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Oxidase, Cholesterin- Produkt Beschreibung

Cholesterol oxidase Struktur
9028-76-6
CAS-Nr.
9028-76-6
Bezeichnung:
Oxidase, Cholesterin-
Englisch Name:
Cholesterol oxidase
Synonyma:
REC-COD;C:C&B11;9028-76-6;EC 1.1.3.6;EC: 1.1.3.6;Choleterol oxidase;CHOLESTEROL OXIDASE;Cholesterin oxidase;Oxidase, cholesterol;Cholesterol oxidase20.4
CBNumber:
CB3152524
Summenformel:
NULL
Molgewicht:
0
MOL-Datei:
Mol file

Oxidase, Cholesterin- Eigenschaften

storage temp. 
-70°C
Löslichkeit
50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)
Aggregatzustand
solution (slightly hazy)
Farbe
pale yellow
EPA chemische Informationen
Oxidase, cholesterol (9028-76-6)

Sicherheit

S-Sätze: 24/25
WGK Germany  3
10-21
HS Code  35079090

Oxidase, Cholesterin- Chemische Eigenschaften,Einsatz,Produktion Methoden

Chemische Eigenschaften

Cholesterol oxidase is a bacterial-specific flavoenzyme that catalyzes the oxidation and isomerisation of steroids containing a 3β hydroxyl group and a double bond at the 5–6 of the steroid ring system. The enzyme is a member of a large family of flavin-specific oxidoreductases and is found in two different forms: one where the flavin adenine dinucleotide (FAD) cofactor is covalently linked to the protein and one where the cofactor is non-covalently bound to the protein.
Cholesterol oxidases are secreted bacterial enzymes that catalyze the first step in the degradation of cholesterol. They are flavoenzymes containing the redox cofactor, flavin adenine dinucleotide (FAD). The enzyme catalyzes three chemical conversions. In the first step, called the reductive half-reaction, the 3β-hydroxyl group of the steroid ring system is oxidized to the corresponding ketone. Key to this conversion is the FAD cofactor, which becomes reduced in the process. In the second step the enzyme catalyzes isomerization of the double bond in the oxidized steroid ring system from the 5–6 position to 4–5 position, to give the final steroid product, cholest-4-en-3-one. In the final step of the enzyme, called the oxidative half-reaction, the reduced cofactor reacts with dioxygen and is thus reoxidized while O2 is reduced to H2O2.

Verwenden

Cholesterol oxidase has been used in a study to demonstrate that microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. Cholesterol oxidase has also been used in a study that concluded that amphipaths that activate cholesterol might be useful in treating NPC disease.

Verwenden

Cholesterol oxidases are part of a unique class of enzymes that are soluble proteins although they interact with highly insoluble substrates. The natural substrate, cholesterol, is an important membrane component exhibiting low solubility in the aqueous medium of the cell, hence the enzyme must interact with the lipid bilayer in order to for the substrate to partition out of the membrane and undergo oxidation at the enzyme active site. In this regard cholesterol oxidase is an interfacial enzyme as it binds transiently to the membrane surface during catalysis and can only access the substrate from the membrane phase. Other examples of interfacial enzymes include phospholipases (Berg et al., 2001; Gelb et al., 1995; Jain et al., 1995).
Cholesterol oxidase has a number of important commercial applications. Initial studies on the enzyme focused on its use for the determination of cholesterol in serum, HDL or LDL (see review, Smith and Brooks, 1976). Serum cholesterol levels are determined using a three-enzyme assay, including cholesterol esterase, cholesterol oxidase and peroxidase (Richmond, 1973; Allain et al., 1974; Richmond, 1976). More recently electrochemical biosensors with immobilized cholesterol oxidase have been employed to determine cholesterol levels in serum or food (Arya et al., 2007; Basu et al., 2007; Vidal et al., 2004). Determining the serum cholesterol levels is critical for the assessment of a variety of diseases including hypercholesterolemia, coronary heart disease and lipid disorders for estimating the risk of thrombosis and myocardial infarction.

Allgemeine Beschreibung

Cholesterol Oxidase produced from Streptomyces sp corresponds to a molecular weight of 62 kDa. It has a pH and temperature optimum of 7 and 37 °C, respectively. Cholesterol Oxidase comprises a FAD-binding and a steroid-binding domain.

Biochem/physiol Actions

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H2O2.

