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Guanidine hydrochloride

description Protein Denaturation Uses Drug intermediates Preparation Reference quality standards References
Guanidine hydrochloride
Guanidine hydrochloride structure
Chemical Name:
Guanidine hydrochloride
GUHY;GdmCl;usafek-749;Guanidine HCI;GUANIDINE HCL;Guanidine hydr;guanidinechloride;GUANIDIUM CHLORIDE;AMINOFORMAMIDINE HCL;Guanidine Hydrochlor
Molecular Formula:
Formula Weight:
MOL File:

Guanidine hydrochloride Properties

Melting point:
180-185 °C(lit.)
1.18 g/mL at 25 °C(lit.)
refractive index 
n20/D 1.465
storage temp. 
Store at RT.
H2O: 6 M, clear, colorless
White to slightly yellow
4.5-5.5 (100g/l, H2O, 20℃)
Water Solubility 
2280 g/L (20 ºC)
λ: 260 nm Amax: 0.040
λ: 280 nm Amax: 0.025
Stable. Hygroscopic. Incompatible with strong oxidizing agents.
CAS DataBase Reference
50-01-1(CAS DataBase Reference)
EPA Substance Registry System
Guanidine, monohydrochloride(50-01-1)
  • Risk and Safety Statements
  • Hazard and Precautionary Statements (GHS)
Hazard Codes  Xn,Xi
Risk Statements  22-36/38
Safety Statements  26-36-22
WGK Germany  1
RTECS  MF4300000
HS Code  29252000
Toxicity LD50 orally in Rabbit: 655.3 - 907.1 mg/kg LD50 dermal Rabbit > 2000 mg/kg
Signal word: Warning
Hazard statements:
Code Hazard statements Hazard class Category Signal word Pictogram P-Codes
H302 Harmful if swallowed Acute toxicity,oral Category 4 Warning P264, P270, P301+P312, P330, P501
H315 Causes skin irritation Skin corrosion/irritation Category 2 Warning P264, P280, P302+P352, P321,P332+P313, P362
H319 Causes serious eye irritation Serious eye damage/eye irritation Category 2A Warning P264, P280, P305+P351+P338,P337+P313P
Precautionary statements:
P261 Avoid breathing dust/fume/gas/mist/vapours/spray.
P280 Wear protective gloves/protective clothing/eye protection/face protection.
P321 Specific treatment (see … on this label).
P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P332+P313 IF SKIN irritation occurs: Get medical advice/attention.
P337+P313 IF eye irritation persists: Get medical advice/attention.

Guanidine hydrochloride price More Price(58)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 5010-OP Guanidine hydrochloride 50-01-1 1kg $337 2018-11-20 Buy
Sigma-Aldrich 50933 Guanidine hydrochloride BioUltra, for molecular biology, ≥99.5% (AT) 50-01-1 1kg $2250 2018-11-13 Buy
TCI Chemical G0162 Guanidine Hydrochloride >98.0%(T) 50-01-1 25g $22 2018-11-22 Buy
TCI Chemical G0162 Guanidine Hydrochloride >98.0%(T) 50-01-1 500g $131 2018-11-22 Buy
Alfa Aesar A13543 Guanidine hydrochloride, 98% 50-01-1 1000g $53.5 2018-11-13 Buy

Guanidine hydrochloride Chemical Properties,Uses,Production


Guanidine hydrochloride is a strong organic base existing primarily as guanidium ions at physiological pH. It is found in the urine as a normal product of protein metabolism. It is also used in laboratory research as a protein denaturant.It is also used in the treatment of myasthenia and as a fluorescent probe in HPLC.
Guanidine Hydrochloride is the hydrochloride salt form of guanidine, a strong basic compound with parasympathomimetic activity. Guanidine hydrochloride enhances the release of acetylcholine following a nerve impulse and potentiates acetylcholine actions on muscarinic and nicotinic receptors. It also appears to slow the rates of depolarization and repolarization of muscle cell membranes.
Guanidine Hydrochloride is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids. Guanidine Hydrochloride is useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins and isolation of RNA. At higher concentrations, this strong denaturant has been reported to solubilize denatured insoluble proteins such as inclusion bodies. When utilized at lower concentrations, Guanidine Hydrochloride is noted to be useful in prompting the refolding of denatured proteins. Guanidine Hydrochloride is also been reported to allow return of enzymatic activity in protocols using Guanidine Hydrochloride as a first step. Guanidine Hydrochloride is an inhibitor of RNase.
Guanidine hydrochloride is used for purification of proteins and nucleic acids. It can be used as a medicine, organic synthesis intermediate and is used in dyes. It acts as an intermediate in the preparation of sulfadiazine, which is an important raw material for sulfamethyldiazine, sulfamethazine and folic acid. It is utilized in the synthesis of 2-amino-pyrimidine, 2-amino-6-methyl-pyrimidine and 2-amino-4,6-dimethyl-pyrimidine. Also used in RNA isolation to dissociate nucleoproteins and inhibit RNase.

