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EC 2.7.1.40

EC 2.7.1.40 structure

CAS No.: 9001-59-6

Chemical Name:: EC 2.7.1.40

Synonyms: PK;EC 2.7.1.40;pyruvic kinase;PYRUVATE KINASE;PK （rabbit muscle);Pyruvate Kinase M2;EC 2.7.1.40 USP/EP/BP;pyruvate phosphoferase;PYRUVATE KINASE TYPE I;PYRUVATE KINASE TYPE II

CBNumber:: CB9396847

Molecular Formula:: NULL

Molecular Weight:: 0

MDL Number:: MFCD00081946

MOL File:: Mol file

MSDS File:: SDS

Last updated:2024-01-10 18:10:36

Request For Quotation

EC 2.7.1.40 Properties

storage temp.	-20°C
form	powder
color	white
Water Solubility	Soluble in water ( 1mg/ml).
EPA Substance Registry System	Kinase (phosphorylating), pyruvate (9001-59-6)

SAFETY

Risk and Safety Statements

WGK Germany	3
F	10-21
TSCA	Yes
HS Code	35079090

EC 2.7.1.40 price More Price(18)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	P9136	Pyruvate Kinase from rabbit muscle Type III, lyophilized powder, 350-600?units/mg protein	9001-59-6	1000units	$79.4	2024-03-01	Buy
Sigma-Aldrich	P9136	Pyruvate Kinase from rabbit muscle Type III, lyophilized powder, 350-600?units/mg protein	9001-59-6	5000units	$199	2024-03-01	Buy
Sigma-Aldrich	SRP0414	PKLR Var2 active human recombinant,expressedinE.coli,≥90%(SDS-PAGE)	9001-59-6	20μg	$653	2024-03-01	Buy
Sigma-Aldrich	SRP0413	PKLR Var1 active human recombinant,expressedinE.coli,≥43%(SDS-PAGE)	9001-59-6	20μg	$653	2024-03-01	Buy
Sigma-Aldrich	P9136	Pyruvate Kinase from rabbit muscle Type III, lyophilized powder, 350-600?units/mg protein	9001-59-6	25000units	$602	2024-03-01	Buy

Product number	Packaging	Price	Buy
P9136	1000units	$79.4	Buy
P9136	5000units	$199	Buy
SRP0414	20μg	$653	Buy
SRP0413	20μg	$653	Buy
P9136	25000units	$602	Buy

EC 2.7.1.40 Chemical Properties,Uses,Production

Chemical Properties

White powder

Uses

Pyruvate kinase has been used in the preparation of cell free extract from BL21 CP strain of E. coli culture for highly productive cell-free protein expression. The enzyme has been used to assay the pyruvate kinase activity by incubating with eluted DAPk (Death-associated protein kinase), a serine/threonine kinase that binds and activates pyruvate kinase. It is involved in glycolysis and in gluconeogenesis. It has been used in PGM enzyme assays to determine phosphoglycerate mutase activity. It is also used to study pyruvate kinase (PK) deficiency.

Uses

Pyruvate kinase from Bacillus stearothermophilus has been used in a study to assess evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon. It has also been used in a study to investigate the importance of the Lys221 active site for pyruvate kinase activity.

Uses

Pyruvate kinase from rabbit muscle has been used in a structural study to understand the reaction mechanism of the final step in glycolysis. It has also been used in a study to investigate ATP-dependent phosphorylation of α-substituted carboxylic acids.

General Description

Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L, R, M₁, and M₂. The L isozyme is localized in gluconeogenic tissues, particularly in the liver. Whereas the R and M₁ are localized in the adult skeletal muscles, heart, brain and erythrocytes, respectively. M₂ is localized in the nucleus of the cells.

Biochem/physiol Actions

Molecular Weight: 237 kDa?and exists as a tetramer of four equal subunits of molecular weight 57 kDa.Isoelectric Point: 7.6Optimal pH: ～7.5 Optimal Temperature: 25°C ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.

Purification Methods

These are purified by (NH4)2SO4 fractionation and gel filtration, ion-exchange and affinity chromatography. [Garcia-Olalla & Garrido-Pertierra Biochem J 241 573 1987.]

EC 2.7.1.40 Preparation Products And Raw materials

Raw materials

Ammonium sulfate

Preparation Products

EC 2.7.1.40 Suppliers

Global( 142)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Chongqing Zhihe Biopharmaceutical Co., Ltd.	+86-18580541567 +86-17782035140	sales@zhswyy.com	China	338	58
Shanghai Zheyan Biotech Co., Ltd.	18017610038	zheyansh@163.com	CHINA	3620	58
career henan chemical co	+86-0371-86658258	sales@coreychem.com	China	29914	58
Chongqing Chemdad Co., Ltd	+86-023-61398051 +8613650506873	sales@chemdad.com	China	39916	58
CONIER CHEM AND PHARMA LIMITED	+8618523575427	sales@conier.com	China	49390	58
Creative Enzymes	1-516-855-7709	info@creative-enzymes.com	United States	8748	58
Hubei Xindesheng Material Technology Co., Ltd.	+8618971041571	vickyzhao@whdschem.com	China	174	58
Hefei TNJ Chemical Industry Co.,Ltd.	0551-65418671	sales@tnjchem.com	China	34572	58
HANGZHOU CLAP TECHNOLOGY CO.,LTD	86-571-88216897,88216896 13588875226	sales@hzclap.com	CHINA	6313	58
ANHUI WITOP BIOTECH CO., LTD	+8615255079626	eric@witopchemical.com	China	23556	58

