ChemicalBook
Chinese Japanese Germany Korea

ASPARAGINASE

Description References
ASPARAGINASE
ASPARAGINASE structure
CAS No.
9015-68-3
Chemical Name:
ASPARAGINASE
Synonyms
CL059;mk-965;elspar;LASPAR;leunase;paronal;l-asnase;colaspase;crasnitin;kidrolase
CBNumber:
CB2484567
Molecular Formula:
C14H17NO4S
Formula Weight:
295.35
MOL File:
Mol file

ASPARAGINASE Properties

alpha 
D20 -30 to -32°
storage temp. 
2-8°C
form 
suspension
Merck 
13,841
CAS DataBase Reference
9015-68-3
SAFETY
  • Risk and Safety Statements
  • Hazard and Precautionary Statements (GHS)
Hazard Codes  T,Xn
Risk Statements  61-42/43-63
Safety Statements  53-22-36/37/39-45-36/37
RIDADR  3249
WGK Germany  3
RTECS  CI9000000
10-21
HazardClass  6.1(a)
PackingGroup  II
Symbol(GHS):
Signal word: Warning
Hazard statements:
Code Hazard statements Hazard class Category Signal word Pictogram P-Codes
H317 May cause an allergic skin reaction Sensitisation, Skin Category 1 Warning P261, P272, P280, P302+P352,P333+P313, P321, P363, P501
H361 Suspected of damaging fertility or the unborn child Reproductive toxicity Category 2 Warning P201, P202, P281, P308+P313, P405,P501
Precautionary statements:
P280 Wear protective gloves/protective clothing/eye protection/face protection.

ASPARAGINASE price More Price(2)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich A3809 Asparaginase from Escherichia coli lyophilized powder, 100-300 units/mg protein (biuret) 9015-68-3 100un $225 2018-11-13 Buy
Sigma-Aldrich A3809 Asparaginase from Escherichia coli lyophilized powder, 100-300 units/mg protein (biuret) 9015-68-3 1ku $1080 2018-11-13 Buy

ASPARAGINASE Chemical Properties,Uses,Production

Description

Asparaginase is a kind of enzyme that can be used as a medication and in food industry. It is mainly extracted from E. coli. In the medical field, it can be used for the treatment of acute lymphoblastic leukemia, acute myeloid leukemia, and non-Hodgkin’s lymphoma. This is due to that asparaginase can convert the L-asparagine into aspartate and ammonia, exhausting the available asparagine needed for leukemic cells and thus leading to cell death. In the food industry, it can be used as a processing aid in the manufacture of food to reduce the formation of acrylamide, which is a potential carcinogen through removing the asparagine which can undergo Maillard reaction during cooking to induce tumor in fried and baked food.

References

https://en.wikipedia.org/wiki/Asparaginase

Description

Using the enzyme asparaginase (L-asparagine amidohydrolase, EC 3.5.1.1), it is possible to significantly reduce the formation of the cooking carcinogen acrylamide during roasting, deep-frying, or baking of foods. The enzyme hydrolyzes free asparagine to aspartic acid, thereby preventing the formation of acrylamide by reaction of asparagine with reducing sugars at elevated temperatures during the Maillard reaction.
The mitigation of acrylamide formation is especially important for a number of cereal- and potato-based products, including crackers, crispbread, gingerbread, biscuits, French fries, and potato chips. After asparaginase pretreatment, the acrylamide concentration of certain foods could be reduced by up to 97 % [84, 86]. By means of in vitro directed evolution, the properties of asparaginase were optimized. For example, an Asp133Leu mutation of a wild-type enzyme showed a significantly improved thermal stability. The enzyme’s half-life at 50 C increased from 3 to 160 h, and the half-inactivation temperature of the mutant was 9 C higher.

Originator

Enzon (U.S.A.)

Uses

Antineoplastic.

Definition

An enzyme used in the treatment of certain types of leukemia. Produced by biochemical activity of certain bacteria, yeasts, and fungi. Yields are in excess of 3500 units/g of source.

brand name

Crasnitin (Bayer); Elspar (Merck);Oncaspar.

General Description

Asparaginase is available in 10-mL vials for intramuscularand IV use in the treatment of acute lymphocytic leukemia.
Tumor cells are unable to synthesize asparagine, and thereforemust utilize what is available in the extracellular environment.The agent acts by hydrolyzing extracellular asparagineto aspartate and ammonia. The tumor cells are then deprivedof a necessary nutrient, and protein synthesis is inhibited leadingto cell death. The agent is specific for the G1 phase of thecell cycle. Resistance occurs because of the development ofthe tumor cells ability to produce asparagine synthetase thatallows them to synthesize the required amino acid. Antibodyproduction directed at asparaginase may be stimulated by theagent as well. The agent remains in the extracellular spaceafter parental administration and is 30% protein bound. Themetabolism of the agent has not been well characterized andthe plasma half-life depends on the formulation of the drug.The E. coli-derived agent has a plasma half-life of 40 to 50hours, whereas polyethylene glycol-asparaginase’s half-life is3 to 5 days. Adverse effects include hypersensitivity reactions,fever, chills, nausea, lethargy, confusion, hallucinations,and possibly coma. Myelosuppression is not generallyseen. An increased risk of bleeding and clotting is seen in halfof the patients taking the agent.

