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Trypsin Inhibitor, pankreatischbasisch Produkt Beschreibung

Aprotinin Struktur
9087-70-1
CAS-Nr.
9087-70-1
Bezeichnung:
Trypsin Inhibitor, pankreatischbasisch
Englisch Name:
Aprotinin
Synonyma:
BPTI;fosten;rp-9921;TRASYLOL;onquinin;repulson;riker52g;trazinin;zymofren;antilysin
CBNumber:
CB3329436
Summenformel:
C284H432N84O78R2S7
Molgewicht:
6495.43988
MOL-Datei:
9087-70-1.mol

Trypsin Inhibitor, pankreatischbasisch Eigenschaften

storage temp. 
2-8°C
Löslichkeit
glycerol: soluble3mg/mL, clear, colorless (equilibration buffer containing 5% glycerol)
Aggregatzustand
lyophilized powder
Farbe
white
Wasserlöslichkeit
Freely soluble in water and in aqueous buffers of low ionic strength.
Merck 
13,761
Stabilität:
Stable. Incompatible with strong oxidizing agents.

Sicherheit

Kennzeichnung gefährlicher Xn,Xi
R-Sätze: 42/43-36/37/38-20/21/22
S-Sätze: 22-45-36/37-36-26
WGK Germany  1
RTECS-Nr. YN5080000
10-21
HS Code  35040000
Giftige Stoffe Daten 9087-70-1(Hazardous Substances Data)
Toxizität LD50 i.v. in mice: 2500000 kallikrein inhibitor units/kg (Trautschold)

Trypsin Inhibitor, pankreatischbasisch Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:

R42/43:Sensibilisierung durch Einatmen und Hautkontakt möglich.
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
R20/21/22:Gesundheitsschädlich beim Einatmen,Verschlucken und Berührung mit der Haut.

S-Sätze Betriebsanweisung:

S22:Staub nicht einatmen.
S45:Bei Unfall oder Unwohlsein sofort Arzt zuziehen (wenn möglich, dieses Etikett vorzeigen).
S36/37:Bei der Arbeit geeignete Schutzhandschuhe und Schutzkleidung tragen.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.

Beschreibung

Aprotinin is a reversible inhibitor of serine proteases such as trypsin (Ki = 0.06 pM), chymotrypsin (Ki = 9.5 nM), and kallikrein (Ki = 0.8 nM). It is a much weaker inhibitor of thrombin (Ki = 0.1-0.8 mM) and trypsinogen (Ki = 2 μM). Aprotinin also competitively inhibits nNOS and iNOS with Ki values of 50 and 78 μM, respectively. Aprotinin is widely used as a protein purification tool to prevent proteases present in tissue samples from degrading the protein of interest.

Chemische Eigenschaften

white powder

Originator

Trasylol,Bayer

Verwenden

Aprotinin is largely used as an inhibitor of trypsin.

Verwenden

Proteolytic inhibitor in radioimmunoassays of polypeptide hormones.

Verwenden

A potent protease inhibitor.Aprotinin acts as a reversible serine protease blocking and a binding agent. It is widely employed as an inhibitor of trypsin. It is also used as a proteolytic inhibitor in radioimmunoassays of polypeptide hormones. It is involved in the purification of urokinase, trypsin, and chymotrypsin on immobilized aprotinin. Further, it is used in the quantification of kallikrein activity in mixtures of esterases and proteases.

Manufacturing Process

1 kg of parotid of cattle, which were freed of fat and flesh are comminuted in a meat chopper and twice extracted with 5 L of acetone. The acetone was removed as far as possible by sucking off and the moist material is digested for 2.5 hours in 1.2 L of 1 N acetic acid and 2.8 L of 96% ethanol at 50°C. The filtrate containts about 230.000 inactivator units.
The alcoholic solution is concentrated to 1.2 L by evaporating in vacuo and shaken with an equal amount of ether. Dark coloring matters and residues of fat are thus dissolved. The aqueous phase is now mixed with acetone until a precipitate occurs and the dissolved in dilute acetic acid. The solution is brought to a weakly alkaline pH by addition of ammonia. It is then centrifuged and the inactivator is precipitated with five times the amount of ethanol. The yield amounts to about 180.000 kallikrein inactivator units in 0.4-0.5 g of substance.

Trademarks

Trasylol (Bayer).