Supplier	Advantage
Chongqing Zhihe Biopharmaceutical Co., Ltd.	58
Shanghai Zheyan Biotech Co., Ltd.	58
career henan chemical co	58
Chongqing Chemdad Co., Ltd	58
CONIER CHEM AND PHARMA LIMITED	58
Creative Enzymes	58
Hubei Xindesheng Material Technology Co., Ltd.	58
Hefei TNJ Chemical Industry Co.,Ltd.	58
HANGZHOU CLAP TECHNOLOGY CO.,LTD	58
ANHUI WITOP BIOTECH CO., LTD	58

View Lastest Price from EC 2.7.1.40 manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2024-04-26	EC 2.7.1.40 9001-59-6	US $0.00-0.00 / kg	0.10000000149011612kg	≥99%	20tons	Chongqing Zhihe Biopharmaceutical Co., Ltd.
2023-08-03	Pyruvate Kinase 9001-59-6	US $12.00-9.00 / KU	10KU	≥ 25.0U/mg	10000ku	Hubei Xindesheng Material Technology Co., Ltd.
2021-09-29	Pyruvate Kinase 9001-59-6	US $0.00-0.00 / g	1g	98.0%	1kg/month	WUHAN FORTUNA CHEMICAL CO., LTD

EC 2.7.1.40
9001-59-6
US $0.00-0.00 / kg
≥99%
Chongqing Zhihe Biopharmaceutical Co., Ltd.

Pyruvate Kinase
9001-59-6
US $12.00-9.00 / KU
≥ 25.0U/mg
Hubei Xindesheng Material Technology Co., Ltd.

Pyruvate Kinase
9001-59-6
US $0.00-0.00 / g
98.0%
WUHAN FORTUNA CHEMICAL CO., LTD

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ATP:PYRUVATE 2-O-PHOSPHOTRANSFERASE ATP: PYRUVATE O2-PHOSPHOTRANSFERASE EC 2.7.1.40 PK （rabbit muscle) pyruvate phosphoferase PYRUVATE KINASE FROM RABBITMUSCLE CA. 350 U/MG PROTEIN SUSP. Pyruvate Kinase from Bacillus stearothermophilus,ATP:pyruvate 2-O-phosphotransferase, PK Pyruvate Kinase from rabbit muscle,ATP:pyruvate 2-O-phosphotransferase, PK PYRUVATE KINASE, PRESTAINED FROM*CHICKEN MUSCLE PK(R.M.), ATP: pyruvate 2-o-phosphotransferase Pyruvate kinase (from Rabbit muscle), ammonium sulfate suspension Pyruvat kinase from rabbit, lyophil. PHOSPHOENOL PYRUVATE KINASE PK PYRUVATE KINASE PYRUVATE KINASE, RABBIT MUSCLE PYRUVATE KINASE TYPE I PYRUVATE KINASE TYPE II PYRUVATE KINASE TYPE III PYRUVATE KINASE TYPE VIII PYRUVATE O2-PHOSPHO TRANSFERASE Pyruvate Kinase, 250 units/Mg Pyruvate Kinase froM MicroorganisM Pyruvate Kinase from rabbit liver pyruvate kinase from rabbit muscle pyruvate kinase from rabbit muscle cryst susp. ~350 U/mg Pyruvate Kinase preparation from rabbit muscle pyruvate kinase type I crude*from rabbit muscle pyruvate kinase type iii from rabbit*muscle pyruvate kinase type viii from*bacillus stearothe pyruvate kinase type X from chicken*muscle pyruvate kinase type xii from rabbit*liver Kinase (phosphorylating), pyruvate PYRUVATE KINASE TYPE X FROM CHICKEN & PYRUVATE KINASE TYPE VII FROM*RABBIT MUS CLE PYRUVATE KINASE PRESTAINED FROM CHICKEN PYRUVATE KINASE FROM RABBIT MUSCLE, 100- 250 U/MG PYRUVATE KINASE TYPE VIII FROM*BACILLUS STEAROTHERM PYRUVATE KINASE TYPE II FROM RABBIT*MUSC LE PYRUVATE KINASE (EC 2.7.1.40) FROM RABBIT MUSCLE LYOPHILIZED POWDER ESSENTIALLY SALT-FREE PYRUVATE KINASE FROM PORCINE HEART (E.C. 2.7.1.40) PYRUVATE KINASE (EC 2.7.1.40) FROM RABBIT MUSCLE HIGHLY PURIFIED SUSPENSION PYRUVATE KINASE FROM BACILLUS STEAROTHERMOPHILUS PyruvateKinaseExRabbitMuscle Pyruvate kinase from rabbit muscle ca. 350 U/mg protein pyruvic kinase ATP:pyruvate 2-O-phosphotransferase, PK Pyruvate Kinase preparation from canine muscle phosphopyruvate transphos phorylase Native Bacillus stearothermophilus Pyruvate Kinase EC 2.7.1.40 USP/EP/BP Pyruvate Kinase ex. Rabbit Muscle, 300U/mg Pyruvate Kinase, 50 units/mg Pyruvate Kinase M2 from Human, Recombinant Pyruvate kinase M2 Active from Mouse, Recombinant Native Pyruvate Kinase from Thermophillic bacteria Pyruvate Kinase M2 9001-59-6