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Pectinase

Description Sources Physical properties Classification Uses Application Preparation References
Pectinase
Pectinase structure
CAS No.
9032-75-1
Chemical Name:
Pectinase
Synonyms
Pectase;ultrazym;PECTINASE;PeCLinase;guojiaomei;EC 3.2.1.15;Pectinase SS;IUB: 4.2.2.10;Pectinex 3X L;PectinaseY-32
CBNumber:
CB6115887
Molecular Formula:
C18H37N(CH3)2
Formula Weight:
0
MOL File:
Mol file

Pectinase Properties

storage temp. 
2-8°C
form 
powder
color 
slightly beige
Water Solubility 
Soluble in water and practically insoluble in ethanol and ether.
EPA Substance Registry System
Polygalacturonase (9032-75-1)
SAFETY
  • Risk and Safety Statements
Safety Statements  24/25
WGK Germany  3
3-10
TSCA  Yes
HS Code  35079090

Pectinase price More Price(22)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 17389 Pectinase from Aspergillus niger powder, slightly beige, >1 U/mg 9032-75-1 10g $37.6 2018-11-13 Buy
Sigma-Aldrich P4716 Pectinase from Aspergillus niger BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5?units/mg protein (Lowry) 9032-75-1 5000 units $55.9 2021-03-22 Buy
Sigma-Aldrich P2401 Pectinase from Rhizopus sp. powder, 400-800?units/g solid 9032-75-1 500 units $57.5 2021-03-22 Buy
Sigma-Aldrich 17389 Pectinase from Aspergillus niger powder, slightly beige, >1 U/mg 9032-75-1 50g $86 2018-11-13 Buy
Sigma-Aldrich P4716 Pectinase from Aspergillus niger BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5?units/mg protein (Lowry) 9032-75-1 10000 units $102 2021-03-22 Buy

Pectinase Chemical Properties,Uses,Production

Description

Pectinase is a complex enzyme consisting of pectolyase, pectozyme, and polygalacturonase. It is able to break down pectin, a kind of polysaccharide existing in plant cell wall. Pectin is It functions optimally at temperatures between 45 and 55 °C and pH between 3 and 6.5. Owing to this property, Pectinase is often used in processes demanding the degradation of plant materials such as speeding up the extraction process of fruit juice. More importantly, through breaking down pectin, which is an important diet component of human being, pectinase can positively affect the digestive system, maintain intestinal bacteria balance and promote colon health. Pectinase is also useful during the production of wine for aiding the extraction of flavours from the mash. Pectinase is also used for retting. It is usually obtained from the fermentation of fungi such as Aspergillus Niger.

Sources

Many molds and small amounts of bacteria and yeasts can produce pectinase, mainly Aspergillus and Bacillus, others including Penicillium (Penicillium expansum, Penicillium griseoroseum, etc), Sclerotium rolfsii, Rhizoctonia solani, Mucor pusilus, Mucor mucedo, Cloctridium thermosaccharolyticum, Rhizopus stolonifer, Aureobasiduim pullulans, Neurospora rassa, Sporotrichum thermophile, etc. As Aspergillus niger in fungi belongs to GRAS (General Regarded As Safe), its metabolites are safe, so currently marketed food grade pectinase is mainly from Aspergillus niger, and the optimum pH value is generally in the acidic range. With the continuous improvement of molecular biology techniques, gene cloning technology can also be used to achieve expression of pectinase in other microbial hosts.

Physical properties

The molecular weight of pectinase is generally between 20kD and 60kD, and most of them exist as monomeric enzymes. Pectinase activity is usually in the range of pH 3.0 to 9.0, with an isoelectric point of 4.0 to 9.0. Among them, an optimum pH of a hydrolase is 4.0 to 6.5, and its action does not require the participation of Ca2+. The optimum pH of the penctin lyase is 8.0 to 10.0, and its action requires Ca2+ involvement. The pectinase produced by different strains has different properties. Common purification methods of pectinase include ammonium sulfate precipitation, acetone precipitation, ion exchange chromatography and gel filtration chromatography.

Classification

Pectinase can be divided into four types: protopectinase, polygalacturonase, penctin lyase (PL), and pectinesterase (PE). Protopectalase can promote the dissolution of pectin. According to its catalytic mechanism, protopectinase is divided into two types: Type A pectinase and Type B pectinase. The former mainly acts on the inner polygalacturonic acid region of protopectin. The latter mainly acts on the outer polysaccharide chains of the galacturonic acid chains and cell wall components. Polygalacturonase is a kind of pectinase which promotes the hydrolysis of galacturonic acid chain under water environment.

Uses

Pectinase, Aspergillus niger is used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation. It is used in the enzyme preparation. Used mostly to reduce viscosity, to improve filtration to clarify products, to avoid particle sedimentation and to prevent pectin gel formation in the manufacture of fruit juice and wine.

Application

An enzyme found in plant cells that breaks down the polysaccharide pectin. In life, this enzyme is involved in various metabolic functions of the plant, including the ripening process. In the kitchen, cooks use this enzyme to break down pectin to soften plant foods as a way to modify texture, make juicing easier, or to assist in the clarification of plant juices. Pectinase is also commonly used in wine making to get rid of cloudiness.

Preparation

Pectinase occurs throughout the natural world, but microbes are the primary tool through which it is made for commercial purposes. Producing microbial pectinase occurs mostly by submerging bacteria in a liquid that is continuously agitated, or via solid-state fermentation, which involves growing the microbes on a substrate. The microbes secrete the lipase, which is then purified, homogenized, and crystallized for sale.

References

http://www.xtend-life.com/information/ingredients/pectinase
https://en.wikipedia.org/wiki/Pectinase

Pectinase Preparation Products And Raw materials

Raw materials

Preparation Products


Pectinase Suppliers

Global( 229)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Shanghai Zheyan Biotech Co., Ltd.
18017610038
zheyansh@163.com CHINA 3623 58
Creative Enzymes
1-631-619-7922
1-631-938-8127 info@creative-enzymes.com United States 8748 58
Hebei Henghe Import and Export Trading Co. LTD
18032916000 +8618032935937
demi@hbtycoon.com CHINA 453 58
Henan DaKen Chemical CO.,LTD.
+86-371-66670886
info@dakenchem.com China 20918 58
Henan Tianfu Chemical Co.,Ltd.
0371-55170693
0371-55170693 info@tianfuchem.com CHINA 22607 55
career henan chemical co
+86-371-86658258
sales@coreychem.com CHINA 29954 58
Chongqing Chemdad Co., Ltd
+86-13650506873
sales@chemdad.com CHINA 37282 58
Shaanxi Dideu Medichem Co. Ltd
15336106720
029-88380327 1017@dideu.com CHINA 3979 58
Zhuozhou Wenxi import and Export Co., Ltd
+8613111626072 (WhatsApp)
Wechat: +8613111626072 Wickr me: waynehu zzwenxi@126.com CHINA 13187 58
Hubei Ipure Biology Co., Ltd
+8618062405514
18062427325 ada@ipurechemical.com;ada@ipurechemical.com China 10352 58

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View Lastest Price from Pectinase manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2021-11-15 Pectase; Polygalacturonase
9032-75-1
US $20.00 / KG 1000KG 99.9% 100tons Hebei Yirun Sega Biological Technology Co. Ltd
2021-10-16 Pectinase
9032-75-1
US $10.00 / KG 1KG 99% 20 tons Hebei Zhanyao Biotechnology Co. Ltd
2021-09-29 Pectinase
9032-75-1
US $0.00-0.00 / g 1g 98.0% 1kg/month WUHAN FORTUNA CHEMICAL CO., LTD

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