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DL-Dithiothreitol

Common reductant Uses
DL-Dithiothreitol
DL-Dithiothreitol structure
CAS No.
3483-12-3
Chemical Name:
DL-Dithiothreitol
Synonyms
DTT;DITT;D-Dtt;DL-DTT;1M DTT;wr34678;DTT-MBG;CGMP DTT;DTT, 10G;DTT, 25G
CBNumber:
CB6342919
Molecular Formula:
C4H10O2S2
Formula Weight:
154.25
MOL File:
3483-12-3.mol

DL-Dithiothreitol Properties

Melting point:
41-44 °C(lit.)
Boiling point:
125 °C
alpha 
-0.2~+0.2°(20℃/D)(c=5,H2O)
Density 
1.04 g/mL at 20 °C
vapor density 
5.3 (vs air)
refractive index 
1.5200 (estimate)
Flash point:
>230 °F
storage temp. 
2-8°C
solubility 
H2O: 50 mg/mL, clear, colorless
form 
Powder
pka
pK1:8.9 (25°C)
color 
White
PH
4.0-6.0 (20-25℃, 0.1m in H2O)
Odor
Unpleasant Odor
PH Range
4 - 6 at 15,4 g/l at 25 °C
Water Solubility 
freely soluble
Sensitive 
Air Sensitive
λmax
λ: 260 nm Amax: 0.400
λ: 280 nm Amax: 0.100
Merck 
14,3376
BRN 
1719757
Stability:
Stability Stable, but heat sensitive. Incompatible with strong oxidizing agents. Keep frozen at -20 to -10 C.
InChIKey
VHJLVAABSRFDPM-UHFFFAOYSA-N
CAS DataBase Reference
3483-12-3(CAS DataBase Reference)
EWG's Food Scores
1
FDA UNII
T8ID5YZU6Y
NIST Chemistry Reference
2,3-Butanediol, 1,4-dimercapto-, (r*,r*)-(3483-12-3)
EPA Substance Registry System
1,4-Dithiothreitol (3483-12-3)
SAFETY
  • Risk and Safety Statements
Symbol(GHS) 
GHS07
Signal word  Warning
Hazard statements  H335-H302-H315-H319-H412
Precautionary statements  P302+P352+P332+P313+P362+P364-P305+P351+P338+P337+P313-P261-P280a-P304+P340-P405-P501a-P264-P270-P273-P280-P337+P313-P501-P301+P312+P330-P305+P351+P338
Hazard Codes  Xn
Risk Statements  25-36/37/38-22-20/21/22
Safety Statements  26-45-37/39-36
RIDADR  UN 3335
WGK Germany  3
RTECS  EK1610000
3-10-16-23
TSCA  Yes
HS Code  29309099
NFPA 704
1
2 1

DL-Dithiothreitol price More Price(123)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich DTT-RO DTT 1,4-Dithiothreitol 3483-12-3 2 g $77.1 2021-12-16 Buy
Sigma-Aldrich DTT-RO DTT 1,4-Dithiothreitol 3483-12-3 25 g $473 2021-12-16 Buy
Sigma-Aldrich 233155 Cleland’s Reagent - CAS 3483-12-3 - Calbiochem 3483-12-3 25 g $478.22 2021-12-16 Buy
Sigma-Aldrich 233153-M Cleland’s Reagent, ULTROL Grade - CAS 3483-12-3 - Calbiochem A protective agent for SH groups Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%. 3483-12-3 10 g $511.52 2021-12-16 Buy
Sigma-Aldrich 233155 Cleland’s Reagent - CAS 3483-12-3 - Calbiochem 3483-12-3 100 g $1147 2021-12-16 Buy

DL-Dithiothreitol Chemical Properties,Uses,Production

Common reductant

DL-1, 4-DL-1, 4-Dithiothreitol is a common reducing agent, also known as DTT with a strong reduction capability. Its reduction capability is largely due to the conformational stability of the six-membered ring (containing disulfide bone) of its oxidation state. The reducing capability is affected by the pH value and also takes effect only in the case of a pH being greater than 7. This is because it is only the deprotonated thiolate anion (-S-) that is actively in reduction reaction while thiol (an-SH) is not; and the pKa of mercapto group is typically around~8.3.
The reduction of the disulfide bond mediated by DL-1, 4-Dithiothreitol mainly consists of a two-step continuous sulfhydryl-disulfide exchange: wherein the intermediate state formed by the first step of the reaction is very unstable because the secondary sulfhydryl group of DTT tends to connected by the oxidized sulfur atom, making the intermediate state be rapidly converted to the cyclic oxidation structure of DTT, thus completing the reduction of disulfide bonds.
Since being easily oxidized by air, the stability of DL-1, 4-Dithiothreitol (DTT) is relatively poor; frozen storage or processing in inert gas can extend its duration life. Owing to the low nucleophilicity of the protonated sulfur, with the decreased pH value, the effective reducing capability of DTT is also reduced; Moreover, Tris (2-carboxyethyl) phosphine HCl (TCEP hydrochloride) can be used as the substitute of DTT at low pH value and is also more stable than DTT.
DL-1, 4-Dithiothreitol has an antioxidant property that can protect the reductive groups on the enzyme molecules and maintain a reducing environment and stabilize the enzyme activity. DTT also contain certain RNase inhibitors which can inhibit the enzymatic activity of RNase. It has a similar effect as mercaptoethanol (b-mercaptoethanol) but the irritating odor and toxicity of DTT is much smaller and lower than the toxicity of mercaptoethanol. Moreover, DTT has a similar effect as mercaptoethanol even at a concentration of being seven times lower, but the price of DTT is slightly higher. It can be used as the protein thiol group protecting agent and the cleavage of the protein disulfide bond as well as sequence analysis.
Current implemented law regarding the poisons and toxic materials has designated β-mercaptoethanol and reagent-containing β-mercaptoethanol as poison. DL-1, 4-Dithiothreitol has similar effect as β-mercaptoethanol and can protect the SH group of protein, cutting the disulfide bond and therefore will be gradually paid attention by related applications.
The above information is edited by the chemicalbook of Dai Xiongfeng.

