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Crystal Violet

Physical and Chemical Properties Application
Crystal Violet
Crystal Violet structure
CAS No.
548-62-9
Chemical Name:
Crystal Violet
Synonyms
304;vioL;badil;axuris;vianin;viocid;Crysta;atmonil;avermin;gentian
CBNumber:
CB2161846
Molecular Formula:
C25H30ClN3
Formula Weight:
407.99
MOL File:
548-62-9.mol

Crystal Violet Properties

Melting point:
205 °C (dec.) (lit.)
Boiling point:
560.86°C (rough estimate)
Density 
1.19 g/cm3 at 20 °C
refractive index 
1.6010 (estimate)
Flash point:
40 °C
storage temp. 
room temp
solubility 
water: soluble50g/L at 27°C
Colour Index 
42555
pka
9.4(at 25℃)
form 
Solid
color 
S. No.: 785
PH Range
0.8(yellow)-2.6(blue/violet)
Odor
Slight characteristic odor
PH
2.5-3.5 (10g/l, H2O, 20℃)
Water Solubility 
16 g/L (25 ºC)
λmax
590nm
Merck 
14,4395
BRN 
4077708
Stability:
Stable. Incompatible with strong oxidizing agents, strong acids. Light-sensitive. Combustible.
Biological Applications
Detecting microorganisms; treating atopic dermatitis,dermatological diseases,28,skin wounds,lesions,hemorrhoids,1,multiple myeloma,Non-Hodgkin’s lymphoma,breast cancer,neurodegenerative diseases,onychomycosis; wound dressing
Major Application
Photoresists, lithographic printing plate, printed circuit board, inks, hair dyes, shampoo, drug screening method, bone cement preparation method, microorganisms, hemorrhoids, antifungal, antibacterial, antimalarial agent, dental application
InChIKey
ZXJXZNDDNMQXFV-UHFFFAOYSA-M
CAS DataBase Reference
548-62-9(CAS DataBase Reference)
FDA 21 CFR
589.1000
EWG's Food Scores
4-7
Proposition 65 List
Gentian Violet
ATC code
D01AE02,G01AX09
EPA Substance Registry System
Crystal violet (548-62-9)
SAFETY
  • Risk and Safety Statements
Symbol(GHS) 
GHS05,GHS07,GHS08,GHS09,GHS02
Signal word  Danger
Hazard statements  H302-H318-H350-H410-H319-H351-H411-H226-H341-H400-H361-H373
Precautionary statements  P202-P260-P264-P270-P391-P405-P201-P280-P301+P312+P330-P305+P351+P338+P310-P308+P313-P273-P281-P305+P351+P338-P501-P310a-P501a
Hazard Codes  Xn,N,T,C
Risk Statements  52/53-50/53-45-41-22-40-34-10-36/37/38-23/25-51/53-68
Safety Statements  61-60-53-45-46-36/37/39-26-39-36/37-16-22
RIDADR  UN 3077 9/PG 3
WGK Germany  3
RTECS  BO9000000
TSCA  Yes
HazardClass  9
PackingGroup  III
HS Code  32041300
Toxicity LD50 orally in mice, rats: 1.2, 1.0 g/kg (Hodge)
NFPA 704
0
2 0

Crystal Violet price More Price(83)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich HT90132 Crystal violet solution 548-62-9 8 fL oz $51.5 2021-12-16 Buy
Sigma-Aldrich 1.01408 Crystal violet (C.I. 42555) indicatorACS,Reag.PhEur 548-62-9 25 g $62.25 2021-12-16 Buy
Sigma-Aldrich HT901 Crystal violet solution 548-62-9 1 L $91.4 2021-12-16 Buy
Sigma-Aldrich 1.15940 Crystal violet (C.I. 42555) formicroscopyCertistain? 548-62-9 25 g $119.32 2021-12-16 Buy
Sigma-Aldrich 1.01408 Crystal violet (C.I. 42555) indicatorACS,Reag.PhEur 548-62-9 100 g $220.15 2021-12-16 Buy