Protein Denaturation

Guanidine Hydrochloride (GdnHCl) is a better denaturant than urea.It should normally give higher and lower values for m and for Cm, respectively, but identical deltaG° values. Since m values in both denaturants can be predicted/estimated from the size of the protein (Myers et al., 1995) and delatG° should not change, yes it is possible-in principle-to predict Cm in one denaturant, knowing its value in the other.
but to have a general correlation co-efficient is almost impossible as every protein has unique folding. Though such co-relation may be found for a special protein fold, but what purpose it may serve apart from giving a tentative idea that GdnHCl or urea may be a better denaturant! If you are looking for even better denaturant than Gdn HCl then use GdnSCN, it is better and more effective.


Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Drug intermediates

Guanidine hydrochloride drug used primarily as an intermediate in the manufacture of sulfadiazine, an important raw material sulfamethyldiazine, sulfamethazine and folic acid.
Guanidine hydrochloride (or guanidine nitrate) and ethyl cyanoacetate reaction, cyclization of 2,4-diamino-6-hydroxy pyrimidine, folic acid for the synthesis of antianemic. Furthermore guanidine hydrochloride also used synthetic anti-static agent.


In Dicyanodiamide and ammonium (Ammonium chloride) as raw material, melt reaction at 170-230 ℃, guanidine hydrochloride obtained crude, refined products.

Reference quality standards

Name of Project Specifications
Pharmaceutical grade industrial grade
Appearance white crystal; white crystal
Content > 99% 99.5%
Ammonium <0.3% 0.5%
Water Insoluble substance <0.1% 0.1%
Moisture <0.3% 0.3%
Ash <0.05% 0.05%
Water-soluble test qualified; qualified
Melting range °C 184-188; 182-188
Absorbance (UV wavelength 260NM) <=0.04
Absorbance (UV wavelength 280NM) <=0.02
PH value (4% aqueous solution) 6.4 ± 0.2 6.4 ± 0.2


Chemical Properties

White cryst. powder




Guanidine has a good bacterial and fungicidal efficacy when used in low concentration and can also be used as disinfectant.


Used to synthesis medicine, pesticide, dye and other organic compound, is the important material to make sulfanilamide medicine and folacin, and can be used as anti-static agent of compound fibre.


ChEBI: An aminocarboxamidine, the parent compound of the guanidines.


Toxic by ingestion, evolves hydrogen chloride fumes on heating.

Purification Methods

Crystallise the hydrochloride from hot methanol by chilling to about -10o, with vigorous stirring. The fine crystals are filtered through fritted glass, washed with cold (-10o) methanol, dried at 50o under vacuum for 5hours. (The product is purer than that obtained by crystallisation at room temperature from methanol by adding large amounts of diethyl ether.) [Kolthoff et al. J Am Chem Soc 79 5102 1957, Beilstein 3 H 86, 3 II 71, 3 III 160, 3 IV 150.]

Guanidine hydrochloride Preparation Products And Raw materials

Raw materials

Preparation Products

Guanidine hydrochloride Suppliers

Global( 371)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Hefei TNJ Chemical Industry Co.,Ltd.
86-0551-65418684 18949823763
86-0551-65418684 China 1730 55
Shenzhen Sendi Biotechnology Co.Ltd.
0755-23311925 18102838259
0755-23311925 CHINA 3203 55
Henan DaKen Chemical CO.,LTD.
+86-371-55531817 CHINA 21968 58
Henan Tianfu Chemical Co.,Ltd.
0371-55170693 CHINA 20680 55
Mainchem Co., Ltd.
+86-0592-6210733 CHINA 32457 55
Xiamen AmoyChem Co., Ltd
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Chemwill Asia Co.,Ltd.
86-21-51861608;;; CHINA 24001 58
Hubei Jusheng Technology Co.,Ltd.
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Haihang Industry Co.,Ltd
+86 531 8582 1093 CHINA 4669 58
Hebei Chisure Biotechnology Co., Ltd.
+8613292893290 CHINA 1020 58

View Lastest Price from Guanidine hydrochloride manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2018-12-17 Guanidine hydrochloride
US $7.00 / kg 1kg 99% 100KG career henan chemical co
2018-12-17 Guanidine hydrochloride
US $7.00 / kg 1kg 99% 100KG career henan chemical co
2018-08-04 Guanidine hydrochloride
US $500.00 / KG 1KG 98% 10tons career henan chemical co

Guanidine hydrochloride Spectrum

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