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Albumine, Butserum Produkt Beschreibung

Bovine albumin Struktur
9048-46-8
CAS-Nr.
9048-46-8
Bezeichnung:
Albumine, Butserum
Englisch Name:
Bovine albumin
Synonyma:
nhsa;BSAV;BSA-C;AC-BSA;abs9156;aminofusin;ALBUMIN, PIG;albumenpowder;SERUM ALBUMIN;ACETYLATED BSA
CBNumber:
CB5107573
Summenformel:
N/A
Molgewicht:
0
MOL-Datei:
Mol file

Albumine, Butserum Eigenschaften

RTECS-Nr.
MT6446000
storage temp. 
2-8°C
Löslichkeit
PBS: >40 mg/mL
Aggregatzustand
lyophilized powder
Farbe
clear clear, colorless
PH
6.5-7.5 (1% in 0.15 M sodium chloride)
Wasserlöslichkeit
Soluble in water.
Merck 
13,8542
Stabilität:
Stable. Incompatible with strong oxidizing agents and strong acids. Refrigerate.
EPA chemische Informationen
Albumins, blood serum (9048-46-8)
Sicherheit
  • Risiko- und Sicherheitserklärung
  • Gefahreninformationscode (GHS)
Kennzeichnung gefährlicher Xn
R-Sätze: 22-52/53-21/22
S-Sätze: 24/25-61-36/37
WGK Germany  3
10-21
TSCA  Yes
HS Code  35029000
Toxizität LD50 intravenous in monkey: > 12500mg/kg
Bildanzeige (GHS)
Alarmwort
Gefahrenhinweise
Code Gefahrenhinweise Gefahrenklasse Abteilung Alarmwort Symbol P-Code
H302 Gesundheitsschädlich bei Verschlucken. Akute Toxizität oral Kategorie 4 Warnung P264, P270, P301+P312, P330, P501
Sicherheit
P264 Nach Gebrauch gründlich waschen.
P264 Nach Gebrauch gründlich waschen.
P270 Bei Gebrauch nicht essen, trinken oder rauchen.
P330 Mund ausspülen.

Albumine, Butserum Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:

R22:Gesundheitsschädlich beim Verschlucken.

S-Sätze Betriebsanweisung:

S24/25:Berührung mit den Augen und der Haut vermeiden.

Beschreibung

Bovine serum albumin is a protein which has a key role in regulating blood colloidal osmotic pressure. A water-soluble protein composed of 583 amino acids, BSA has a calculated molecular weight of 66,430 Daltons. Six α-helices form three homologous domains of BSA. Depending on pH, BSA undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.
This BSA product is specifically evaluated and tested for the following properties:
Very low protease content / protease-free
Very low fatty acid content / fatty acid-free
Protease-free BSA is especially useful for applications like:
Enzyme assays
Protein-based assays
Protease-sensitive techniques that include:
a. Enzyme immunoassay (EIA)
b. Nucleic acid hybridization
c. Radioimmunoassay (RIA)

Chemische Eigenschaften

Crystalline powder

Verwenden

Suitable for cell culture media supplement.
Bovine serum albumin (BSA) Fraction V has uses in many applications such as stabilizing proteins in solution, blocking non-specific binding in immuno-assays, and as a protein standard in various protein assays.
Stabilizer for proteins and enzymes in solution
Blocking agent for immuno-assays (ie, western blot, IHC, IF)
Minimizes non-specific antibody binding
IgG- and protease-free
50 g of solid powder
Bovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody. It is also used as a blocking agent to reduce non-specific antibody binding in immuno-detection procedures such as western blotting, immunofluorescence, and IHC. It is also suitable for use in protease sensitive assays such as RIA and EIA, as well as nucleic acid hybridization. This BSA is IgG- and protease-free.

Application

Bovine Serum Albumin has been used:
as a constituent of medium used for the differentiation of human pluripotent stem cells
in enzyme-linked immunosorbent assay (ELISA) and sandwich ELISA
to permeabilize fetal cortical cells
This Sigma A7030 BSA has specifically been used as a component of blocking buffer in a peptide-based ELISA assay for analysis of COVID-19 / SARS-CoV-2 samples (Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020)).