Crystal Violet Chemical Properties,Uses,Production

Physical and Chemical Properties

It is also known as "purple", "methyl violet", "gentian violet." It belongs to triphenylmethane type alkaline dyes. Scientific name:"hexamethyl chloride rose aniline." It appears as dark green powder with metallic luster. It is soluble in water, alcohol and chloroform, but insoluble in ether. Its solubility in water is 1.68% and its solubility in 95% ethanol is 13.87%. However, the solubility of its iodide in two solvents is only 0.035% and 1.78% respectively. Both its aqueous solution and alcohol solution are purple. The structural formula of its chloride is:

Application

It can be used as the dyes of silk, paper and acrylic as well as biological stains. It can be used for manufacturing paints and printing inks. It can also used as an acid-alkaline indicator with coloring range being from pH 0.5 (green) to 2.0 (blue), and developing reagent for colorimetric assays.
It can form a colored chelate with thallium in the hydrobromic acid medium, thus it can be used as thallium sensitivity reagent. It can also be used for the determination of other metal ions such as zinc, antimony, titanium, cadmium, tungsten, gold, mercury and so on. It can also be used as biological stains and non-aqueous titration acid-base indicator. In addition, it can also be used as antiseptic for inhibiting Staphylococcus aureus, Streptococcus and some fungi. Its 1% aqueous solution is commonly known as "purple syrup" for the prevention and treatment of skin and mucous membrane infections. Its enteric-coated tablets can be used as orally anti-pinworm medicine.

Description

Crystal Violet is light sensitive. May react vigorously with strong oxidizing agents. May react exothermically with reducing agents to release gaseous hydrogen.

Description

Anti-infective (topical). Has been used as anthelmintic (Nematodes), as indicator for copper salts.

Chemical Properties

dark green powder or crystals

Chemical Properties

This is a perennial, herbaceous plant native to mountainous areas of Europe; it may reach 0.5 to 1.0 m (2 to 3 ft) in height It has large, cylindrical roots (internally yellow); erect chalice; and fowers with a yellow corolla and peduncles It blooms from July to August The part used is the root of two-year-old plants The color of the rhizomes ranges from dark brown to light tan The color of roots appears to be related to its bitter principle content The dried product and its derivatives of gentian exhibit a very bitter favor.

Occurrence

Gentian is a fl owering perennial found in Europe and Asia.

Uses

Crystal Violet is used as an acid-base indicator, an alcohol denaturant, a biological stain, a textile dye and an indicator for copper salts. It is a component of gram staining in microorganisms and cell lines.

Uses

As dye for wood, silk, paper; in inks; as biological stain.

Preparation

It is manufactured through using N, N-dimethylaniline as raw materials, followed by condensation, addition, chlorination and other reactions. Alternatively, it can be synthesized through the reaction between Michler ketone and N, N-dimethylaniline reaction in the presence of phosphorus oxychloride, followed by azeotropic reaction with hydrochloric acid. Recrystallization in hot water will generate compound containing nine crystal water molecules.

brand name

Genapax (Key); Gvs (Savage).

Essential oil composition

A number of bitter compounds present in gentian are primarily amarogentin (strongly bitter), gentiopricin (approximately 1.5% in fresh root), swertiamarin and gentiopricroside. The leaves and flowers contain mainly xanthones. Secoiridoids and flavonoids were also detected. In the phase of flowering, leaves are rich with compounds possessing C-glycoside structures while O-glycoside structures accumulate mainly before flowering.

General Description

It belongs to triamino-triphenylmethane synthetic dyes; alkaline. It is an important dye used in bacterial Gram stain. The mixed reagent of crystal violet chloride and iodine is known as gentian violet.

Air & Water Reactions

Insoluble in water.

Fire Hazard

Flash point data for Crystal Violet are not available, however, Crystal Violet is probably combustible.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

Clinical Use

Gentian violet is variously known as hexamethyl-p-rosanilinechloride, crystal violet, methyl violet, and methylrosanilinechloride. It occurs as a green powder or greenflakes with a metallic sheen. The compound is soluble inwater (1:35) and alcohol (1:10) but insoluble in nonpolar organicsolvents. Gentian violet is available in vaginal suppositoriesfor the treatment of yeast infections. It is also used asa 1% to 3% solution for the treatment of ringworm and yeastinfections. Gentian violet has also been used orally as an anthelminticfor strongyloidiasis (threadworm) and oxyuriasis.

