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5-Methylcytosine

5-Methylcytosine structure

CAS No.: 554-01-8

Chemical Name:: 5-Methylcytosine

Synonyms: 4-Amino-5-methylpyrimidin-2(1H)-one;5-methylcytosin;5-Methylcytosine;Cytosine, 5-methyl-;5-Methylcytosine,>96%;5-Methylcytosine (~90%);5-Methylcytosine, 97%, 97%;5-METHYLCYTOSINE USP/EP/BP;4-Amino-5-methyl-2-pyrimidinol;5-Methylcytosine, 10 mM in DMSO

CBNumber:: CB5154906

Molecular Formula:: C5H7N3O

Molecular Weight:: 125.13

MDL Number:: MFCD00233537

MOL File:: 554-01-8.mol

MSDS File:: SDS

TDS File:: TDS

Last updated:2026-09-12 18:53:14

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5-Methylcytosine Properties

Melting point	270°C (rough estimate)
Boiling point	232.57°C (rough estimate)
Density	1.2602 (rough estimate)
refractive index	1.6510 (estimate)
storage temp.	Keep in dark place,Inert atmosphere,Room temperature
solubility	Methanol (Sparingly, Heated)
pka	9.05±0.10(Predicted)
form	Crystalline
color	White to Off-White
Water Solubility	Soluble in water.
Merck	14,6050
InChI	InChI=1S/C5H7N3O/c1-3-2-7-5(9)8-4(3)6/h2H,1H3,(H3,6,7,8,9)
InChIKey	LRSASMSXMSNRBT-UHFFFAOYSA-N
SMILES	C1(=O)NC(N)=C(C)C=N1
CAS DataBase Reference	554-01-8(CAS DataBase Reference)
FDA UNII	6R795CQT4H

SAFETY

Risk and Safety Statements

Symbol(GHS)

GHS07

Signal word

Warning

Hazard statements

H319-H317

Precautionary statements

P261-P272-P280-P302+P352-P333+P313-P321-P363-P501-P264-P280-P305+P351+P338-P337+P313P

RTECS

UW7362350

NFPA 704

	1
0		0

5-Methylcytosine price More Price(56)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Cayman Chemical	38741	5-Methylcytosine ≥98%	554-01-8	1g	$39	2026-04-30	Buy
Cayman Chemical	38741	5-Methylcytosine ≥98%	554-01-8	5g	$174	2026-04-30	Buy
Cayman Chemical	38741	5-Methylcytosine ≥98%	554-01-8	10g	$308	2026-04-30	Buy
Usbiological	C9111	Cytosine, 5-Methyl PurifiedbyProteinGaffinitychromatography.		50ug	$403	2026-06-03	Buy
Usbiological	C9111	Cytosine, 5-Methyl PurifiedbyProteinGaffinitychromatography.		250ug	$672	2026-06-03	Buy

Product number	Packaging	Price	Buy
38741	1g	$39	Buy
38741	5g	$174	Buy
38741	10g	$308	Buy
C9111	50ug	$403	Buy
C9111	250ug	$672	Buy

5-Methylcytosine Chemical Properties, Uses, Production

Chemical Properties

Crystals in prisms from water.

Uses

5-Methylcytosine is used as a marker to protect DNA from being cut by native methylation-sensitive restriction enzymes. It is also employed in the production of 4-amino-5-hydroxy-5-methyl-2-oxo-2,5-dihydro-imidazole-1-carbaldehyde.

Definition

ChEBI: A pyrimidine that is a derivative of cytosine, having a methyl group at the 5-position.

IC 50

Microbial Metabolite; Human Endogenous Metabolite

Purification Methods

Crystallise it from water (solubility is 3.4%). The hydrochloride has m 299-301o (sintering at 280o) (from aqueous HCl/Me2CO). [Hitchings et al. J Biol Chem 177 537 1949, Cohn J Am Chem Soc 73 1539 1951, Beilstein 25 II 183, 25 III/IV 3727.]

5-Methylcytosine synthesis

Synthesis of 5-Methylcytosine from Thymine

5-Methylcytosine Preparation Products And Raw materials

Raw materials

5-Methyl-2-thiocytosine 5-methyl-4-(1,2,4-triazol-1-yl)-pyrimidin-2-(1H)-one 2-(Ethylthio)-5-methyl-4-pyrimidinamine 2(1H)-Pyrimidinone, 4-methoxy-5-methyl- (8CI,9CI) 4-chloro-2-ethylsulfanyl-5-methyl-pyrimidine

5-Methyl-2'-deoxycytidine 5-HydroxyMethyl cytidine 5-(Hydroxymethyl)-2'-deoxycytidine 5-Hydroxymethylcytosine (5-methyl)dithiouracil

5-formyl-2'-deoxycytidine 5-Methylcytidine Ethanol Thymine

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Preparation Products

1-[(2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione

5-Methylcytosine Suppliers

Global Suppliers ( 261)