Biologische Aktivität

Bovine Serum Albumin is a BSA (bovine serum albumin) is a widely used blocking reagent for use in immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISAs and Western blotting. Used to block non-specific binding of antibodies. Can also be used as a protein concentration standard in Bradford assay for protein quantification. BSA is also used in cell culture.?

läuterung methode

Albumin is purified by dissolving it in conductivity water and passage at 2-4o through two ion-exchange columns, each containing a 2:1 mixture of anionic and cationic resins (Amberlite IR-120, H-form, Amberlite IRA-400, OH -form). This treatment removes ions and lipid impurities. Care is taken to exclude CO2, and the solution is stored at -15o. [M.ller et al. Trans Faraday Soc 57 312 1961.] More complete lipid removal is achieved by lyophilising the de-ionised solution, covering the dried albumin (human serum) with a mixture of 5% glacial acetic acid (v/v) in iso-octane (previously dried with Na2SO4) and allowing it to stand at 0o (without agitation) for upwards of 6hours before decanting and discarding the extraction mixture, washing with iso-octane, re-extracting, and finally washing twice with iso-octane. The purified albumin is dried under vacuum for several hours, then dialyzed against water for 12-24hours at room temperature, lyophilised, and stored at -10oC [Goodman Science 125 1296 1957]. It has been recrystallised in high (35%) and in low (22%) EtOH solutions from Cohn's Fraction V. The high EtOH recrystallisation is as follows: To 1kg of Fraction V albumin paste at -5o is added 300mL of 0.4 M pH (pH 5.5) acetate buffer in 35% EtOH pre-cooled to -10o and 430 mL of 0.1 M NaOAc in 25% EtOH also at -10o. Best results are obtained by adding all of the buffer and about half of the NaOAc and stirring slowly for 1hour. The rest of the NaOAc is added when all the lumps have disintegrated. The mixture is set aside at -5o for several days to crystallise. 35% EtOH (1 L) is then added to dilute the crystalline suspension and lower the ionic strength prior to centrifugation at -5o (yield 80%). The crystals are further dissolved in 1.5 volumes of 15% EtOH/0.02M NaCl at -5o and clarified by filtration through washed, calcined diatomaceous earth. This solution may be recrystallised by re-adjusting to the conditions in the first crystallisation, or it may be recrystallised at 22% EtOH with the aid of a very small amount of decanol (enough to give a final concentration of 0.02%). Note that crystallisation from lower EtOH concentration gave better purification (i.e. by removing globulins and carbohydrates) and producing a more stable product. The low EtOH recrystallisation is as follows: To 1kg of Fraction V at -10o to -15o is added 500mL of 15% EtOH at -5o, stirred slowly until a uniform suspension is formed. To the 15% EtOH (500mL) is added sufficient 0.2M NaHCO3 solution (125-150mL) at 0o to bring the pH (1:10 dilution) to 5.3. Some temperature rise occurs, and care must be taken to keep the temperature < -5o. If the albumin is incompletely dissolved a small amount of H2O is added (100mL at a time at 0o, allowing 15minutes between additions). Undissolved albumin can be easily distinguished from small amounts of undissolved globulins, or as the last albumin dissolves, the appearance of the solution changes from milky white to hazy grey-green in colour. Keep the solution at -5o for 12hours and filter by suspending in 15g of washed fine calcined diatomaceous earth, and filtering using a Büchner funnel precoated with coarser diatomaceous earth. The filtrate may require two or more similar filtrations to give a clear solution. To crystallise the filtrate, add through a capillary pipette, and with careful stirring, 1/100volume of a solution containing10% decanol and 60% EtOH (at -10o), and seed with the needle-type albumin crystals. After 2-3days, crystallisation is complete. The crystals are centrifuged off. These are suspended with gentle mechanical stirring in one-third their weight of 0.005 M NaCl pre-cooled to 0o. With careful stirring, H2O (at 0o) is added slowly in an amount equal to 1.7 times the weight of the crystals. At this stage there is about 7% EtOH, and the temperature cannot be made lower than -2.5o to -1o. Clarify, and collect as above. [Cohn et al. J Am Chem Soc 69 1753 1947.] Human serum albumin has been purified simil

