Trometamol

Tris(hydroxymethyl)aminomethane Struktur
77-86-1
CAS-Nr.
77-86-1
Bezeichnung:
Trometamol
Englisch Name:
Tris(hydroxymethyl)aminomethane
Synonyma:
TRIS;TROMETHAMINE;TROMETAMOL;TRIS(HYDROXYMETHYL)AMINOMETHANE;2-AMINO-2-(HYDROXYMETHYL)PROPANE-1,3-DIOL;TRIS BUFFER;TRIZMA BASE;Trizma;TRIS AMINO;THAM
CBNumber:
CB8336488
Summenformel:
C4H11NO3
Molgewicht:
121.14
MOL-Datei:
77-86-1.mol

Trometamol Eigenschaften

Schmelzpunkt:
167-172 °C(lit.)
Siedepunkt:
219-220 °C10 mm Hg(lit.)
Dichte
1,353 g/cm3
Dampfdruck
0.0267 hPa (20 °C)
Brechungsindex
1.4170 (estimate)
Flammpunkt:
219-220°C/10mm
storage temp. 
20-25°C
Löslichkeit
H2O: 4 M at 20 °C, clear, colorless
Aggregatzustand
crystalline
Farbe
white
Säure-Base-Indikators(pH-Indikatoren)
7 - 9
PH
10.5-12.0(4 m in water, 25 °C)
pka
8.1(at 25℃)
Wasserlöslichkeit
550 g/L (25 ºC)
maximale Wellenlänge (λmax)
λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.08
Sensitive 
Hygroscopic
Merck 
14,9772
BRN 
741883
Stabilität:
Stable. Incompatible with bases, strong oxidizing agents. Protect from moisture.
InChIKey
LENZDBCJOHFCAS-UHFFFAOYSA-N
LogP
-2.515 (est)
CAS Datenbank
77-86-1(CAS DataBase Reference)
NIST chemische Informationen
1,3-Propanediol, 2-amino-2-(hydroxymethyl)-(77-86-1)
EPA chemische Informationen
Tris(hydroxymethyl)aminomethane (77-86-1)
Sicherheit
  • Risiko- und Sicherheitserklärung
  • Gefahreninformationscode (GHS)
Kennzeichnung gefährlicher Xi
R-Sätze: 36/37/38
S-Sätze: 26-36-37/39-24/25-23
WGK Germany  2
RTECS-Nr. TY2900000
3
TSCA  Yes
HazardClass  IRRITANT
HS Code  29221980
Giftige Stoffe Daten 77-86-1(Hazardous Substances Data)
Toxizität LD50 orally in Rabbit: > 5000 mg/kg LD50 dermal Rat > 5000 mg/kg
Bildanzeige (GHS) GHS hazard pictograms
Alarmwort Warnung
Gefahrenhinweise
Code Gefahrenhinweise Gefahrenklasse Abteilung Alarmwort Symbol P-Code
H315 Verursacht Hautreizungen. Hautreizung Kategorie 2 Warnung P264, P280, P302+P352, P321,P332+P313, P362
H319 Verursacht schwere Augenreizung. Schwere Augenreizung Kategorie 2 Warnung P264, P280, P305+P351+P338,P337+P313P
H335 Kann die Atemwege reizen. Spezifische Zielorgan-Toxizität (einmalige Exposition) Kategorie 3 (Atemwegsreizung) Warnung
Sicherheit
P261 Einatmen von Staub vermeiden.
P264 Nach Gebrauch gründlich waschen.
P264 Nach Gebrauch gründlich waschen.
P271 Nur im Freien oder in gut belüfteten Räumen verwenden.
P280 Schutzhandschuhe/Schutzkleidung/Augenschutz tragen.
P302+P352 BEI BERÜHRUNG MIT DER HAUT: Mit viel Wasser/... (Hersteller kann, falls zweckmäßig, ein Reinigungsmittel angeben oder, wenn Wasser eindeutig ungeeignet ist, ein alternatives Mittel empfehlen) waschen.
P305+P351+P338 BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.

Trometamol Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:

R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.