Uses

Molecular Biology

Chemical Properties

White crystalline powder

Uses

Dithiothreitol is a redox reagent commonly used as a reducing agent for thiolated DNA. Dithiothreitol is also used to reduce the disulfide bonds of proteins.

Uses

DL-dithiothreitol (DTT) is a sulfhydryl compound that acts both as a reagent reducing disulfide bonds and as a protein denaturant on staphylococcal biofilm (Wu et al. 2011). Thanks to these properties, DTT is routinely used in clinical microbiology to liquefy respiratory specimens, but it has also been proven effective to detach biofilm from orthopaedic prosthesis (Drago et al. 2012, 2013). Since it is crucial to discriminate implant-related infections from aseptic loosening in orthopaedics, and because of the difficulty to diagnose subclinical infections, a prompt diagnosis of indwelling device-related infection is important for successful treatments (Borens et al. 2013). A fast microbiological diagnosis and bacterial identification should be recommended in order to set up a specific antimicrobial therapy. According to this strategy, the bacterial detachment from biofilm on explants should be accelerated by supporting the pathogen viability.

Uses

DL-Dithiothreitol has been added to the samples before loading them for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It has been used in the procedure of in situ hybridization of mRNA.

Definition

ChEBI: The threo-diastereomer of 1,4-dimercaptobutane-2,3-diol.

Application

DL-Dithiothreitol (DTT) is a chemical debonding technique that also has the goal of driving bacte-ria out of biofilms. A potential advantage of DTT compared with sonication is that tissue can also be evaluated without implants. Drago and col-leagues compared DTT with sonication in patients with prosthetic joint infection or aseptic loosening. Sensitivity was better with DTT (85.7%) versus sonication (71.4%), whereas both had identical specificity (94.1%). DTT had better sensitivity and specificity compared with standard tissue culture. A recent study supports the potential cost benefits of both DTT and son-ication compared with standard culture.

General Description

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Biological Activity

Reagent for maintaining SH groups in the reduced state.

Biochem/physiol Actions

DL-Dithiothreitol denatures proteins by reducing the disulphide bonds and reducing them to SH groups. It also prevents intramolecular and intermolecular disulfide bond formation between cysteine residues of proteins.

DL-Dithiothreitol Preparation Products And Raw materials

Raw materials

Preparation Products


DL-Dithiothreitol Suppliers

Global( 478)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Yunbio Tech Co.,Ltd.
010-60605551
010-60605551 yunbiochem@126.com CHINA 258 58
Casorganics US Corp
+17326109938
sales@casorganics.com CHINA 175 58
Henan DaKen Chemical CO.,LTD.
+86-371-66670886
info@dakenchem.com China 15421 58
Henan Tianfu Chemical Co.,Ltd.
0371-55170693
0371-55170693 info@tianfuchem.com China 22607 55
Hangzhou FandaChem Co.,Ltd.
008615858145714
+86-571-56059825 fandachem@gmail.com CHINA 9150 55
Hefei TNJ Chemical Industry Co.,Ltd.
+86-0551-65418679
86-0551-65418697 info@tnjchem.com China 3000 55
Shanghai Zheyan Biotech Co., Ltd.
18017610038
zheyansh@163.com CHINA 3623 58
career henan chemical co
+86-0371-55982848
sales@coreychem.com China 29953 58
Zhejiang ZETian Fine Chemicals Co. LTD
18957127338
stella@zetchem.com China 2008 58
SHANDONG ZHI SHANG CHEMICAL CO.LTD
+86 18953170293
+86 0531-67809011 sales@sdzschem.com CHINA 2940 58

View Lastest Price from DL-Dithiothreitol manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2022-01-21 1,4-dithiothreitol
3483-12-3
US $20.00 / Kg/Drum 25Kg/Drum 99.99% 1 ton per week Wuhan Mulei New Material Technology Co. Ltd
2022-01-15 dithiothreitol
3483-12-3
US $3.00 / KG 1KG 99.% 50 ton Hebei Crovell Biotech Co Ltd
2021-11-30 DL-Dithiothreitol
3483-12-3
US $1970.00 / KG 1KG 98% 1-1000kg Baoji Guokang Bio-Technology Co., Ltd.

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