Albumine, Butserum Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


Albumine, Butserum Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global( 343)Lieferanten
Firmenname Telefon Fax E-Mail Land Produktkatalog Edge Rate
Shanghai Zheyan Biotech Co., Ltd.
18017610038
zheyansh@163.com CHINA 3623 58
SINOPRO.CO.LTD
0082-42-721-7177
0082-42-3676760 michael@sinopro.co.kr South Korea 300 58
Hebei Crovell Biotech Co Ltd
19930504644 whatsapp +8619930504644
86-0311-66562153 sales4@crovellbio.com CHINA 2886 58
Henan Tianfu Chemical Co.,Ltd.
0371-55170693
0371-55170693 info@tianfuchem.com China 22607 55
Hangzhou FandaChem Co.,Ltd.
008615858145714
+86-571-56059825 fandachem@gmail.com CHINA 8910 55
Shanxi Naipu Import and Export Co.,Ltd
+8613734021967
kaia@neputrading.com CHINA 1008 58
career henan chemical co
+86-371-86658258
sales@coreychem.com CHINA 29954 58
SHANDONG ZHI SHANG CHEMICAL CO.LTD
+86 18953170293
+86 0531-67809011 sales@sdzschem.com CHINA 2941 58
Hebei Guanlang Biotechnology Co., Ltd.
+8619930503282
sales3@crovellbio.com China 5930 58
Cangzhou Wanyou New Material Technology Co.,Ltd
18631714998
sales@czwytech.com CHINA 914 58

9048-46-8(Albumine, Butserum)Verwandte Suche:


  • BOVINE SERUM ALBUMIN, ACETYLATED
  • BSA ACETYLATED
  • BSA, METHYLATED
  • BSA-C
  • BSA-C ACETYLATED
  • METHYL ESTER OF BOVINE ALBUMIN
  • ACETYLATED BSA
  • AC-BSA
  • ALBUMIN, PIG
  • ALBUMIN ACETYLATED
  • ALBUMIN, BOVINE, ACETYLATED
  • ALBUMIN, BOVINE SERUM-C ACETYLATED
  • ALBUMIN, BOVINE, METHYLATED
  • ALBUMIN, BOVINE SERUM, METHYLATED
  • albumenpowder
  • Albumins,bloodserum
  • nhsa
  • OmniPur BSA, Fraction V, Cold Alcohol Isolation - CAS 9048-46-8 - Calbiochem
  • Albumins, serum
  • AMINO ACID COMPLEX
  • BSA LOW ENODOTOXIN
  • ALBUMIN, BOVINE (BSA), CRYSTALLINE
  • Albumin, Acetylated from bovine serum
  • ALBUMIN, BOVINE, FRACTION V COLD ALCOHOL ISOLATION
  • Albumin powder (egg)
  • compound amino acid
  • Albumin (calf serum acetylated)
  • albumin from guinea pig serum
  • albumin from sheep serum
  • albumin from bovine serum,protein standard
  • albumin from canine serum
  • albumin solution from bovine serum
  • albumin from donkey
  • albumin from dog serum
  • ALBUMINBOVINE,FRACTIONV,LYOPHILIZEDPOWDER
  • ALBUMINBOVINE,SERUM,FRACTIONV,HEAT-SHOCKISOLATION,BIOTECHGRADE
  • ALBUMIN BOVINE, ESSENTIALLY PROTEASE FREE, AUSTRALIAN ORIGIN
  • Albumin, from bovine serum, fraction V (pH 7.0)
  • Albumin fraction V (from bovine blood)
  • Albumin low endotoxin (from bovine blood)
  • Albumin crystallized (from bovine blood)
  • Albumin standard powder (heat shock isolation, from bovine blood)
  • Bovine albumin
  • Bovine serum albumin
  • Albumin,Bovine,Fract V
  • ALBUMIN BOVINE fraction V powder pH 5.0 to 5.2
  • ALBUMIN BOVINE fraction V powder pH 6.0 to 7.0
  • ALBUMIN BOVINE protease free fraction V powder pH 6.0 to 7.0
  • Albumin from bovine serum, protein standard, Protein standard
  • Rat albumin, RSA
  • Bovine albumin, BSA, Cohn FractionV
  • Albumin from bovine serum, protein standard, BSA solution, Protein standard (bovine serum albumin)
  • Albumin from rhesus monkey
  • Albumine bovine cryst.
  • Albumine bovine, fract. V, very low endotoxin
  • AlbuMin Bovine V
  • BSAV
  • AlbuMin, Bovine SeruM, Low Endotoxin, ChroMatoPur ChroMatographically Purified froM New Zealand
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