Oxidase, Cholesterin- Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


Oxidase, Cholesterin- Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global( 111)Lieferanten
Firmenname Telefon Fax E-Mail Land Produktkatalog Edge Rate
Shanghai Zheyan Biotech Co., Ltd.
18017610038
zheyansh@163.com CHINA 3623 58
career henan chemical co
+86-0371-55982848
sales@coreychem.com China 29954 58
Chongqing Chemdad Co., Ltd
+86-13650506873
sales@chemdad.com CHINA 37282 58
Antai Fine Chemical Technology Co.,Limited
18503026267
info@antaichem.com CHINA 9664 58
Creative Enzymes
1-631-619-7922
1-631-938-8127 info@creative-enzymes.com United States 8748 58
Hubei Xindesheng Material Technology Co., Ltd.
18971041571 +86-15071057538
vickyzhao@whdschem.com CHINA 154 58
HANGZHOU CLAP TECHNOLOGY CO.,LTD
13588875226 86-571-88216897、88216896
86-571-88216895 sales@hzclap.com CHINA 6469 58
ShenZhen Ipure Biology import and export company co.,ltd
18071025641 18071025641
eric@ipurechemical.com CHINA 11336 58
Dideu Industries Group Limited
029-88380327
Service@dideu.com CHINA 30014 58
AFINE CHEMICALS LIMITED
008657185134551
008657185134895 info@afinechem.com CHINA 15559 58

9028-76-6(Oxidase, Cholesterin-)Verwandte Suche:


  • EC 1.1.3.6
  • EC: 1.1.3.6
  • CHOLESTEROL OXIDASE
  • CHOLESTEROL OXIDASE MICROBIAL
  • CHOLESTEROL OXIDASE, NOCARDIA SPECIES
  • CHOLESTEROL: OXYGEN OXIDOREDUCTASE
  • CHOLESTEROL: OXYGEN OXIDOREDUCTASE MICROBIAL
  • cholesterol oxidase F. pseudomonas sp.
  • cholesterol oxidase from nocardia ery-thropolis
  • Cholesterol Oxidase from Pseudomonas sp.
  • Cholesterol Oxidase Nocardia erythropolis
  • Cholesterol Oxidase, Streptomyces sp.
  • Oxidase, cholesterol
  • CHOLESTEROL OXIDASE FROM PSEUDOMONASFLUO RESCENS
  • CHOLESTEROL OXIDASE FROMSTREPTOMYCES SPE CIES
  • CHOLESTEROL OXIDASE FROM NOCARDIA ERY-TH ROPOLIS, ~25 U/ML
  • CHOLESTEROL OXIDASE F. PSEUDOMONAS SP., ~5 U/MG
  • CHOLESTEROL OXIDASE FROM CELLULOMONASSPE CIES
  • Cholecalciferol(VitaminD)
  • CholesterolOxidaseExNocardiaSp.
  • fromnocardiaerythropolislyophilizedpowder
  • Cholesterol oxidase, recombinant E.coli min. 300 U/ml
  • Cholesterol oxidase, from E.coli, >=50 units/mg protein
  • CHOLESTEROL OXIDASE FROM BREVIBACTERIUM
  • 3-Hydroxysteroid oxidase
  • Cholesterin oxidase
  • Cholesterol oxidase, recombinant from Nocardia sp.
  • rCO, Cholesterol: oxygen oxidoreductase
  • Cholesterol oxidase recombinant, solution
  • Cholesterol Oxidase from microorganisms
  • Cholesterol Oxidase from Brevibacterium
  • Cholesteroloxidase/cholesterol oxidase/recoMbinantcholesteroloxidase /REC-COD
  • REC-COD
  • cholesterol oxidase from cellulomonas sp.
  • cholesterol oxidase from streptomyces sp.
  • CHOLESTEROL OXIDASE extrapure
  • Cholesterol Oxidase from Brevibacterium sp.
  • Cholesterol Oxidase microbial,Cholesterol: oxygen oxidoreductase
  • Cholesterol Oxidase from Pseudomonas fluorescens,Cholesterol: oxygen oxidoreductase
  • Cholesterol Oxidase from Streptomyces sp.,Cholesterol: oxygen oxidoreductase
  • Cholesterol Oxidase from Brevibacterium sp.,Cholesterol: oxygen oxidoreductase
  • Cholesterol Oxidase from Cellulomonas sp.,Cholesterol:oxygen oxidoreductase
  • Cholesterol Oxidase, Nocardia sp.
  • Cholesterol oxidase20.4
  • Choleterol oxidase
  • Native Streptomyces sp. Cholesterol Oxidase
  • Native Microorganism Cholesterol Oxidase
  • Cholesterol oxidase from E. coli, Recombinant (liquid)
  • Cholesterol oxidase from Nocardia sp., Recombinant
  • Cholesterol oxidase from E. coli, Recombinant
  • Native Nocardia erythropolis Cholesterol Oxidase
  • C:C&B11
  • Cholesterol oxidase USP/EP/BP
  • 9028-76-6
  • 9028-76-6
  • Enzyme Class Index
  • Enzymes, Inhibitors, and Substrates
  • Diagnostic and Analytical Enzymes
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