Biotechnological Applications

L-asparaginase (EC. 3.5.1.1; asparagine amidohydrolase) catalyzes the hydrolysis of L-Asparagine to L-aspartic acid and ammonia. This enzyme is used for the treatment of selected types of hemopoietic diseases such as acute lymphoblastic leukemia (ALL) and non-Hodgkin lymphomas (Pieters et al. 2011; Rytting 2012). It is also a model enzyme for the development of new drug delivery system and L-asparagine biosensor for leukemia. This enzyme was used in the food industry for the production of acrylamide free food (Kumar and Verma 2012; Dhanam Jayam and Kannan 2013).
Y. lipolytica is a potential producer of L-asparaginase. However, there are very few reports on L-asparaginase production by the yeast. Karanam and Medicherla optimized L-asparaginase production of Y. lipolytica NCIM 3472 in solid-state fermentation (SSF) using palm kernel cake as the substrate. The maximum L-asparaginase activity at optimum conditions was near 40 U/g of the initial dry substrate (U/gds) (Karanam and Medicherla 2010).

Clinical Use

Pegaspargase, a polyethylene glycol conjugate of L-asparaglnase (ASNase), was launched for combination chemotherapy in acute lymphoblastic leukemia (ALL). L-Asparaginase is an enzyme that inhibits protein synthesis by the depletion of sources of L-asparagine, which is necessary for transformed lymphoid cells to proliferate. It has been used as a standard component of the antileukemia armamentarium for childhood All. Pegaspargase has greater antitumor activity, a longer plasma half-life and less immunogenicity than ASNase. It produces minimal side effects after repeated dosing, whereas ASNase induces anaphylactic shock, urticaria, anorexia or vomiting and acute pancreatitis in dogs, and other immunological effects in man resulting from sensitization to the enzyme or protein synthesis inhibition. The efficacy of pegaspargase for other indications including breast and lung cancers, non-Hodgkin's lymphoma and pancreatic cancer has been reported.

Anticancer Research

Both the commonly known and distinctive species have been reported to produceL-asparaginase. The common species of endophytes, which produce L-asparaginase,include Fusarium sp., Penicillium sp., and Colletotrichum sp. They are isolated asendophytes from a variety of medicinal plants (Audipudi et al. 2014; El-Said et al.2016).
Chow and Ting (2015) studiedL-asparaginase production from fungal endophytes isolated from anticancer plantsin Malaysia. They found Fusarium oxysporum and Penicillium simplicissimumfrom Murraya koenigii and Pereskia bleo, respectively, as effective producers ofL-asparaginase. In addition to L-asparaginase, endophytes from anticancer plantshave also been established as producers of other valuable anticancer, antimicrobial,and antioxidant compounds. This is further supported by many reports on discoveryof these anticancer agents in different species of endophytic fungi either from sameor different host plants.

ASPARAGINASE Preparation Products And Raw materials

Raw materials

Preparation Products


ASPARAGINASE Suppliers

Global( 65)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Henan DaKen Chemical CO.,LTD.
+86-371-55531817
info@dakenchem.com CHINA 21990 58
Henan Tianfu Chemical Co.,Ltd.
0371-55170693
0371-55170693 info@tianfuchem.com CHINA 20680 55
Chemwill Asia Co.,Ltd.
86-21-51086038
86-21-51861608 chemwill_asia@126.com;sales@chemwill.com;chemwill@hotmail.com;chemwill@gmail.com CHINA 24001 58
career henan chemical co
+86-371-86658258
sales@coreychem.com CHINA 19923 58
LGM Pharma 1-(800)-881-8210
615-250-9817 inquiries@lgmpharma.com United States 1943 70
Tianjin heowns Biochemical Technology Co., Ltd. 400 638 7771
sales@heowns.com China 14464 57
ShangHai YuanYe Biotechnology Co., Ltd. 021-61312847 qq:2797782341
021-55068248 2797782341@qq.com China 7571 60
Beijing HuaMeiHuLiBiological Chemical 010-56205725;010-86181995
010-65763397 waley188@sohu.com China 12343 58
Guangzhou Isun Pharmaceutical Co., Ltd 020-39119399 18927568969
020-39119999 isunpharm@qq.com, isunpharm@hotmail.com China 5020 55
Nanjing Sunlida Biological Technology Co., Ltd. 025-58378339;025-57798810
025-57019371 sales@sunlidabio.com China 3823 55

View Lastest Price from ASPARAGINASE manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2018-12-24 ASPARAGINASE
9015-68-3
US $1.00 / kg 1kg 95%-99% 100kg career henan chemical co
2018-12-24 ASPARAGINASE
9015-68-3
US $1.00 / kg 1kg 95%-99% 100kg career henan chemical co

9015-68-3(ASPARAGINASE)Related Search:


Copyright 2017 © ChemicalBook. All rights reserved