S-Sätze Betriebsanweisung:

S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S37/39:Bei der Arbeit geeignete Schutzhandschuhe und Schutzbrille/Gesichtsschutz tragen.

Chemische Eigenschaften

White, crystalline solid. PH (0.1M aqueous solution) 10.6, corrosive to copper, brass, aluminum. Solubility in water 80 g/100 cc (20C). Combustible.

Verwenden

Tris base has been used:
  • In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
  • As a component of sample buffer for SDS-PAGE
  • In the preparation of IEC (ion-exchange chromatography) buffer

Application

Tris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.
Tris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.
Tris(hydroxymethyl)aminomethane is used as a buffer in biochemistry and molecular biology laboratories. It is used as a primary standard to standardize acid solutions for chemical analysis. It finds application in cell membranes to increase permeability. As an alternative to sodium bicarbonate, it used in the treatment of metabolic acidosis. It acts as a precursor for the preparation of polymers, oxazolones and oxazolidines.

synthetische

Tris(hydroxymethyl)aminomethane is prepared industrially by the exhaustive condensation of nitromethane with formaldehyde under basic conditions (i.e. repeated Henry reactions) to produce the intermediate (HOCH2)3CNO2, which is subsequently hydrogenated to give the final product.

Allgemeine Beschreibung

Tris(hydroxymethyl)aminomethane is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

läuterung methode

Tris(hydroxymethyl)aminomethane can ordinarily be obtained in highly pure form suitable for use as an acidimetric standard. If only impure material is available, it should be crystallised from 20% EtOH, aqueous MeOH (m 171.1o) or isopropanol (m 172-173o). Dry it in a vacuum desiccator over P2O5 or CaCl2. Alternatively, it is dissolved in twice its weight of water at 55-60o, filtered, concentrated to half its volume and poured slowly, with stirring, into about twice its volume of EtOH. The crystals which separate on cooling to 3-4o are filtered off, washed with a little MeOH, air dried by suction, then finally ground and dried in a vacuum desiccator over P2O5. It has also been recrystallised from water, MeOH or aqueous MeOH, and vacuum dried at 80o for 2 days. [Beilstein 4 H 303, 4 III 857, 4 IV 1903.]

Trometamol Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte


Trometamol Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Global( 1068)Lieferanten
Firmenname Telefon E-Mail Land Produktkatalog Edge Rate
Suzhou Genemill Biotechnology Co.,Ltd
+86-0512-65917170; +8615862502491
aimeegao@genemill.com China 39 58
Jinan Qinmu Fine Chemical Co.,Ltd.
+8618660799346
sales@qinmuchem.com China 1009 58
Beijing Risun Science and Technology Limited
+8619801193650
xykj@risun.com China 11 58
Hefei TNJ Chemical Industry Co.,Ltd.
0551-65418684 +8618949823763
sales@tnjchem.com China 25363 58
Zouping mingyuan import & export trading co., ltd
+86-0543-2240078 +8618364991597
myzhao793@163.com China 994 58
Henan Allgreen Chemical Co.,LTD
+86-37155567971 +86-13633837469
info@allgreenchem.com China 5987 58
Wuhan BJM Pharm Inc
+86-027-5234-1919 +8613545198479
sales03@benjaminpharmchem.com China 667 58
Sinoway Industrial co., ltd.
0592-5800732; +8613806035118
xie@china-sinoway.com China 998 58
Henan Bao Enluo International TradeCo.,LTD
+86-17331933971 +86-17331933971
deasea125996@gmail.com China 2504 58
Hebei Yibangte Import and Export Co. , Ltd.
+86-0311-85030958 +8615532196582
lisa@yibangte.com China 2987 58

77-86-1(Trometamol)Verwandte Suche:


  • Tromethamine, USP Grade Tris, USP Grade
  • Tris(hydroxymethyl)aminomethane (TRIS)99.9%
  • Tris-hydroxymethylaminomethane: (THAM)
  • TRIS(HYDROXYMETHYL) AMINOMETHANE pure AR
  • TRIS(HYDROXYMETHYL)AMINOMETHANE extrapure AR
  • TRIS (HYDROXYMETHYL) AMINO METHANE USP
  • TRIS(HYDROXYMETHYL)AMINOMETHANE, 99.8+%, A.C.S. REAGENT
  • TRIZMA(R) BASE, BIOTECHNOLOGY PERFORMANCE CERTIFIED, CELL CULTURE TESTED MEETS EP & USP TESTING SPECIFICATIONS
  • TRIS(HYDROXYMETHYL)AMINOMETHANE, 99+%
  • TRIZMA BASE
  • TRIZMA BASE ELECTROPHORESIS REAGENT
  • TRIZMA(R) PRE-SET CRYSTALS, TYPE 7.4-FT, FISH GRADE, PH 7.4
  • TRIZMA BASETHYL), FOR MOLECULAR BIOLOGY*
  • TRIZMA BASE REAGENT GRADE
  • TRIS(HYDROXYMETHYL)AMINOMETHANE 99.8+%&
  • TRIZMA TYPE 8.3 FT
  • TRIZMA BASE SOLUTION
  • TRIZMA BASE, FOR LUMINESCENCE*
  • TRIZMA(R) BASE, 4X10 G*
  • TRIZMA(R) BASE, PH EUR
  • TRIZMA BASE SOLUTION, 1.5M
  • TRIZMA BASE BUFFER
  • TROMETHAMINE USP
  • TRIZMA BASE FOR BIOTECHNOLOGY,*DIAGNOSTI CS OR,
  • TRIS, certified standard titrimetric substance
  • TRIS(HYDROXYMETHYL)AMINOMETHANE, 99.9+%, ULTRAPURE GRADE
  • TRIZMA BASE SIGMAULTRA
  • TRIS ACIDIMETRIC NIST STANDARD
  • TRIS TRIS(HYDROXYMETHYL)AMINOMETHANE 99%
  • TRIS BUFFER 1.0 M SOLUTION, PH 7.5
  • TRIS TRIS(HYDROXYMETHYL)AMINOMETHANE BUFFER SUBSTANCE GR ACS 99.5+%
  • TRIS BUFFER 1.0 M SOLUTION, PH 9.0
  • TRIS TRIS(HYDROXYMETHYL)AMINOMETHANE BIOTECHNOLOGY GRADE 99.9+%
  • TRIS BUFFER 0.1 M SOLUTION, PH 7.4
  • TRIS BUFFER 2.0 M SOLUTION, PH 7.8
  • TRIS BUFFER 0.5 M SOLUTION, PH 6.8
  • TRIS TRIS(HYDROXYMETHYL)AMINOMETHANE MOLECULAR BIOLOGY REAGENT 99.9+%
  • TRIS BUFFER 1.0 M SOLUTION, PH 10.0
  • TRIS BUFFER (1 M) PH 8.0
  • TRIS BUFFER (1 M) PH 7.4
  • TRIS BUFFER USP 25 (TROMETHAMINE)
  • TRIS - SOLUTION FOR PHENOL EQUILIBRATION
  • TRIS ULTRAPURE (TRIS(HYDROXYMETHYL)AMINOMETHANE)
  • TRIS(HYDROXYMETHYL)AMINOMETHANE, ULTRAPURE >99.9%
  • TRIHYDROXYMETHYLAMINOMETHANE(TRIS)
  • 3-Propanediol, 2-amino-2-(hydroxymethyl)
  • Tris(hydroxymethyl)aminomethane, ACS, 99.8-100.1% (Assay, dried basis)
  • Tris(hydroxymethyl)aminomethane [for Electrophoresis]
  • 1,1,1-tris(hydroxymethyl)-methanamin
  • 1,1,1-tris(hydroxymethyl)-methylamin
  • 1,1,1-tris(hydroxymethyl)methylamine
  • 1,3-Propanediol,2-amino-2-(hydroxymethyl)-
  • Tris(hydroxymethyl)amino-methan
  • tris(hydroxymethyl)aminomethane(tris)
  • Tris(hydroxymethyl)methanamine
  • Tris, free base
  • tris[bufferingagent]
  • Tris-(hydroxymethyl)-aminomethane, buffer substance, reagent grade, ACS
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