Therapeutic Function

Proteinase inhibitor

Allgemeine Beschreibung

Trypsin inhibitor, pancreas type from bovine lung. It is known as Pancreatic trypsin inhibitor (BPTI). Aprotinin, also known as pancreatic trypsin inhibitor and trypsin-kallikrein inhibitor, is found to be expressed in lungs, spleen, liver, and pancreas. It is also found to be present in the free form in calf serum.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

Sicherheitsprofil

Experimental reproductive effects. When heated to decomposition it emits toxic fumes of NOx and SOx.

Trypsin Inhibitor, pankreatischbasisch Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


Trypsin Inhibitor, pankreatischbasisch Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global( 260)Lieferanten
Firmenname Telefon Fax E-Mail Land Produktkatalog Edge Rate
Henan Tianfu Chemical Co.,Ltd.
0371-55170693
0371-55170693 info@tianfuchem.com China 22607 55
Hefei TNJ Chemical Industry Co.,Ltd.
+86-0551-65418679
86-0551-65418697 info@tnjchem.com China 3000 55
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career henan chemical co
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BOC Sciences
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Creative Enzymes
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Target Molecule Corp
18019718960 781-999-5354
marketing@targetmol.com United States 19231 58

9087-70-1(Trypsin Inhibitor, pankreatischbasisch)Verwandte Suche:


  • TRYPSIN INHIBITOR, PANCREAS TYPE
  • TRYPSIN INHIBITOR (BASIC)
  • TRYPSIN INHIBITOR, BOVINE LUNG
  • TRASYLOL
  • antikrein
  • antilysin
  • antilysine
  • aprotininfrombovinelung
  • basicpancreatictrypsininhibitor
  • RecoMbinant aprotinin
  • APROTININ-AGAROSE
  • APROTININ, BOVINE
  • APROTININ, BOVINE AGAROSE CONJUGATE
  • APROTININ BOVINE LUNG
  • Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free - CAS 9087-70-1 - Calbiochem
  • Aprotinin froM bovine lung, ca. 140 U/Mg lyophil.
  • Monoclonal Anti-Acetylated Lysine antibody produced in mouse
  • Anti-Acetylated Lysine: Biotin
  • Anti-Acetylated Lysine-Biotin antibody produced in rabbit
  • Anti-Acetylated Lysine antibody produced in rabbit
  • Aprotinin from bovine lung, >=5400 KIU/mg
  • EZNA KIT ENDO-FREE PLASMID GIGA
  • H-ARG-PRO-ASP-PHE-CYS-LEU-GLU-PRO-PRO-TYR-THR-GLY-PRO-CYS-LYS-ALA-ARG-ILE-ILE-ARG-TYR-PHE-TYR-ASN-ALA-LYS-ALA-GLY-LEU-CYS-GLN-THR-PHE-VAL-TYR-GLY-GLY-CYS-ARG-ALA-LYS-ARG-ASN-ASN-PHE-LYS-SER-ALA-GLU-ASP-CYS-MET-ARG-THR-CYS-GLY-GLY-ALA-OH
  • EC: 3.4.21.9
  • BPTI
  • APROTININ
  • bayera-128
  • fosten
  • kallikrein-trypsininactivator
  • kirrichter
  • onquinin
  • pancreaticbasictrypsininhibitor
  • pancreatictrypsininhibitor
  • pancreatictrypsininhibitor(kunitz)
  • repulson
  • riker52g
  • rp-9921
  • trazinin
  • trypsininhibitor,pancreaticbasic
  • trypsin-kallikreininhibitor(kunitz)
  • zymofren
  • Aprotinin solution
  • Anti-Acetylated Lysine:HRP
  • Anti-Acetylated Lysine-Peroxidase antibody produced in rabbit
  • APROTININ FROM BOVINE LUNGAFFINITY PURIF IED
  • APROTININ FROM BOVINE LUNG CELL CULTURE TESTED
  • APROTININ FROM BOVINE LUNG, ~8000 U/MG P ROTEIN
  • APROTININ, BOVINE LUNG FOR GELFILTRATION CHROMATOG
  • APROTININ-AGAROSE FROM BOVINE LUNG
  • APROTININ, BOVINE, RECOMBINANT
  • APROTININ FROM BOVINE LUNG, LYOPH.,~7000 U/MG*
  • Aprotinin from bovine lung, ca. 140 U/mg
  • Antilysi.
  • Trypsin Inhibitor, pankreatisch basisch
  • Bovine pancreatic trypsin inhibitor
  • Kunitz protease inhibitor
  • Aids043659
  • Aids-043659
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