Safety Profile

Poison by ingestion, intravenous, and intraperitoneal routes, An experimental teratogen. Other experimental reproductive effects. A human sktn irritant. Human mutation data reported. Questionable carcinogen with experimental carcinogenic data. When heated to decomposition it emits very toxic fumes of NO, and Cl-.

Properties and Applications

TEST ITEMS

SPECIFICATION

APPEARANCE

DARK GREEN POWDER OR GRANULE

SHADE

BLUISH

HEAT RESISTANCE

200 °C min

DENSITY

1.07 g/cm 3

WATER FASTNESS

4-5

LIGHT FASTNESS

1-2

BLEACHABILITY (OXIDATIVE)

4

BLEACHABILITY (REDUCTIVE)

3

WATER SOLUBILITY AT 25 °C

16 g/L min

WATER INSOLUBLE

1.0% max

MOISTURE

3.0% max

TINTING STRENGTH

100-105 %

WEIGHT METAL TOTAL

50ppm max

Purification Methods

Crystallise the dye from water (20mL/g), the crystals being separated from the chilled solution by centrifugation, then wash them with chilled EtOH (solubility is 1g in 10 mL of hot EtOH) and diethyl ether and dry under vacuum. It is soluble in CHCl3 but insoluble in Et2O. The carbinol is precipitated from an aqueous solution of the dye-hydrochloride, using excess NaOH, then dissolve in HCl and recrystallise it from water as the chloride [UV and kinetics: Turgeon & La Mer J Am Chem Soc 74 5988 1952]. The carbinol base has m 195o (needles from EtOH). The diphthalate (blue and turns red in H2O) crystallises from H2O, m 153-154o(dec at 185-187o)[Chamberlain & Dull J Am Chem Soc 50 3089 1928]. [Beilstein 13 H 233, 13 IV 2284.]

Crystal Violet Preparation Products And Raw materials

Raw materials

Preparation Products


Crystal Violet Suppliers

Global( 461)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Henan Tianfu Chemical Co.,Ltd.
0371-55170693
0371-55170693 info@tianfuchem.com China 22607 55
Shanghai Time Chemicals CO., Ltd.
+8618017249410 +86-021-57951555
+86-021-57951555 jack.li@time-chemicals.com China 1807 55
ATK CHEMICAL COMPANY LIMITED
+86 21 5161 9050/ 5187 7795
+86 21 5161 9052/ 5187 7796 ivan@atkchemical.com CHINA 26782 60
career henan chemical co
+86-0371-55982848
sales@coreychem.com China 29954 58
Hubei Jusheng Technology Co.,Ltd.
86-18871470254
027-59599243 linda@hubeijusheng.com CHINA 28229 58
Hubei xin bonus chemical co. LTD
86-13657291602
027-59338440 linda@hubeijusheng.com CHINA 23035 58
Shandong chuangyingchemical Co., Ltd.
18853181302
sale@chuangyingchem.com CHINA 5917 58
Chongqing Chemdad Co., Ltd
+86-13650506873
sales@chemdad.com CHINA 37282 58
Winchem industrial co limited
0086-574-83851061
0086-574-87083209 info@win-chemical.com CHINA 300 58
CONIER CHEM AND PHARMA LIMITED
86-18523575427
sales@conier.com China 47498 58

View Lastest Price from Crystal Violet manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2021-09-29 Crystal Violet
548-62-9
US $1.00 / KG 1KG 96.0%-100.5%,USP40 5tons/month WUHAN FORTUNA CHEMICAL CO., LTD
2021-07-13 Crystal Violet
548-62-9
US $15.00-10.00 / KG 1KG 99%+ HPLC Monthly supply of 1 ton Zhuozhou Wenxi import and Export Co., Ltd
2021-07-10 Crystal Violet
548-62-9
US $15.00-10.00 / KG 1KG 99%+ HPLC Monthly supply of 1 ton Zhuozhou Wenxi import and Export Co., Ltd

Crystal Violet Spectrum


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