Supplier	Tel	Email	Country	ProdList	Advantage
Tianjin Hexin Chengsai Science and Technology Development Co., Ltd.	022-23410962 13752273685	suna@hisyntech.com	China	299	58
3B Pharmachem (Wuhan) International Co.,Ltd.	18930552037	3bsc@sina.com	China	15813	69
Alfa Aesar	400-6106006	saleschina@alfa-asia.com	China	30103	84
Beijing HwrkChemical Technology Co., Ltd	89508211 18501085097	sales.bj@hwrkchemical.com	China	9409	55
Energy Chemical	400-0056266	sales8178@energy-chemical.com	China	44850	61
Nanjing Chemlin Chemical Co., Ltd	025-83697070 13770331960	info@chemlin.com.cn	China	16307	64
Shanghai Hanhong Scientific Co.,Ltd.	021-54306202 13764082696	info@hanhongsci.com	China	42917	64
China Langchem Inc.	0086-21-58956006		China	7798	57
Tianjin heowns Biochemical Technology Co., Ltd.	400 638 7771	sales@heowns.com	China	14412	57
Sinopharm Chemical Reagent Co,Ltd.	86-21-63210123	sj_scrc@sinopharm.com	China	9791	79

Supplier	Advantage
Tianjin Hexin Chengsai Science and Technology Development Co., Ltd.	58
3B Pharmachem (Wuhan) International Co.,Ltd.	69
Alfa Aesar	84
Beijing HwrkChemical Technology Co., Ltd	55
Energy Chemical	61
Nanjing Chemlin Chemical Co., Ltd	64
Shanghai Hanhong Scientific Co.,Ltd.	64
China Langchem Inc.	57
Tianjin heowns Biochemical Technology Co., Ltd.	57
Sinopharm Chemical Reagent Co,Ltd.	79

View Latest Price from 5-Methylcytosine manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2026-07-17	5-Methylcytosine 554-01-8	$29.00		99.25%	10g	TargetMol Chemicals Inc.
2021-07-13	5-methylcytosine 554-01-8	$10.00-15.00	1KG	99%+ HPLC		Zhuozhou Wenxi import and Export Co., Ltd
2021-07-10	5-methylcytosine 554-01-8	$10.00-15.00	1KG	99%+ HPLC		Zhuozhou Wenxi import and Export Co., Ltd

5-Methylcytosine
554-01-8
$29.00
99.25%
TargetMol Chemicals Inc.

5-methylcytosine
554-01-8
$10.00-15.00
99%+ HPLC
Zhuozhou Wenxi import and Export Co., Ltd

5-methylcytosine
554-01-8
$10.00-15.00
99%+ HPLC
Zhuozhou Wenxi import and Export Co., Ltd

5-Methylcytosine Spectrum

5-METHYLCYTOSINE(554-01-8)¹HNMR

554-01-8 (5-Methylcytosine) Related Search:

2'-C-Methylcytidine 1-(2-Deoxy-beta-D-ribofuranosyl)-5-methylcytosine 2-O-Methylcytosine N4-Methylcytosine ,98%,N4-Methylcytosine Cytosine

5-Methylcytidine 5-Methylcytosine 5-Carboxycytosine 5-Hydroxymethylcytosine 5-(Trifluoromethyl)-2'-deoxycytidine

5-Methylcytosine hydrochloride 5'-O-(4,4'-Dimethoxytrityl)-5-methyl-N4-benzoyl-2'-deoxycytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite 6-Methylcytosine N-Methylcytosine BIOTIN-11-DDCTP

FLUORESCEIN-12-DCTP N4-Benzoyl-5-methyldeoxycytidine 5'-Methyl-2'-deoxy-cytidine-5'-triphosphate trilithium salt

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4-Amino-5-methyl-2-pyrimidinol Cytosine, 5-methyl- 5-Methylcytosine,>96% 2(1H)-Pyrimidinone, 4-amino-5-methyl- (9CI) 4-amino-5-methyl-3H-pyrimidin-2-one 2(1H)-Pyrimidinone, 6-amino-5-methyl- 2(1H)-Oxo-4(3H)-imino-5-methylpyrimidine 2(1H)-Oxo-4-amino-5-methylpyrimidine 2-Hydroxy-4-amino-5-methylpyrimidine 4-Amino-5-methyl-1,2-dihydropyrimidine-2-one 4(6)-AMino-2-hydroxy-5-MethylpyriMidine 4-AMino-5-Methyl-2(1H)pyriMidinone 4-aMino-5-Methyl-1,2-dihydropyriMidin-2-one 5-Methylcytosine, 97%, 97% 5-methylcytosin 5-Methylcytosine (~90%) 5-METHYLCYTOSINE USP/EP/BP 4-Amino-5-methylpyrimidin-2(1H)-one 5-Methylcytosine, 10 mM in DMSO 6-amino-5-methyl-1H-pyrimidin-2-one 5-Methylcytosine 554-01-8 Nucleic acids Pyrimidines PYRIMIDINE Elisa Kit-Rat Elisa Kit