Company Name: |
Creative Enzymes
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Tel: |
1-516-855-7709 |
Email: |
info@creative-enzymes.com |
Products Intro: |
Product Name:keratan-sulfate endo-1, 4-β -galactosidase CAS:55072-01-0
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| KERATANASE Basic information |
Product Name: | KERATANASE | Synonyms: | ENDO-BETA-GALACTOSIDASE;EC 3.2.1.103;KERATANASE;KERATANASE, PSEUDOMONAS SPECIES;Endo-b-galactosidase, Escherichia freundii;keratanase from pseudomonas species*10 units per;Keratanase, Pseudomonas sp. *Not for sale in Japan*;KERATANASE FROM PSEUDOMONAS SPECIES*10 U NITS PER VI | CAS: | 55072-01-0 | MF: | | MW: | 0 | EINECS: | | Product Categories: | | Mol File: | Mol File | ![KERATANASE Structure]() |
| KERATANASE Chemical Properties |
storage temp. | 2-8°C | form | lyophilized powder |
| KERATANASE Usage And Synthesis |
Uses | Endo-β-galactosidase was used in fractional protein isolation. It was used for deglycosylation in glycoproteomics of the endothelial secretome of human endothelial cells. | Biochem/physiol Actions | Internal β(1-4) galactose linkages in unbranched, repeating poly-Nacetyllactosamine [GlcNAc β(1-3)Gal β (1-4)] structures are the preferred substrate.Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may reduce or completely inhibit cleavage. Sulfation of C-6 on galactose will block cleavage. Oligosaccharides of the neolacto-group are cleaved at greatly reduced rates depending on the deviation from the preferred substrate. For example, Gal β(1-3)GlcNAc β(1-3) Gal β(1-4)Glc is cleaved at 5X10-5 the rate of keratan sulfateβ-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop. |
| KERATANASE Preparation Products And Raw materials |
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