acids and glycoproteins. | [General Description]
Biotin hydrazide is a biotinylation reagent used to biotinylate glycoproteins with their sugar moieties. Biotin hydrazide can be used to prepare biotin-conjugated alginate. It can also be used for covalent attachment to PAAc via carbodi-imide cross linking. | [Purification Methods]
Wash the hydrazide with H2O, dry it, wash it with MeOH then Et2O, and dry. Recrystallise it from hot H2O (clusters of prisms) [Hofmann et al. J Biol Chem 144 513 1942]. [Beilstein 27 III/IV 7980.] | [References]
[1] E. DIAMANDIS T K C. The biotin-(strept)avidin system: principles and applications in biotechnology.[J]. Clinical chemistry, 1991, 37 5 1: 625-636. DOI: 10.1093/clinchem/37.5.625
[2] ASHRAF G. MADIAN Fred E R. Proteomic Identification of Carbonylated Proteins and Their Oxidation Sites[J]. Journal of Proteome Research, 2010, 9 8: 3766-3780. DOI: 10.1021/pr1002609 |
|
| Company Name: |
J & K SCIENTIFIC LTD.
|
| Telephone: |
18210857532 18210857532 |
| Website: |
https://www.jkchemical.com |
| Company Name: |
ChemShuttle, Inc.
|
| Telephone: |
0510-83588313-811 18800520310 |
| Website: |
www.jiehuapharma.com/ |
| Company Name: |
T&W GROUP
|
| Telephone: |
021-61551611 13296011611 |
| Website: |
www.trustwe.com/ |
|